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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
09/01/2013 |
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Data da última atualização: |
25/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CIDADE, L. C.; OLIVEIRA, T. M. de; MENDES, A. F. S.; MACEDO, A. F.; FLOH, E. I. S.; GESTEIRA, A. da S.; SOARES FILHO, W. dos S.; COSTA, M. G. C. |
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Afiliação: |
LUCIANA C. CIDADE, UESC; TAHISE M. DE OLIVEIRA, UESC; AMANDA F. S. MENDES, UESC; AMANDA F. MACEDO, USP; ENY I. S. FLOH, USP; ABELMON DA SILVA GESTEIRA, CNPMF; WALTER DOS SANTOS SOARES FILHO, CNPMF; MARCIO G. C. COSTA, UESC. |
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Título: |
Ectopic expression of a fruit phytoene synthase from Citrus paradisi Macf. promotes abiotic stress tolerance in transgenic tobacco. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Molecular Biology Report, v.39, p. 10201-10209, 2012 |
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ISSN: |
0301-4851 |
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DOI: |
10.1007/s11033-012-1895-2 |
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Idioma: |
Inglês |
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Conteúdo: |
Abscisic acid (ABA) is an important regulator of plant responses to environmental stresses and an absolute requirement for stress tolerance. Recently, a third phytoene synthase (PSY3) gene paralog was identified in monocots and demonstrated to play a specialized role in stress-induced ABA formation, thus suggesting that the first committed step in carotenogenesis is a key limiting step in ABA biosynthesis. To examine whether the ectopic expression of PSY, other than PSY3, would similarly affect ABA level and stress tolerance, we have produced transgenic tobacco containing a fruit-specific PSY (CpPSY) of grapefruit (Citrus paradisi Macf.). The transgenic plants contained a single- or double-locus insertion and expressed CpPSY at varying transcript levels. In comparison with the wild-type plants, the CpPSY expressing transgenic plants showed a significant increase on root length and shoot biomass under PEG-, NaCl- and mannitol-induced osmotic stress. The enhanced stress tolerance of transgenic plants was correlated with the increased endogenous ABA level and expression of stress-responsive genes, which in turn was correlated with the CpPSY copy number and expression level in different transgenic lines. Collectively, these results provide further evidence that PSY is a key enzyme regulating ABA biosynthesis and that the altered expression of other PSYs in transgenic plants may provide a similar function to that of the monocot?s PSY3 in ABA biosynthesis and stress tolerance. The results also pave the way for further use of CpPSY, as well as other PSYs, as potential candidate genes for engineering tolerance to drought and salt stress in crop plants. MenosAbscisic acid (ABA) is an important regulator of plant responses to environmental stresses and an absolute requirement for stress tolerance. Recently, a third phytoene synthase (PSY3) gene paralog was identified in monocots and demonstrated to play a specialized role in stress-induced ABA formation, thus suggesting that the first committed step in carotenogenesis is a key limiting step in ABA biosynthesis. To examine whether the ectopic expression of PSY, other than PSY3, would similarly affect ABA level and stress tolerance, we have produced transgenic tobacco containing a fruit-specific PSY (CpPSY) of grapefruit (Citrus paradisi Macf.). The transgenic plants contained a single- or double-locus insertion and expressed CpPSY at varying transcript levels. In comparison with the wild-type plants, the CpPSY expressing transgenic plants showed a significant increase on root length and shoot biomass under PEG-, NaCl- and mannitol-induced osmotic stress. The enhanced stress tolerance of transgenic plants was correlated with the increased endogenous ABA level and expression of stress-responsive genes, which in turn was correlated with the CpPSY copy number and expression level in different transgenic lines. Collectively, these results provide further evidence that PSY is a key enzyme regulating ABA biosynthesis and that the altered expression of other PSYs in transgenic plants may provide a similar function to that of the monocot?s PSY3 in ABA biosynthesis and stress tolerance. The... Mostrar Tudo |
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Thesaurus Nal: |
Citrus; tobacco. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
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008 2012 bl uuuu u00u1 u #d
022 $a0301-4851
024 7 $a10.1007/s11033-012-1895-2$2DOI
100 1 $aCIDADE, L. C.
