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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
25/03/2011 |
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Data da última atualização: |
15/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MOTA, G. B.; BATISTA, R. I. T. P.; SERAPIÃO, R. V.; BOITE, M. C.; VIANA, J. H. M. |
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Afiliação: |
Gustavo Bruno Mota, UFV; RIBRIO IVAN TAVARES PEREIRA BATISTA, UNIVERSIDADE ESTADUAL DO CEARÁ; RAQUEL VARELA SERAPIAO, PESAGRO - RIO; MARIANA CORTES BOITE, UFF; JOAO HENRIQUE MOREIRA VIANA, CNPGL. |
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Título: |
Developmental competence and expression of the MATER and ZAR1 genes in immature bovine oocytes selected by brilliant cresyl blue. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Zygote, v. 18, n. 3, p. 209-216, 2010. |
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DOI: |
https://doi.org/10.1017/S0967199409990219 |
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Idioma: |
Inglês |
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Conteúdo: |
The objective of this work was to evaluate the selection of immature bovine oocytes by brilliant cresyl blue dye (BCB) and expression of transcripts MATER and ZAR1. Cumulus–oocyte complexes (COCs) from slaughterhouse ovaries were exposed to BCB diluted in mDPBS and incubated for 60 min at 38.5 °C in humidified air. After exposure those COCs were distributed in two groups, according to their cytoplasm colour: BCB+ (coloured cytoplasm) or BCB− (colourless cytoplasm). The control group was submitted to in vitro maturation (IVM) immediately after morphological selection and holding control group COCs were exposed to mDPBS without BCB but in the same incubation conditions of BCB+ and BCB− group. The COCs of all groups were submitted to IVM, in vitro fertilization (IVF) and in vitro culture (IVC). Cleavage rate (72 h post-insemination) was similar between control (65.3%) and BCB+ (64.4%) groups, but greater than (p < 0.05) holding control (49.8%) and BCB− (51.3%) groups. Blastocyst rate (192 h post-insemination) was not different between BCB+ (18.5%) and control (16.3%) groups, but greater (p < 0.05) than BCB− (8.4%) group. No difference was found for blastocyst rate between holding control group (14.2%), control and BCB+ groups. The relative expression of MATER and ZAR1 genes was evaluated by real-time PCR in immature oocytes collected from the control, holding control, BCB+ and BCB− groups. Despite the relative expression of MATER in holding control, BCB+ and BCB− were down regulated in comparison to control group there was no statistical difference (p > 0.05) in the relative expression of MATER and ZAR1 transcripts among groups. The results indicate that the BCB dye detects immature oocyte populations with different developmental competence, although no improvement in in vitro embryo production using oocytes exposed or not to BCB was observed. Development competence of immature oocytes exposed to BCB does not seem to be associated with variations in the expression of MATER and ZAR1 transcripts. MenosThe objective of this work was to evaluate the selection of immature bovine oocytes by brilliant cresyl blue dye (BCB) and expression of transcripts MATER and ZAR1. Cumulus–oocyte complexes (COCs) from slaughterhouse ovaries were exposed to BCB diluted in mDPBS and incubated for 60 min at 38.5 °C in humidified air. After exposure those COCs were distributed in two groups, according to their cytoplasm colour: BCB+ (coloured cytoplasm) or BCB− (colourless cytoplasm). The control group was submitted to in vitro maturation (IVM) immediately after morphological selection and holding control group COCs were exposed to mDPBS without BCB but in the same incubation conditions of BCB+ and BCB− group. The COCs of all groups were submitted to IVM, in vitro fertilization (IVF) and in vitro culture (IVC). Cleavage rate (72 h post-insemination) was similar between control (65.3%) and BCB+ (64.4%) groups, but greater than (p < 0.05) holding control (49.8%) and BCB− (51.3%) groups. Blastocyst rate (192 h post-insemination) was not different between BCB+ (18.5%) and control (16.3%) groups, but greater (p < 0.05) than BCB− (8.4%) group. No difference was found for blastocyst rate between holding control group (14.2%), control and BCB+ groups. The relative expression of MATER and ZAR1 genes was evaluated by real-time PCR in immature oocytes collected from the control, holding control, BCB+ and BCB− groups. Despite the relative expression of MATER in holding control, BCB+ and BCB− were down regu... Mostrar Tudo |
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Palavras-Chave: |
Brilliant cresyl blue. |
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Thesaurus Nal: |
gene expression; in vitro fertilization; oocytes. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02786naa a2200229 a 4500
001 1883077
005 2024-02-15
008 2010 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1017/S0967199409990219$2DOI
100 1 $aMOTA, G. B.
