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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
23/12/2004 |
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Data da última atualização: |
30/05/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MAGALHAES, J. R.; HUBER, D. M. |
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Afiliação: |
EMBRAPA-CNPMS. |
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Título: |
Free ammonia, free amino acids, and enzyme activity in maize tissue treated with methionine sulfoximine. |
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Ano de publicação: |
1991 |
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Fonte/Imprenta: |
Journal of Plant Nutrition, New York, v. 14, n. 8, p. 883-895, 1991. |
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Idioma: |
Inglês |
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Conteúdo: |
Glutamine synthetase and glutamte dehydrogenase activity, free ammonia, and changes in free amino acid pools in tissue of three maize genotypes differing in enzyme activity were measured after O, 2, 6, 12, and 24 hour incubation with 1 mM methionine sulfoximine (MSX) and 10 mM NH4+ in nutrient solution. Glutamine synthetase activity was almost nil in roots and decreased about 80 percent in shoots after six hr incubation with MSX. Glutamate dehydrogenase activity was not affected by MSX, but was very low in tissue of the -GDH maize mutant. In general, total amino acids decreased with time in MSX. Glutamate in root tissues of the Illiinois N6 wild type decreased 80 percent after 24 hr incubation with MSX while it was reduced only 60 percent in the Illinois N6 mutant. |
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Palavras-Chave: |
Glutamina. |
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Thesagro: |
Milho. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 01274naa a2200157 a 4500
001 1488575
005 2018-05-30
008 1991 bl uuuu u00u1 u #d
100 1 $aMAGALHAES, J. R.
245 $aFree ammonia, free amino acids, and enzyme activity in maize tissue treated with methionine sulfoximine.$h[electronic resource]
260 $c1991
520 $aGlutamine synthetase and glutamte dehydrogenase activity, free ammonia, and changes in free amino acid pools in tissue of three maize genotypes differing in enzyme activity were measured after O, 2, 6, 12, and 24 hour incubation with 1 mM methionine sulfoximine (MSX) and 10 mM NH4+ in nutrient solution. Glutamine synthetase activity was almost nil in roots and decreased about 80 percent in shoots after six hr incubation with MSX. Glutamate dehydrogenase activity was not affected by MSX, but was very low in tissue of the -GDH maize mutant. In general, total amino acids decreased with time in MSX. Glutamate in root tissues of the Illiinois N6 wild type decreased 80 percent after 24 hr incubation with MSX while it was reduced only 60 percent in the Illinois N6 mutant.
650 $aMilho
653 $aGlutamina
700 1 $aHUBER, D. M.
773 $tJournal of Plant Nutrition, New York$gv. 14, n. 8, p. 883-895, 1991.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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