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Registro Completo |
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Biblioteca(s): |
Embrapa Amazônia Ocidental. |
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Data corrente: |
24/06/2015 |
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Data da última atualização: |
03/03/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MEHRABI, R.; GOHARI, A. M.; SILVA, G. F. da; STEINBERG, G.; KEMA, G. H. J.; WIT, P. J. G. M. de. |
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Afiliação: |
Rahim Mehrabi, Cereal Research Department; Amir Mirzadi Gohari, Plant Research International B.V.; GILVAN FERREIRA DA SILVA, CPAA; Gero Steinberg, University of Exeter; Gert H. J. Kema, Plant Research International B.V.; Pierre J. G. M. de Wit, Wageningen University. |
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Título: |
Flexible gateway constructs for functional analyses of genes in plant pathogenic fungi. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Fungal Genetics and Biology, v. 79, p. 186-192, 2015. |
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DOI: |
http://dx.doi.org/10.1016/j.fgb.2015.03.016 |
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Idioma: |
Inglês |
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Conteúdo: |
Genetic manipulation of fungi requires quick, low-cost, efficient, high-throughput and molecular tools. In this paper, we report 22 entry constructs as new molecular tools based on the Gateway technology facilitating rapid construction of binary vectors that can be used for functional analysis of genes in fungi. The entry vectors for single, double or triple gene-deletion mutants were developed using hygromycin, geneticin and nourseothricin resistance genes as selection markers. Furthermore, entry vectors containing green fluorescent (GFP) or red fluorescent (RFP) in combination with hygromycin, geneticin or nour- seothricin selection markers were generated. The latter vectors provide the possibility of gene deletion and simultaneous labelling of the fungal transformants with GFP or RFP reporter genes. The applicability of a number of entry vectors was validated in Zymoseptoria tritici, an important fungal wheat pathogen |
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Palavras-Chave: |
Gateway technology; Zymoseptoria tritici. |
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Thesaurus Nal: |
gene deletion. |
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Categoria do assunto: |
-- |
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Marc: |
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245 $aFlexible gateway constructs for functional analyses of genes in plant pathogenic fungi.$h[electronic resource]
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520 $aGenetic manipulation of fungi requires quick, low-cost, efficient, high-throughput and molecular tools. In this paper, we report 22 entry constructs as new molecular tools based on the Gateway technology facilitating rapid construction of binary vectors that can be used for functional analysis of genes in fungi. The entry vectors for single, double or triple gene-deletion mutants were developed using hygromycin, geneticin and nourseothricin resistance genes as selection markers. Furthermore, entry vectors containing green fluorescent (GFP) or red fluorescent (RFP) in combination with hygromycin, geneticin or nour- seothricin selection markers were generated. The latter vectors provide the possibility of gene deletion and simultaneous labelling of the fungal transformants with GFP or RFP reporter genes. The applicability of a number of entry vectors was validated in Zymoseptoria tritici, an important fungal wheat pathogen
650 $agene deletion
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773 $tFungal Genetics and Biology$gv. 79, p. 186-192, 2015.
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Registro original: |
Embrapa Amazônia Ocidental (CPAA) |
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