245 $aEctopic expression of a fruit phytoene synthase from Citrus paradisi Macf. promotes abiotic stress tolerance in transgenic tobacco.$h[electronic resource]
260 $c2012
520 $aAbscisic acid (ABA) is an important regulator of plant responses to environmental stresses and an absolute requirement for stress tolerance. Recently, a third phytoene synthase (PSY3) gene paralog was identified in monocots and demonstrated to play a specialized role in stress-induced ABA formation, thus suggesting that the first committed step in carotenogenesis is a key limiting step in ABA biosynthesis. To examine whether the ectopic expression of PSY, other than PSY3, would similarly affect ABA level and stress tolerance, we have produced transgenic tobacco containing a fruit-specific PSY (CpPSY) of grapefruit (Citrus paradisi Macf.). The transgenic plants contained a single- or double-locus insertion and expressed CpPSY at varying transcript levels. In comparison with the wild-type plants, the CpPSY expressing transgenic plants showed a significant increase on root length and shoot biomass under PEG-, NaCl- and mannitol-induced osmotic stress. The enhanced stress tolerance of transgenic plants was correlated with the increased endogenous ABA level and expression of stress-responsive genes, which in turn was correlated with the CpPSY copy number and expression level in different transgenic lines. Collectively, these results provide further evidence that PSY is a key enzyme regulating ABA biosynthesis and that the altered expression of other PSYs in transgenic plants may provide a similar function to that of the monocot?s PSY3 in ABA biosynthesis and stress tolerance. The results also pave the way for further use of CpPSY, as well as other PSYs, as potential candidate genes for engineering tolerance to drought and salt stress in crop plants.
650 $aCitrus
650 $atobacco
700 1 $aOLIVEIRA, T. M. de
700 1 $aMENDES, A. F. S.
700 1 $aMACEDO, A. F.
700 1 $aFLOH, E. I. S.
700 1 $aGESTEIRA, A. da S.
700 1 $aSOARES FILHO, W. dos S.
700 1 $aCOSTA, M. G. C.
773 $tMolecular Biology Report$gv.39, p. 10201-10209, 2012
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| Registros recuperados : 7 | |
| 3. |  | OLIVEIRA, R. J. P. de; VINHAS, P.; DOUMER, M. E.; ANTUNES, L. E. C.; CAMPOS, A. D. Quantificacão de acúcares e amido em ramos de mirtilo coletados para estaquia em três diferentes épocas no período de dormência da cultura. In: CONGRESSO DE INICIAÇÃO CIENTÍFICA, 2.; ENCONTRO DE PÓS GRADUAÇÃO DA EMBRAPA CLIMA TEMPERADO, 2., 2008, Pelotas. anais... Pelotas: Embrapa Clima Temperado, 2008. p. 117-121 Volume 2.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 4. | | OLIVEIRA, R. J. P. de; VINHAS, P.; DOUMER, M.; MATTOS, G. S. de; CAMPOS, A. D.; ANTUNES, L. E. C. Investigação da presença de Triptofano em ramos lenhosos de mirtilo. In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 20.; ANNUAL MEETING OF THE INTERAMERICAN SOCIETY FOR TROPICAL HORTICULTURE, 54., 2008, Vitória. Anais... Frutas para todos: estratégias, tecnologias e visão sustentável. Vitória: Sociedade Brasileira de Fruticultura, 2008. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 5. | | DOUMER, M.; MESSIAS, R.; SILVA, S. D. dos A. e.; PAGEL, I.; ARAÚJO, V.; PILLON, C. N. Teor de óleo em duas cultivares de girassol (Helianthus annuus L.) tratadas com formulações de fertilizantes foliares. In: SIMPÓSIO ESTADUAL DE AGROENERGIA, 1.; REUNIÃO TÉCNICA ANUAL DE AGROENERGIA-RS, 1., 2007, Pelotas. Anais... Pelotas: Embrapa Clima Temperado, 2007. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 6. | | GARDIN, J. P. P.; DOUMER, M. E.; MESSIAS, R. da S.; FERREIRA, L. H. G.; SILVEIRA, C. A. P.; PILLON, C. N. Avaliação do efeito de fertilizantes foliares a base de água de xisto na produtividade e na atividade enzimática na cultura do milho. In: CONGRESSO BRASILEIRO DE ROCHAGEM, 1., 2010, Brasilia. Anais... Brasília, Petrobras/Embrapa, 2010.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 7. | | PIRES, E. da S.; MITTELMANN, A.; CASTRO, R. L. de; GABE, N. L.; DOUMER, M. E.; AMARAL, R. P. do; GABANA, A. Distribuição da produção de forragem em populações de azevém na região da fronteira oeste do rio grande do sul. In: CONGRESSO DE INICIAÇÃO CIENTIFICA, 18.; ENCONTRO DE PÓS GRADUAÇÃO, 11., E MOSTRA CIENTIFICA, 1., 2009, Pelotas. Anais... Pelotas: UFPel, 2009. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
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| Registros recuperados : 7 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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