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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Florestas. |
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Data corrente: |
03/06/2024 |
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Data da última atualização: |
12/09/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
NASCIMENTO, S. C. do; SCHEUERMANN, K. K.; PEREIRA, F. S.; GORAYEB, E. S.; ALBUQUERQUE, M. R. M.; MENDES, G. C.; MELLO, R. N. de; LAU, D.; SILVA, F. N. da. |
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Afiliação: |
SAMARA CAMPOS DO NASCIMENTO, UNIVERSIDADE DO ESTADO DE SANTA CATARINA; KLAUS KONRAD SCHEUERMANN, EMPRESA DE PESQUISA AGROPECUÁRIA E EXTENSÃO RURAL DE SANTA CATARINA; FERNANDO SARTORI PEREIRA, UNIVERSIDADE DO ESTADO DE SANTA CATARINA; EDUARDO SILVA GORAYEB, UNIVERSIDADE DO ESTADO DE SANTA CATARINA; MATHEUS RODRIGUES MAGALHÃES ALBUQUERQUE, UNIVERSIDADE DO ESTADO DE SANTA CATARINA; GISELLE CAMARGO MENDES, INSTITUTO FEDERAL DE SANTA CATARINA; RAQUEL NEVES DE MELLO, CNPAF; DOUGLAS LAU, CNPF; FABIO NASCIMENTO DA SILVA, UNIVERSIDADE DO ESTADO DE SANTA CATARINA. |
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Título: |
Development of a severity scale and an RT‐qPCR assay for screening resistance levels in rice genotypes against rice stripe necrosis virus and its vector. |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
Plant Pathology, v. 73, n. 8, p. 2101-2111, Oct. 2024. |
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ISSN: |
1365-3059 |
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DOI: |
http://doi.org/10.1111/ppa.13952 |
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Idioma: |
Inglês |
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Conteúdo: |
Rice stripe necrosis virus (RSNV) is the causal agent of the disease ‘rice crinkling’ and is transmitted by the protozoan Polymyxa graminis. Although genetic resistance has been explored, no resistant commercial cultivars are currently available. Oryza gla- berrima has been identified as a promising source of resistance. However, it remains unclear whether this resistance is effective against the virus, the vector, or both, as well as whether it can be transferred to Oryza sativa cultivars. Disease-resistant geno- types are primarily selected through visual observations of symptom expression. The absence of a severity scale for RSNV makes this process difficult, and relying solely on visual assessments can introduce subjectivity. We developed a severity scale and a reverse transcripiton-quantitative PCR (RT-qPCR) assay for screening resistance lev- els in rice genotypes against RSNV and its vector and to analyse the genetic variability of RSNV isolates. To achieve absolute quantification, experiments were conducted using O. glaberrima and three O. sativa cultivars. Inoculation occurred naturally using soil from an area with a history of the disease. Visual symptoms were recorded and disease intensity was evaluated. Subsequently, total nucleic acid extraction was per- formed on the samples and viral and vector loads were quantified through RT-qPCR and qPCR, respectively. To characterize the virus variability, symptomatic rice sam- ples were collected in the 2021/2022 crop season. RT-PCR was conducted to amplify the coat protein gene of RSNV, and molecular variability descriptors were analysed. MenosRice stripe necrosis virus (RSNV) is the causal agent of the disease ‘rice crinkling’ and is transmitted by the protozoan Polymyxa graminis. Although genetic resistance has been explored, no resistant commercial cultivars are currently available. Oryza gla- berrima has been identified as a promising source of resistance. However, it remains unclear whether this resistance is effective against the virus, the vector, or both, as well as whether it can be transferred to Oryza sativa cultivars. Disease-resistant geno- types are primarily selected through visual observations of symptom expression. The absence of a severity scale for RSNV makes this process difficult, and relying solely on visual assessments can introduce subjectivity. We developed a severity scale and a reverse transcripiton-quantitative PCR (RT-qPCR) assay for screening resistance lev- els in rice genotypes against RSNV and its vector and to analyse the genetic variability of RSNV isolates. To achieve absolute quantification, experiments were conducted using O. glaberrima and three O. sativa cultivars. Inoculation occurred naturally using soil from an area with a history of the disease. Visual symptoms were recorded and disease intensity was evaluated. Subsequently, total nucleic acid extraction was per- formed on the samples and viral and vector loads were quantified through RT-qPCR and qPCR, respectively. To characterize the virus variability, symptomatic rice sam- ples were collected in the 2021/2022 crop sea... Mostrar Tudo |
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Palavras-Chave: |
Genetic variability. |
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Thesagro: |
Arroz; Doença de Planta; Oryza Sativa; Resistência Genética; Variação Genética. |
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Thesaurus Nal: |
Genetic resistance; Viral load. |
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Categoria do assunto: |
H Saúde e Patologia
O Insetos e Entomologia |
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Marc: |
LEADER 02678naa a2200337 a 4500
001 2165173
005 2024-09-12
008 2024 bl uuuu u00u1 u #d
022 $a1365-3059
024 7 $ahttp://doi.org/10.1111/ppa.13952$2DOI
100 1 $aNASCIMENTO, S. C. do
245 $aDevelopment of a severity scale and an RT‐qPCR assay for screening resistance levels in rice genotypes against rice stripe necrosis virus and its vector.$h[electronic resource]
260 $c2024
520 $aRice stripe necrosis virus (RSNV) is the causal agent of the disease ‘rice crinkling’ and is transmitted by the protozoan Polymyxa graminis. Although genetic resistance has been explored, no resistant commercial cultivars are currently available. Oryza gla- berrima has been identified as a promising source of resistance. However, it remains unclear whether this resistance is effective against the virus, the vector, or both, as well as whether it can be transferred to Oryza sativa cultivars. Disease-resistant geno- types are primarily selected through visual observations of symptom expression. The absence of a severity scale for RSNV makes this process difficult, and relying solely on visual assessments can introduce subjectivity. We developed a severity scale and a reverse transcripiton-quantitative PCR (RT-qPCR) assay for screening resistance lev- els in rice genotypes against RSNV and its vector and to analyse the genetic variability of RSNV isolates. To achieve absolute quantification, experiments were conducted using O. glaberrima and three O. sativa cultivars. Inoculation occurred naturally using soil from an area with a history of the disease. Visual symptoms were recorded and disease intensity was evaluated. Subsequently, total nucleic acid extraction was per- formed on the samples and viral and vector loads were quantified through RT-qPCR and qPCR, respectively. To characterize the virus variability, symptomatic rice sam- ples were collected in the 2021/2022 crop season. RT-PCR was conducted to amplify the coat protein gene of RSNV, and molecular variability descriptors were analysed.
650 $aGenetic resistance
650 $aViral load
650 $aArroz
650 $aDoença de Planta
650 $aOryza Sativa
650 $aResistência Genética
650 $aVariação Genética
653 $aGenetic variability
700 1 $aSCHEUERMANN, K. K.
700 1 $aPEREIRA, F. S.
700 1 $aGORAYEB, E. S.
700 1 $aALBUQUERQUE, M. R. M.
700 1 $aMENDES, G. C.
700 1 $aMELLO, R. N. de
700 1 $aLAU, D.
700 1 $aSILVA, F. N. da
773 $tPlant Pathology$gv. 73, n. 8, p. 2101-2111, Oct. 2024.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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