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 | Acesso ao texto completo restrito à biblioteca da Embrapa Semiárido. Para informações adicionais entre em contato com cpatsa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Instrumentação; Embrapa Semiárido. |
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Data corrente: |
28/09/2018 |
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Data da última atualização: |
03/10/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FERREIRA, L. M. B.; ALONSO, J. D.; KIILL, C. P.; FERREIRA, N. N.; BUZZÁ, H. H.; GODOI, D. R. M. de; BRITTO, D. de; ASSIS, O. B. G. de; SERAPHIM, T. V.; BORGES, J. C.; GREMIÃO, M. P. D. |
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Afiliação: |
DOUGLAS DE BRITTO, CPATSA; ODILIO BENEDITO GARRIDO DE ASSIS, CNPDIA. |
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Título: |
Exploiting supramolecular interactions to produce bevacizumab-loaded nanoparticles for potential mucosal delivery. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
European Polymer Journal, v. 103, p. 238-250, jun. 2018. |
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Páginas: |
238-250 |
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DOI: |
10.1016/j.eurpolymj.2018.04.013 |
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Idioma: |
Inglês |
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Conteúdo: |
Monoclonal antibody (mAb) delivery is gaining importance for local, systemic, and route-specific targeting. Themucus constitutes the main barrier for this type of delivery. In the present study, we aimed to develop a drugdelivery platform by integrating mucus penetrating and mucoadhesive agents into a single system. Our hy-pothesis is that by combining these opposing functions, this system could have its properties modulated ac-cording to specific purposes. Self-assembly studies were conducted ung three classes of building blocks: the protein drug bevacizumab (BVZ), mucus-penetrating polyanion dextran sulfate (DS), mucoadhesive polycations trimethylchitosan (TMC) and chitosan oligosaccharides (COS). We obtained two types of nanoarticles by manipulating supramolecular interactions between the components. Binary protein-polyanion (BVZ/DS) nano-particles showed size of approximately 150 nm and a negative zeta potential. Ternary protein-polyanion-poly-cation (BVZ/DS/COS) nanoparticles were obtained using COS and exhibited 350 nm and a positive zeta potential. Assisted by calorimetric information, we demonstrated that building stable ternary nanoparticles carrying positive charges were not possible using the polycation TMC due to its thermodynamic onstraints. Furthermore, spectroscopy analysis and CAM assay indicated that BVZ continued structurally and functionally stable after its incorporation into the nanoparticles. These two types of nanoparticles exhibited different be-haviors when interacting with mucin, as shown by DLS and AFM studies. While the negatively charged particles promoted dispersion of the mucin network, suggesting a mucus penetrating effect of DS, the positively chargedparticles formed aggregates, probably caused by the mucoadhesive effect of COS. These results highlight the importance of understanding the role of supramolecular interactions, responsible for forming drug delivery systems containing complex molecules, such as proteins and polymers. MenosMonoclonal antibody (mAb) delivery is gaining importance for local, systemic, and route-specific targeting. Themucus constitutes the main barrier for this type of delivery. In the present study, we aimed to develop a drugdelivery platform by integrating mucus penetrating and mucoadhesive agents into a single system. Our hy-pothesis is that by combining these opposing functions, this system could have its properties modulated ac-cording to specific purposes. Self-assembly studies were conducted ung three classes of building blocks: the protein drug bevacizumab (BVZ), mucus-penetrating polyanion dextran sulfate (DS), mucoadhesive polycations trimethylchitosan (TMC) and chitosan oligosaccharides (COS). We obtained two types of nanoarticles by manipulating supramolecular interactions between the components. Binary protein-polyanion (BVZ/DS) nano-particles showed size of approximately 150 nm and a negative zeta potential. Ternary protein-polyanion-poly-cation (BVZ/DS/COS) nanoparticles were obtained using COS and exhibited 350 nm and a positive zeta potential. Assisted by calorimetric information, we demonstrated that building stable ternary nanoparticles carrying positive charges were not possible using the polycation TMC due to its thermodynamic onstraints. Furthermore, spectroscopy analysis and CAM assay indicated that BVZ continued structurally and functionally stable after its incorporation into the nanoparticles. These two types of nanoparticles exhibited di@... Mostrar Tudo |
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Palavras-Chave: |
Bevacizumab; Drug delivery platform; Interações supramoleculares; Mucoadesão; Mucoadhesion; Nanoparticula; Nanopartículas poliméricas; Penetração do muco; Plataforma de entrega de medicamentos; Polymeric nanoparticles; Supramolecular interactions. |
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Categoria do assunto: |
--
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 03262naa a2200397 a 4500
001 2096760
005 2018-10-03
008 2018 bl uuuu u00u1 u #d
024 7 $a10.1016/j.eurpolymj.2018.04.013$2DOI
100 1 $aFERREIRA, L. M. B.