245 $aDevelopmental competence and expression of the MATER and ZAR1 genes in immature bovine oocytes selected by brilliant cresyl blue.$h[electronic resource]
260 $c2010
520 $aThe objective of this work was to evaluate the selection of immature bovine oocytes by brilliant cresyl blue dye (BCB) and expression of transcripts MATER and ZAR1. Cumulus–oocyte complexes (COCs) from slaughterhouse ovaries were exposed to BCB diluted in mDPBS and incubated for 60 min at 38.5 °C in humidified air. After exposure those COCs were distributed in two groups, according to their cytoplasm colour: BCB+ (coloured cytoplasm) or BCB− (colourless cytoplasm). The control group was submitted to in vitro maturation (IVM) immediately after morphological selection and holding control group COCs were exposed to mDPBS without BCB but in the same incubation conditions of BCB+ and BCB− group. The COCs of all groups were submitted to IVM, in vitro fertilization (IVF) and in vitro culture (IVC). Cleavage rate (72 h post-insemination) was similar between control (65.3%) and BCB+ (64.4%) groups, but greater than (p < 0.05) holding control (49.8%) and BCB− (51.3%) groups. Blastocyst rate (192 h post-insemination) was not different between BCB+ (18.5%) and control (16.3%) groups, but greater (p < 0.05) than BCB− (8.4%) group. No difference was found for blastocyst rate between holding control group (14.2%), control and BCB+ groups. The relative expression of MATER and ZAR1 genes was evaluated by real-time PCR in immature oocytes collected from the control, holding control, BCB+ and BCB− groups. Despite the relative expression of MATER in holding control, BCB+ and BCB− were down regulated in comparison to control group there was no statistical difference (p > 0.05) in the relative expression of MATER and ZAR1 transcripts among groups. The results indicate that the BCB dye detects immature oocyte populations with different developmental competence, although no improvement in in vitro embryo production using oocytes exposed or not to BCB was observed. Development competence of immature oocytes exposed to BCB does not seem to be associated with variations in the expression of MATER and ZAR1 transcripts.
650 $agene expression
650 $ain vitro fertilization
650 $aoocytes
653 $aBrilliant cresyl blue
700 1 $aBATISTA, R. I. T. P.
700 1 $aSERAPIÃO, R. V.
700 1 $aBOITE, M. C.
700 1 $aVIANA, J. H. M.
773 $tZygote$gv. 18, n. 3, p. 209-216, 2010.
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Embrapa Gado de Leite (CNPGL) |
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| 1. |  | PERUFFO, R. G.; MOUSTACAS, V. S.; ECKSTEIN, C.; SANTOS, R. de L.; LOPES, L. B. Diagnóstico de brucella ovis pelo teste elisa usando o protocolo BP26r em ovinos do médio-norte mato-grossense. In: ENCONTRO DE CIÊNCIA E TECNOLOGIAS AGROSSUSTENTÁVEIS; JORNADA CIENTÍFICA DA EMBRAPA AGROSSILVIPASTORIL, 6., 2017, Sinop, MT. Resumos... Sinop, MT: Embrapa Agrossilpastoril, 2017. p. 132-135.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrossilvipastoril. |
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| 3. | | GUIMARÃES, A. G.; MOUSTACAS, V. S.; XAVIER, D. B.; MONTEIRO, R. A. C.; NASCIMENTO, A. F. do. Produção de leite de vacas mestiças em sistema silvipastoril. In: ENCONTRO DE CIÊNCIA E TECNOLOGIAS AGROSSUSTENTÁVEIS, 6.; JORNADA CIENTÍFICA DA EMBRAPA AGROSSILVIPASTORIL, 11., 2022. Sinop. Resumos... Brasília, DF: Embrapa, 2022. p. 34.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 4. | | GUIMARÃES, A. G.; MOUSTACAS, V. S.; XAVIER, D. B.; MONTEIRO, R. A. C.; NASCIMENTO, A. F. do. Produção de leite de vacas mestiças em sistema silvipastoril. In: ENCONTRO DE CIÊNCIA E TECNOLOGIAS AGROSSUSTENTÁVEIS, 6.; JORNADA CIENTÍFICA DA EMBRAPA AGROSSILVIPASTORIL, 11., 2022. Sinop. Resumos... Brasília, DF: Embrapa, 2022. p. 34.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrossilvipastoril. |
