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Registro Completo |
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
29/01/2018 |
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Data da última atualização: |
06/08/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FREITAS, S. T. de; AMARANTE, C. V. T. do; DANDEKAR, A. M.; MITCHAM, E. J. |
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Afiliação: |
SERGIO TONETTO DE FREITAS, CPATSA; CASSANDRO VIDAL TALAMINI DO AMARANTE, UFSC; A.M. Dandekar, Department of Plant Sciences, University of California, Davis, CA 95616, USA.; E.J. Mitcham, Department of Plant Sciences, University of California, Davis, CA 95616, USA. |
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Título: |
Mechanisms regulating bitter pit development in Greensleeves apples with suppression of ethylene biosynthesis. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Acta Horticulturae, Leuven, n. 1179, p. 61-68, nov. 2017. |
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ISSN: |
978-94-62611-75-7 |
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DOI: |
10.17660/ActaHortic.2017.1179.10 |
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Idioma: |
Português |
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Conteúdo: |
Bitter pit (BP) is a physiological disorder in apple believed to be triggered by low fruit Ca2+ content and enhanced by ethylene. The objectives of this study were to understand the role of ethylene, Ca2+ binding to the cell wall and the ratio between Ca2+ and other nutrients (Mg2+, K+ and N) on BP development in wild-type (GS) and ethylene-suppressed (68G and 103Y) ?Greensleeves? apples. The transgenic line 68G is suppressed for 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase (ACO), and line 103Y is suppressed for ACC synthase (ACS). Suppression of ethylene biosynthesis reduced the incidence and severity of BP, and decreased the rate of fruit softening and the degree of cell-wall pectin de-esterification in fruit cortical tissue. Lower ethylene biosynthesis, in ethylene-suppressed genotypes, had no effect on Ca2+, Mg2+, K+ or N concentrations in cortical tissue, on Ca2+ concentrations in the cell wall, on expression of pectin methylesterases (PME1 and PME2) or on percentages of cortical Ca2+ bound to the cell wall. In all genotypes, fruit with BP had lower Ca2+ and higher Mg2+ concentrations and higher Mg2+/Ca2+ ratios in cortical tissue, as well as higher percentages of cortical Ca2+ bound to the cell wall, compared with fruit without BP. Fruit with and without BP had similar cell-wall Ca2+ concentrations, and cell-wall content and levels of PME expression in cortical tissue. The results indicate that high levels of ethylene biosynthesis, Ca2+ binding to the cell wall and Mg2+ in cortical tissue can enhance fruit susceptibility to BP. MenosBitter pit (BP) is a physiological disorder in apple believed to be triggered by low fruit Ca2+ content and enhanced by ethylene. The objectives of this study were to understand the role of ethylene, Ca2+ binding to the cell wall and the ratio between Ca2+ and other nutrients (Mg2+, K+ and N) on BP development in wild-type (GS) and ethylene-suppressed (68G and 103Y) ?Greensleeves? apples. The transgenic line 68G is suppressed for 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase (ACO), and line 103Y is suppressed for ACC synthase (ACS). Suppression of ethylene biosynthesis reduced the incidence and severity of BP, and decreased the rate of fruit softening and the degree of cell-wall pectin de-esterification in fruit cortical tissue. Lower ethylene biosynthesis, in ethylene-suppressed genotypes, had no effect on Ca2+, Mg2+, K+ or N concentrations in cortical tissue, on Ca2+ concentrations in the cell wall, on expression of pectin methylesterases (PME1 and PME2) or on percentages of cortical Ca2+ bound to the cell wall. In all genotypes, fruit with BP had lower Ca2+ and higher Mg2+ concentrations and higher Mg2+/Ca2+ ratios in cortical tissue, as well as higher percentages of cortical Ca2+ bound to the cell wall, compared with fruit without BP. Fruit with and without BP had similar cell-wall Ca2+ concentrations, and cell-wall content and levels of PME expression in cortical tissue. The results indicate that high levels of ethylene biosynthesis, Ca2+ binding to the cell wall ... Mostrar Tudo |
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Palavras-Chave: |
Deficiencia de cálcio; Desordem fisiológica. |
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Thesagro: |
Cálcio; Fisiologia Nutricional; Maça; Malus Domestica; Pós-Colheita. |
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Thesaurus Nal: |
Ethylene; Genetic disorders; Physiological response; Postharvest treatment. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02533naa a2200313 a 4500
001 2086550
005 2019-08-06
008 2017 bl uuuu u00u1 u #d
022 $a978-94-62611-75-7
024 7 $a10.17660/ActaHortic.2017.1179.10$2DOI
100 1 $aFREITAS, S. T. de
245 $aMechanisms regulating bitter pit development in Greensleeves apples with suppression of ethylene biosynthesis.$h[electronic resource]
260 $c2017
520 $aBitter pit (BP) is a physiological disorder in apple believed to be triggered by low fruit Ca2+ content and enhanced by ethylene. The objectives of this study were to understand the role of ethylene, Ca2+ binding to the cell wall and the ratio between Ca2+ and other nutrients (Mg2+, K+ and N) on BP development in wild-type (GS) and ethylene-suppressed (68G and 103Y) ?Greensleeves? apples. The transgenic line 68G is suppressed for 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase (ACO), and line 103Y is suppressed for ACC synthase (ACS). Suppression of ethylene biosynthesis reduced the incidence and severity of BP, and decreased the rate of fruit softening and the degree of cell-wall pectin de-esterification in fruit cortical tissue. Lower ethylene biosynthesis, in ethylene-suppressed genotypes, had no effect on Ca2+, Mg2+, K+ or N concentrations in cortical tissue, on Ca2+ concentrations in the cell wall, on expression of pectin methylesterases (PME1 and PME2) or on percentages of cortical Ca2+ bound to the cell wall. In all genotypes, fruit with BP had lower Ca2+ and higher Mg2+ concentrations and higher Mg2+/Ca2+ ratios in cortical tissue, as well as higher percentages of cortical Ca2+ bound to the cell wall, compared with fruit without BP. Fruit with and without BP had similar cell-wall Ca2+ concentrations, and cell-wall content and levels of PME expression in cortical tissue. The results indicate that high levels of ethylene biosynthesis, Ca2+ binding to the cell wall and Mg2+ in cortical tissue can enhance fruit susceptibility to BP.
650 $aEthylene
650 $aGenetic disorders
650 $aPhysiological response
650 $aPostharvest treatment
650 $aCálcio
650 $aFisiologia Nutricional
650 $aMaça
650 $aMalus Domestica
650 $aPós-Colheita
653 $aDeficiencia de cálcio
653 $aDesordem fisiológica
700 1 $aAMARANTE, C. V. T. do
700 1 $aDANDEKAR, A. M.
700 1 $aMITCHAM, E. J.
773 $tActa Horticulturae, Leuven$gn. 1179, p. 61-68, nov. 2017.
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Embrapa Semiárido (CPATSA) |
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