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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
11/07/2025 |
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Data da última atualização: |
14/07/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OLIVEIRA, M. S. de; SILVA, N. P. da; PAULA, M. S. de; PORTO, D. L.; ARAGAO, C. F. S.; SEGURA, M. E. C.; FABRI, R. L.; BEMQUERER, M. P.; BRANDAO, H. de M.; TAVARES, G. D.; APOLONIO, A. C. M. |
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Afiliação: |
MARIANA SIMOES DE OLIVEIRA, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; NATALIA PRADO DA SILVA, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; MARCELLE SILVA ALVES DE PAULA, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; DAYANNE LOPES PORTO, UNIVERSIDADE FEDERAL DO RIO GRANDE DO NORTE; CÍCERO FLAVIO SOARES ARAGAO, UNIVERSIDADE FEDERAL DO RIO GRANDE DO NORTE; MARIA ESPERANZA CORTES SEGURA, UNIVERSIDADE FEDERAL DE MINAS GERAIS; RODRIGO LUIZ FABRI, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; MARCELO PORTO BEMQUERER, CNPGL; HUMBERTO DE MELLO BRANDAO, CNPGL; GUILHERME DINIZ TAVARES, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; ANA CAROLINA MORAIS APOLONIO, UNIVERSIDADE FEDERAL DE JUIZ DE FORA. |
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Título: |
Poly(ε-caprolactone) nanocapsules coated with Chitosan optimize the antimicrobial activity of Tea Tree Oil and Azithromycin against oral pathogens. |
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Ano de publicação: |
2025 |
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Fonte/Imprenta: |
Microbial Pathogenesis, v. 206, 107820, 2025. |
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DOI: |
https://doi.org/10.1016/j.micpath.2025.107820 |
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Idioma: |
Português |
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Conteúdo: |
This study aimed to develop poly(ε-caprolactone) nanocapsules coated with chitosan, loaded with Tea Tree Oil (TTO) and Azithromycin (AZI), to optimize the antimicrobial activity and bioavailability of these actives. The nanocapsules suspension (NP-TTO-AZI-CH) was prepared using the preformed polymer interfacial precipitation method and coated with a 2 % chitosan solution. Physicochemical characterization included evaluating the pH, mean hydrodynamic diameter (Z-Average), Zeta potential (ZP), polydispersity index (PdI), encapsulation efficiency (EE) of the essential oil, and morphological assessment by Scanning Electron Microscopy (SEM) and Atomic Force Microscopy (AFM). Biological tests conducted encompassed Minimum Inhibitory Concentration (MIC), Minimum Microbicidal Concentration (MMC), and cell viability assessments. NP-TTO-AZI-CH showed a ZAverage of 238.5 ± 1.7 nm and a PdI of 0.245 ± 0.010, indicating homogeneity in particle size and distribution. The positive ZP (+28.06 ± 1.10 mV) suggested the presence of chitosan on the nanocapsule surfaces, making the suspension more acidic (pH 4.38). EE was high at 89.89 ± 1.29 %. SEM images revealed the spherical/oval shape of the nanocapsules, and AFM confirmed their nanometric size and distribution within the system. Encapsulated TTO and AZI demonstrated lower MIC values compared to their free forms for all tested strains within 24 h, indicating enhanced antimicrobial efficacy. NP-TTO-AZI-CH exhibited bactericidal activity and maintained 70 % cell viability within the first 24 h at concentrations ranging from 0.5 mg/mL + 20 μg/mL to 0.06 mg/mL + 2.5 μg/mL (TTO + AZI). This formulation proved to be a promising tool for enhancing the antimicrobial activity of bioactives and combating various oral microbial strains. MenosThis study aimed to develop poly(ε-caprolactone) nanocapsules coated with chitosan, loaded with Tea Tree Oil (TTO) and Azithromycin (AZI), to optimize the antimicrobial activity and bioavailability of these actives. The nanocapsules suspension (NP-TTO-AZI-CH) was prepared using the preformed polymer interfacial precipitation method and coated with a 2 % chitosan solution. Physicochemical characterization included evaluating the pH, mean hydrodynamic diameter (Z-Average), Zeta potential (ZP), polydispersity index (PdI), encapsulation efficiency (EE) of the essential oil, and morphological assessment by Scanning Electron Microscopy (SEM) and Atomic Force