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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
16/11/2010 |
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Data da última atualização: |
10/12/2010 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ZEID, M.; YU, J. K.; GOLDOWITZ, I.; DENTON, M. E.; COSTICH, D. E.; JAYASURIYA, C. T.; SAHA, M.; ELSHIRE, R.; BENSCHER, D.; BRESEGHELLO, F.; MUNKVOLD, J.; VARSHNEY, R. K.; BELAY, G.; SORRELLS, M. E. |
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Afiliação: |
M. ZEID, CORNELL UNIVERSITY; J. K. YU, CORNELL UNIVERSITY; I. GOLDOWITZ, CORNELL UNIVERSITY; M. E. DENTON, CORNELL UNIVERSITY; DENISE E. COSTICH, CORNELL UNIVERSITY; C. T. JAYASURIYA, CORNELL UNIVERSITY; M. SAHA, THE SAMUEL ROBERTS NOBLE FOUNDATION; R. ELSHIRE, CORNELL UNIVERSITY; D. BENSCHER, CORNELL UNIVERSITY; FLAVIO BRESEGHELLO, CNPAF; J. MUNKVOLD, BOYCE THOMPSON INSTITUTE; R. K. VARSHNEY, ICRISAT; G. BELAY, ETHIOPIAN INSTITUTE OF AGRICULTURAL RESEARCH; M. E. SORRELLS, CORNELL UNIVERSITY. |
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Título: |
Cross-amplification of EST-derived markers among 16 grass species. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Field Crops Research, Amsterdam, v. 118, n. 1, p. 28-35, Jul. 2010. |
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Idioma: |
Inglês |
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Conteúdo: |
The availability of a large number of expressed sequence tags (ESTs) has facilitated the development of molecular markers in members of the grass family. As these markers are derived from coding sequences, cross-species amplification and transferability is higher than for markers designed from genomic DNA sequences. In this study, 919 EST-based primers developed from seven grass species were assessed for their amplification across a diverse panel of 16 grass species including cereal, turf and forage crops. Out of the 919 primers tested, 89 successfully amplified DNA from one or more species and 340 primers generated PCR amplicons from at least half of the species in the panel. Only 5.2% of the primers tested produced clear amplicons in all 16 species. The majority of the primers (66.9%) were developed from tall fescue and rice and these two species showed amplification rate of 41.6% and 19.0% across the panel, respectively. The highest amplification rate was found for conserved-intron scanning primers (CISP) developed from pearl millet (91%) and sorghum (75%) EST sequences that aligned to rice sequences. The primers with successful amplification identified in this study showed promise in other grass species as demonstrated in differentiating a set of 13 clones of reed canary grass, a species for which very little genomic research has been done. Sequences from the amplified PCR fragments indicated the potential for the transferable CISP markers for comparative mapping purposes. These primer sets can be immediately used for within and across species mapping and will be especially useful for minor grass species with few or no available molecular markers. MenosThe availability of a large number of expressed sequence tags (ESTs) has facilitated the development of molecular markers in members of the grass family. As these markers are derived from coding sequences, cross-species amplification and transferability is higher than for markers designed from genomic DNA sequences. In this study, 919 EST-based primers developed from seven grass species were assessed for their amplification across a diverse panel of 16 grass species including cereal, turf and forage crops. Out of the 919 primers tested, 89 successfully amplified DNA from one or more species and 340 primers generated PCR amplicons from at least half of the species in the panel. Only 5.2% of the primers tested produced clear amplicons in all 16 species. The majority of the primers (66.9%) were developed from tall fescue and rice and these two species showed amplification rate of 41.6% and 19.0% across the panel, respectively. The highest amplification rate was found for conserved-intron scanning primers (CISP) developed from pearl millet (91%) and sorghum (75%) EST sequences that aligned to rice sequences. The primers with successful amplification identified in this study showed promise in other grass species as demonstrated in differentiating a set of 13 clones of reed canary grass, a species for which very little genomic research has been done. Sequences from the amplified PCR fragments indicated the potential for the transferable CISP markers for comparative mapping purpose... Mostrar Tudo |
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Palavras-Chave: |
CISP; EST-SSR. |
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Thesagro: |
Genética molecular; Gramínea; Marcador molecular. |
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Thesaurus Nal: |
Poaceae; Sequence homology. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02615naa a2200361 a 4500
001 1867036
005 2010-12-10
008 2010 bl uuuu u00u1 u #d
100 1 $aZEID, M.
245 $aCross-amplification of EST-derived markers among 16 grass species.
260 $c2010
520 $aThe availability of a large number of expressed sequence tags (ESTs) has facilitated the development of molecular markers in members of the grass family. As these markers are derived from coding sequences, cross-species amplification and transferability is higher than for markers designed from genomic DNA sequences. In this study, 919 EST-based primers developed from seven grass species were assessed for their amplification across a diverse panel of 16 grass species including cereal, turf and forage crops. Out of the 919 primers tested, 89 successfully amplified DNA from one or more species and 340 primers generated PCR amplicons from at least half of the species in the panel. Only 5.2% of the primers tested produced clear amplicons in all 16 species. The majority of the primers (66.9%) were developed from tall fescue and rice and these two species showed amplification rate of 41.6% and 19.0% across the panel, respectively. The highest amplification rate was found for conserved-intron scanning primers (CISP) developed from pearl millet (91%) and sorghum (75%) EST sequences that aligned to rice sequences. The primers with successful amplification identified in this study showed promise in other grass species as demonstrated in differentiating a set of 13 clones of reed canary grass, a species for which very little genomic research has been done. Sequences from the amplified PCR fragments indicated the potential for the transferable CISP markers for comparative mapping purposes. These primer sets can be immediately used for within and across species mapping and will be especially useful for minor grass species with few or no available molecular markers.
650 $aPoaceae
650 $aSequence homology
650 $aGenética molecular
650 $aGramínea
650 $aMarcador molecular
653 $aCISP
653 $aEST-SSR
700 1 $aYU, J. K.
700 1 $aGOLDOWITZ, I.
700 1 $aDENTON, M. E.
700 1 $aCOSTICH, D. E.
700 1 $aJAYASURIYA, C. T.
700 1 $aSAHA, M.
700 1 $aELSHIRE, R.
700 1 $aBENSCHER, D.
700 1 $aBRESEGHELLO, F.
700 1 $aMUNKVOLD, J.
700 1 $aVARSHNEY, R. K.
700 1 $aBELAY, G.
700 1 $aSORRELLS, M. E.
773 $tField Crops Research, Amsterdam$gv. 118, n. 1, p. 28-35, Jul. 2010.
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