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| 5. | | LOPES, L. B.; MOUSTACAS, V. S.; ECKSTEIN, C.; D'ORNELLAS, M. P. F.; PONCE, H. L. dos S. Provas do antígeno acidificado tamponado e de reação em cadeia pela polimerase no diagnóstico da brucelose bovina em animais abatidos em frigorífico. In: FARIAS NETO, A. L. de; NASCIMENTO, A. F. do; ROSSONI, A. L.; MAGALHÃES, C. A. de S.; ITUASSU, D. R.; HOOGERHEIDE, E. S. S.; IKEDA, F. S.; FERNANDES JUNIOR, F.; FARIA, G. R.; ISERNHAGEN, I.; VENDRUSCULO, L. G.; MORALES, M. M.; CARNEVALLI, R. A. (Ed.). Embrapa Agrossilvipastoril: primeiras contribuições para o desenvolvimento de uma agropecuária sustentável. Brasília, DF: Embrapa, 2019. pt. 5, cap. 3, p. 375-379.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Agrossilvipastoril. |
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| 7. | | SILVA, A. B. da; MONTEIRO, R. A. C.; CARVALHO, C. A. B. de; MORENZ, D. A.; MOUSTACAS, V. S. Atividade de pastejo de vacas leiteiras em sistemas silvipastoris. In: ENCONTRO DE CIÊNCIA E TECNOLOGIAS AGROSSUSTENTÁVEIS, 2.; JORNADA CIENTÍFICA DA EMBRAPA AGROSSILVIPASTORIL, 7., 2018. Sinop, MT. Resumos... Sinop, MT: Embrapa Agrossilpastoril, 2018. p. 88-91.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrossilvipastoril. |
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| 9. | | SILVA, A. B. da; MONTEIRO, R. A. C.; CARVALHO, C. A. B. de; MORENZ, D. A.; MOUSTACAS, V. S.; XAVIER, D. B.; ABATTI, G.; XAVIER, M. L. M. Sombra para vacas mestiças leiteiras em sistemas silvipastoris. In: SIMPÓSIO INTERNACIONAL SOBRE SUSTENTABILIDADE DA BOVINOCULTURA DE LEITE E CORTE NO CERRADO, 2019, Campo Grande. Perspectivas e desafios para a produção de leite e carne no Cerrado do Mato Grosso do Sul. Campo Grande, MS: FAMEZ: UFMS, 2019.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 10. | | VARAGO, F. C.; SILVA, L. P.; RIBEIRO, J. R.; FERNANDES, C. A.; CARVALHO, B. C. de; GIOSO, M. M.; MOUSTACAS, V. S. Teofilina como agente capacitante do sêmen bovino. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, v. 69, n. 6, p. 1607-1614, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 11. | | VARAGO, F. C.; SILVA, L. P.; RIBEIRO, J. R.; FERNANDES, C. A.; CARVALHO, B. C. de; GIOSO, M. M.; MOUSTACAS, V. S. Teofilina como agente capacitante do sêmen bovino. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, v. 69, n. 6, p. 1607-1614, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Agrossilvipastoril. |
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| 12. | | ECKSTEIN, C.; BENTO, F. C.; MOURÃO, R. C.; LOPES, L. B.; GOMES, H. F. B.; MOUSTACAS, V. S.; SANTOS, R. de L. Characterization of the sheep industry in the medium northern region of Mato Grosso, Brazil. Revista brasileira de ciência veterinária, v. 24, n. 2, p. 81-85, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
| Biblioteca(s): Embrapa Agrossilvipastoril. |