Microscopy (AFM). Biological tests conducted encompassed Minimum Inhibitory Concentration (MIC), Minimum Microbicidal Concentration (MMC), and cell viability assessments. NP-TTO-AZI-CH showed a ZAverage of 238.5 ± 1.7 nm and a PdI of 0.245 ± 0.010, indicating homogeneity in particle size and distribution. The positive ZP (+28.06 ± 1.10 mV) suggested the presence of chitosan on the nanocapsule surfaces, making the suspension more acidic (pH 4.38). EE was high at 89.89 ± 1.29 %. SEM images revealed the spherical/oval shape of the nanocapsules, and AFM confirmed their nanometric size and distribution within the system. Encapsulated TTO and AZI demonstrated lower MIC values compared to their free forms for all tested strains within 24 h, indicating enhanced antimicrobial efficacy. NP-TTO-AZI-CH exhibited bactericidal activity and ... Mostrar Tudo |
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Palavras-Chave: |
Nanotecnologia; Quitosana. |
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Thesagro: |
Óleo Essencial. |
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Thesaurus Nal: |
Anti-infective agents; Essential oils; Nanoparticles; Nanotechnology. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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| Registros recuperados : 5 | |
| 1. |  | RAMALHO, B.; DIECKOW, J.; MELO, V. de F.; BROWN, G. G.; SIMON, P. L.; IBARR, M. A.; CUNHA, L.; KILLE, P.; TPI NETWORK. Carbon and nitrogen storage and stability by mineral-organic association in physical fractions of anthropogenic dark earth and of reference soils in Amazonia. Catena, v. 213, 106185, June 2022. 10 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Florestas. |
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| 2. | | MELO, T. O.; FRANCISCON, L.; BROWN, G. G.; KOPKA, J.; CUNHA, L.; MARTINEZ-SEIDEL, F.; MADUREIRA, L. A. dos S.; HANSEL, F. A.; TPI Network. Univariate statistical analysis of gaschromatography: mass spectrometry fingerprints analyses. Chemical Date Collections, v. 33, Id 100719, 2021. Date article.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 5 |
| Biblioteca(s): Embrapa Florestas. |
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| 3. | | CUNHA, L.; BARTZ, M.; DEMETRIO, W.; SILVA, T.; JAMES, S.; SILVA, E. da; STANTON, D.; CONRADO, A. C.; DECAENS, T.; LAVELLE, P.; SANTOS, A.; NADOLNY, H.; VELÁSQUEZ, E.; ZANGERLÉ, A.; TAPIA-CORAL, S.; FERREIRA, T.; MAIA, L.; SEGALLA, R.; CLEMENT, C.; MUNIZ, A. W.; KILLE, P.; BROWN, G. G.; TPI NETWORK. Earthworms and Amazonian Dark Earths: improving understanding of the relationships between soil management, biodiversity and function. In: INTERNATIONAL OLIGOCHAETE TAXONOMY MEETING, 7., 2016, Paimpont. Taxonomy, phygeny and ecology of earthworm's communities. [Rennes]: Université de Rennes, [2016]. Não paginado.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Florestas. |
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| 4. | | SÁTIRO, J. N. de O.; MOTTA, A. C. V.; DEMETRIO, W. C.; SEGALLA, R. F.; CREMONESI, M. V.; ARAÚJO, E. M.; FALCÃO, N. P. de S.; MARTINS, G. C.; MUNIZ, A. W.; TAUBE, P. S.; REBELLATO, L.; OLIVEIRA JUNIOR, R. C. de; TEIXEIRA, W. G.; NEVES, E. G.; LIMA, H. P.; SHOCK, M. P.; KILLE, P.; CUNHA, L.; TPI NETWORK; BROWN, G. G. Micronutrient availability in amazonian dark earths and adjacent soils. Geoderma, v. 395, Art. 115072, Aug. 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Amazônia Ocidental; Embrapa Amazônia Oriental; Embrapa Florestas; Embrapa Solos. |
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| 5. | | CONRADO, A. C.; DEMETRIO, W. C.; STANTON, D. W. G.; BARTZ, M. L. C.; JAMES, S. W.; SANTOS, A.; SILVA, E. da; FERREIRA, T.; ACIOLI, A. N. S.; FERREIRA, A. C.; MAIA, L. S.; SILVA, T. A. C.; LAVELLE, P.; VELASQUEZ, E.; TAPIA-CORAL, S. C.; MUNIZ, A. W.; SEGALLA, R. F.; DECAËNS, T.; NADOLNY, H. S.; PEÑA-VENEGAS, C.; PASINI, A.; OLIVEIRA JUNIOR, R. C. de; TPI NETWORK; KILLE, P.; BROWN, G. G.; CUNHA, L. Amazonian earthworm biodiversity is heavily impacted by ancient and recent human disturbance. Science of the Total Environment, v. 895, art. 165087, 2023.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Amazônia Ocidental; Embrapa Florestas. |
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| Registros recuperados : 5 | |
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