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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
26/11/1999 |
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Data da última atualização: |
15/05/2023 |
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Autoria: |
MIRANDA, M.; MOTOMURA, T.; IKEDA, F.; OHGAWARA, T.; SAITO, W.; ENDO, T.; OMURA, M.; MORIGUCHI, T. |
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Título: |
Somatic hybrids obtained by fusion between Poncirus trifoliata (2x) and fortunella hindsii (4x) protoplasts. |
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Ano de publicação: |
1997 |
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Fonte/Imprenta: |
Plant Cell Reports, v.16, p.401-405, 1997. |
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Idioma: |
Inglês |
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Conteúdo: |
Somatic hybrids were obtained by the symmetric fusion of embryogenic callus cells from tetraploid `Mame' kumquat [Fortunella hindsii (Champ.) Swing.] and mesophyll cells from diploid trifoliate orange [Poncirus trifoliata (L.) Raf.]. Southern blot analysis of three regenerants revealed that they carried specific rDNA fragments from both fusion partners, thereby confirming their hybridity. In contrast, mitochondrial DNA (mtDNA) and chloroblast DNA (cpDNA) were unidirectionally transmitted from the callus parent without any evidence of recombination. No differences in the restriction fragment patterns of rDNA, mtDNA or cpDNA could be detected among the regenerants. Flow cytometry showed that two regenerants were hexaploids, as expected, but that one was pentaploid, probably due to elimination of chromosomes prior to the regeneration of this plant. |
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Palavras-Chave: |
Citros; Eletrofusao; Eliminacao de cromossomos; Poliplaides. |
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Thesaurus Nal: |
chromosome elimination; Citrus; electrofusion; flow cytometry; polyploidy. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01714naa a2200313 a 4500
001 1648429
005 2023-05-15
008 1997 bl uuuu u00u1 u #d
100 1 $aMIRANDA, M.
245 $aSomatic hybrids obtained by fusion between Poncirus trifoliata (2x) and fortunella hindsii (4x) protoplasts.$h[electronic resource]
260 $c1997
520 $aSomatic hybrids were obtained by the symmetric fusion of embryogenic callus cells from tetraploid `Mame' kumquat [Fortunella hindsii (Champ.) Swing.] and mesophyll cells from diploid trifoliate orange [Poncirus trifoliata (L.) Raf.]. Southern blot analysis of three regenerants revealed that they carried specific rDNA fragments from both fusion partners, thereby confirming their hybridity. In contrast, mitochondrial DNA (mtDNA) and chloroblast DNA (cpDNA) were unidirectionally transmitted from the callus parent without any evidence of recombination. No differences in the restriction fragment patterns of rDNA, mtDNA or cpDNA could be detected among the regenerants. Flow cytometry showed that two regenerants were hexaploids, as expected, but that one was pentaploid, probably due to elimination of chromosomes prior to the regeneration of this plant.
650 $achromosome elimination
650 $aCitrus
650 $aelectrofusion
650 $aflow cytometry
650 $apolyploidy
653 $aCitros
653 $aEletrofusao
653 $aEliminacao de cromossomos
653 $aPoliplaides
700 1 $aMOTOMURA, T.
700 1 $aIKEDA, F.
700 1 $aOHGAWARA, T.
700 1 $aSAITO, W.
700 1 $aENDO, T.
700 1 $aOMURA, M.
700 1 $aMORIGUCHI, T.
773 $tPlant Cell Reports$gv.16, p.401-405, 1997.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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