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| 13. | | ECKSTEIN, C.; MOUSTACAS, V. S.; LOPES, L. B.; MOL, J. P. da S.; GOMES, S. C.; SANTOS, R. dos; CASTRO, B. G. de; SANTOS, R. L. Differential diagnosis of infectious reproductive diseases in sheep flocks of Mato Grosso State, Brazil. Small Ruminant Research, v. 153, p. 158-162, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Agrossilvipastoril. |
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| 15. | | MORENZ, A. B. da S.; CARVALHO, C. A. B. de; MONTEIRO, R. A. C.; MORENZ, D. A.; BARROS, I. de; LULU, J.; MOUSTACAS, V. S.; XAVIER, D. B. Dairy cows on integrated livestock-forestry system in the tropics. Agroforestry Systems, v. 98, p. 1079-1090, 2024.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Gado de Leite; Embrapa Soja. |
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| 16. | | SILVA, A. B. da; MONTEIRO, R. A. C.; CARVALHO, C. H. B. de; MORENZ, D. A.; MOUSTACAS, V. S.; XAVIER, D. B.; RODRIGUES, K. da S.; FAVERO, E. B. Atividade de ruminação de vacas leiteiras em sistemas silvipastoris. In: SIMPÓSIO INTERNACIONAL SOBRE SUSTENTABILIDADE DA BOVINOCULTURA DE LEITE E CORTE NO CERRADO, 2019, Campo Grande. Perspectivas e desafios para a produção de leite e carne no Cerrado do Mato Grosso do Sul. Campo Grande, MS: FAMEZ: UFMS, 2019.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 17. | | SILVA, A. B. da; MONTEIRO, R. A. C.; CARVALHO, C. A. B. de; MONREZ, D. A.; MOUSTACAS, V. S.; XAVIER, D. B.; OLIVEIRA, C. F. P. de; MIGUEL, B. L. N. Atividade de pastejo de vacas leiteiras em sistemas silvipastoris. In: SIMPÓSIO INTERNACIONAL SOBRE SUSTENTABILIDADE DA BOVINOCULTURA DE LEITE E CORTE NO CERRADO, 2019, Campo Grande. Perspectivas e desafios para a produção de leite e carne no Cerrado do Mato Grosso do Sul. Campo Grande, MS: FAMEZ: UFMS, 2019.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 18. | | PACKER, A. T. M.; SILVA, A. P. M. da; BETTI, A. de F. F.; SILVA, B. R. da; SILVA, F. P.; MAGALHAES, J. C.; PINTO, J. M. A.; CHELEGAO, R.; BICUDO, R. de C.; MOUSTACAS, V. S. Setor de gestão de laboratórios (SGL). In: FARIAS NETO, A. L. de; NASCIMENTO, A. F. do; ROSSONI, A. L.; MAGALHÃES, C. A. de S.; ITUASSU, D. R.; HOOGERHEIDE, E. S. S.; IKEDA, F. S.; FERNANDES JUNIOR, F.; FARIA, G. R.; ISERNHAGEN, I.; VENDRUSCULO, L. G.; MORALES, M. M.; CARNEVALLI, R. A. (Ed.). Embrapa Agrossilvipastoril: primeiras contribuições para o desenvolvimento de uma agropecuária sustentável. Brasília, DF: Embrapa, 2019. pt. 11, cap. 8, p. 809-823.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Agrossilvipastoril. |
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| 19. | | SILVA, A. B. da; MONTEIRO, R. A. C.; CARVALHO, C. A. B. de; BARROS, I. de; MORENZ, D. A.; MOUSTACAS, V. S.; XAVIER, D. B.; RODRIGUES, K. da S. Pasture shadowing reduces searches for water troughs by dairy cows. In: WORLD CONGRESS ON INTEGRATED CROP-LIVESTOCK-FORESTRY SYSTEMS, 2., 2021. Proceedings reference. Brasília, DF: Embrapa, 2021. p. 181-185.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrossilvipastoril. |
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| 20. | | SILVA, A. B. da; MONTEIRO, R. A. C.; CARVALHO, C. A. B. de; BARROS, I. de; MORENZ, D. A.; MOUSTACAS, V. S.; XAVIER, D. B.; RODRIGUES, K. da S. Pasture shadowing reduces searches for water troughs by dairy cows. In: WORLD CONGRESS ON INTEGRATED CROP-LIVESTOCK-FORESTRY SYSTEMS, 2., 2021. Proceedings reference. Brasília, DF: Embrapa, 2021. p. 181-185.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| Registros recuperados : 20 | |
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