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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
24/01/2017 |
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Data da última atualização: |
04/12/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
KAYA, E.; SOUZA, F. V. D.; GÖKDOGAN, E. Y.; CEYLAN, M.; JENDEREK, M. M. |
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Afiliação: |
ERGUN KAYA, Faculty of Science, Mu?la S?tk? Koçman University; FERNANDA VIDIGAL DUARTE SOUZA, CNPMF; EMEL YILMAZ GÖKDO?AN, Faculty of Science, Mu?la S?tk? Koçman University; MUAMMER CEYLAN, Faculty of Science, Mu?la S?tk? Koçman University; MARIA M. JENDEREK, USDA-ARS. |
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Título: |
Cryopreservation of citrus seed via dehydration followed by immersion in liquid nitrogen. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Turkish Journal of Biology, 2017 |
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ISSN: |
1300-0152 |
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Idioma: |
Inglês |
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Conteúdo: |
An important method for plant germplasm conservation is offered by a biotechnology-based approach of cryopreservation. Cryopreservation refers to the storage of plant material at ultralow temperatures in liquid nitrogen. A procedure for cryopreservation of polyembryonic seeds was improved for selected citrus cultivars from Turkey. Seed dehydration was performed at different exposure times, in sterile conditions of a laminar flow-hood. The tested cultivars showed the highest tolerance to low temperature storage when the seeds were first dehydrated to a moisture content of 21.8% for Poncirus trifoliata Raf. × Citrus sinensis Osb. and to 17.6% for Citrus limonia Osbeck. The postcryopreservation germinability ranged from 73.3% (Poncirus trifoliata Raf. × C. sinensis Osb. and Fortunella margarita (Lour.) Swingle) to 93.3% (C. jambhiri Lush.). Dehydration was beneficial for germination of seeds from all of the tested citrus species after the liquid nitrogen exposure. Seedlings derived from cryopreserved seeds had well-formed shoots and roots and were easily acclimated to greenhouse conditions. |
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Thesagro: |
Criopreservação. |
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Thesaurus Nal: |
Cryopreservation. |
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Categoria do assunto: |
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Marc: |
LEADER 01686naa a2200205 a 4500
001 2061663
005 2017-12-04
008 2017 bl uuuu u00u1 u #d
022 $a1300-0152
100 1 $aKAYA, E.
245 $aCryopreservation of citrus seed via dehydration followed by immersion in liquid nitrogen.$h[electronic resource]
260 $c2017
520 $aAn important method for plant germplasm conservation is offered by a biotechnology-based approach of cryopreservation. Cryopreservation refers to the storage of plant material at ultralow temperatures in liquid nitrogen. A procedure for cryopreservation of polyembryonic seeds was improved for selected citrus cultivars from Turkey. Seed dehydration was performed at different exposure times, in sterile conditions of a laminar flow-hood. The tested cultivars showed the highest tolerance to low temperature storage when the seeds were first dehydrated to a moisture content of 21.8% for Poncirus trifoliata Raf. × Citrus sinensis Osb. and to 17.6% for Citrus limonia Osbeck. The postcryopreservation germinability ranged from 73.3% (Poncirus trifoliata Raf. × C. sinensis Osb. and Fortunella margarita (Lour.) Swingle) to 93.3% (C. jambhiri Lush.). Dehydration was beneficial for germination of seeds from all of the tested citrus species after the liquid nitrogen exposure. Seedlings derived from cryopreserved seeds had well-formed shoots and roots and were easily acclimated to greenhouse conditions.
650 $aCryopreservation
650 $aCriopreservação
700 1 $aSOUZA, F. V. D.
700 1 $aGÖKDOGAN, E. Y.
700 1 $aCEYLAN, M.
700 1 $aJENDEREK, M. M.
773 $tTurkish Journal of Biology, 2017
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