245 $aExploiting supramolecular interactions to produce bevacizumab-loaded nanoparticles for potential mucosal delivery.$h[electronic resource]
260 $c2018
300 $a238-250
520 $aMonoclonal antibody (mAb) delivery is gaining importance for local, systemic, and route-specific targeting. Themucus constitutes the main barrier for this type of delivery. In the present study, we aimed to develop a drugdelivery platform by integrating mucus penetrating and mucoadhesive agents into a single system. Our hy-pothesis is that by combining these opposing functions, this system could have its properties modulated ac-cording to specific purposes. Self-assembly studies were conducted ung three classes of building blocks: the protein drug bevacizumab (BVZ), mucus-penetrating polyanion dextran sulfate (DS), mucoadhesive polycations trimethylchitosan (TMC) and chitosan oligosaccharides (COS). We obtained two types of nanoarticles by manipulating supramolecular interactions between the components. Binary protein-polyanion (BVZ/DS) nano-particles showed size of approximately 150 nm and a negative zeta potential. Ternary protein-polyanion-poly-cation (BVZ/DS/COS) nanoparticles were obtained using COS and exhibited 350 nm and a positive zeta potential. Assisted by calorimetric information, we demonstrated that building stable ternary nanoparticles carrying positive charges were not possible using the polycation TMC due to its thermodynamic onstraints. Furthermore, spectroscopy analysis and CAM assay indicated that BVZ continued structurally and functionally stable after its incorporation into the nanoparticles. These two types of nanoparticles exhibited different be-haviors when interacting with mucin, as shown by DLS and AFM studies. While the negatively charged particles promoted dispersion of the mucin network, suggesting a mucus penetrating effect of DS, the positively chargedparticles formed aggregates, probably caused by the mucoadhesive effect of COS. These results highlight the importance of understanding the role of supramolecular interactions, responsible for forming drug delivery systems containing complex molecules, such as proteins and polymers.
653 $aBevacizumab
653 $aDrug delivery platform
653 $aInterações supramoleculares
653 $aMucoadesão
653 $aMucoadhesion
653 $aNanoparticula
653 $aNanopartículas poliméricas
653 $aPenetração do muco
653 $aPlataforma de entrega de medicamentos
653 $aPolymeric nanoparticles
653 $aSupramolecular interactions
700 1 $aALONSO, J. D.
700 1 $aKIILL, C. P.
700 1 $aFERREIRA, N. N.
700 1 $aBUZZÁ, H. H.
700 1 $aGODOI, D. R. M. de
700 1 $aBRITTO, D. de
700 1 $aASSIS, O. B. G. de
700 1 $aSERAPHIM, T. V.
700 1 $aBORGES, J. C.
700 1 $aGREMIÃO, M. P. D.
773 $tEuropean Polymer Journal$gv. 103, p. 238-250, jun. 2018.
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Embrapa Semiárido (CPATSA) |
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| 1. |  | SOARES, J. M.; VIEIRA, V. J. de S.; GOMES JÚNIOR, W. F.; ARAÚJO FILHO, A. A. de. Agrovale, uma experiência de 25 anos em irrigação da cana-de-açúcar na região do Submédio São Francisco. Item, Brasília, DF, n. 60, p. 55-62, 2003.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - C |
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