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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
03/10/2011 |
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Data da última atualização: |
05/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BRITO, M. A. V. P. e; SOMKUTI, G. A.; RENYE JUNIOR, J. A. |
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Afiliação: |
MARIA APARECIDA V PAIVA E BRITO, CNPGL; G. A. SOMKUTI, Eastern Regional Research Center, U. S. Departmento of Agriculture; J. A. RENYE JUNIOR, Eastern Regional Research Center, U. S. Departmento of Agriculture. |
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Título: |
Production of antilisterial bacteriocins by staphylococci isolated from bovine milk. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Journal of Dairy Science, v. 94, p. 1194-1200, 2011. |
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DOI: |
https://doi.org/10.3168/jds.2010-3849 |
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Idioma: |
Inglês |
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Conteúdo: |
A collection of 111 staphylococcal isolates recovered from healthy cows in 41 dairy herds in Brazil was surveyed for the production of bacteriocins. The group included 94 coagulase-positive and 17 coagulase-negative strains of staphylococci. All cultures were grown in tryptic soy broth for 18h at 37°C, and cell-free supernatants were tested for antimicrobial activity against several target organisms by using the agar diffusion method. Filtrates of 57 staphylococci showed strong activity against Listeria monocytogenes Scott A, and 52 isolates also inhibited the growth of Stapylococcus aureus Newbould 305, a major causative agent of bovine mastitis in the United States. The plasmid profiles of staphylococci invariably included an 8-kb plasmid. Staphylococcal isolates were tested for the production of aureocins A70 and A53, 2 bacteriocins of coagulase-positive staphylococci known to be associated with 8-kb and 10.2-kb plasmids, respectively. The presence of the A70 or A53 bacteriocin gene was checked by PCR techniques using primers based on nucleotide sequences flanking the structural gene of each bacteriocin. Agarose gel analysis of amplified PCR products of plasmid templates from all 58 isolates showed only a 525-bp fragment corresponding to the structural gene of the bacteriocin aureocin A70. The results indicated that the apparently widespread association of A70-producing staphylococci with healthy cows in Brazil may be beneficial in controlling undesirable bacteria in dairy herds. MenosA collection of 111 staphylococcal isolates recovered from healthy cows in 41 dairy herds in Brazil was surveyed for the production of bacteriocins. The group included 94 coagulase-positive and 17 coagulase-negative strains of staphylococci. All cultures were grown in tryptic soy broth for 18h at 37°C, and cell-free supernatants were tested for antimicrobial activity against several target organisms by using the agar diffusion method. Filtrates of 57 staphylococci showed strong activity against Listeria monocytogenes Scott A, and 52 isolates also inhibited the growth of Stapylococcus aureus Newbould 305, a major causative agent of bovine mastitis in the United States. The plasmid profiles of staphylococci invariably included an 8-kb plasmid. Staphylococcal isolates were tested for the production of aureocins A70 and A53, 2 bacteriocins of coagulase-positive staphylococci known to be associated with 8-kb and 10.2-kb plasmids, respectively. The presence of the A70 or A53 bacteriocin gene was checked by PCR techniques using primers based on nucleotide sequences flanking the structural gene of each bacteriocin. Agarose gel analysis of amplified PCR products of plasmid templates from all 58 isolates showed only a 525-bp fragment corresponding to the structural gene of the bacteriocin aureocin A70. The results indicated that the apparently widespread association of A70-producing staphylococci with healthy cows in Brazil may be beneficial in controlling undesirable bacteria in dair... Mostrar Tudo |
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Palavras-Chave: |
Antilisterial activity; Bacteriocin; Staphylococci from milk. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02131naa a2200193 a 4500
001 1902017
005 2024-02-05
008 2011 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3168/jds.2010-3849$2DOI
100 1 $aBRITO, M. A. V. P. e
245 $aProduction of antilisterial bacteriocins by staphylococci isolated from bovine milk.$h[electronic resource]
260 $c2011
520 $aA collection of 111 staphylococcal isolates recovered from healthy cows in 41 dairy herds in Brazil was surveyed for the production of bacteriocins. The group included 94 coagulase-positive and 17 coagulase-negative strains of staphylococci. All cultures were grown in tryptic soy broth for 18h at 37°C, and cell-free supernatants were tested for antimicrobial activity against several target organisms by using the agar diffusion method. Filtrates of 57 staphylococci showed strong activity against Listeria monocytogenes Scott A, and 52 isolates also inhibited the growth of Stapylococcus aureus Newbould 305, a major causative agent of bovine mastitis in the United States. The plasmid profiles of staphylococci invariably included an 8-kb plasmid. Staphylococcal isolates were tested for the production of aureocins A70 and A53, 2 bacteriocins of coagulase-positive staphylococci known to be associated with 8-kb and 10.2-kb plasmids, respectively. The presence of the A70 or A53 bacteriocin gene was checked by PCR techniques using primers based on nucleotide sequences flanking the structural gene of each bacteriocin. Agarose gel analysis of amplified PCR products of plasmid templates from all 58 isolates showed only a 525-bp fragment corresponding to the structural gene of the bacteriocin aureocin A70. The results indicated that the apparently widespread association of A70-producing staphylococci with healthy cows in Brazil may be beneficial in controlling undesirable bacteria in dairy herds.
653 $aAntilisterial activity
653 $aBacteriocin
653 $aStaphylococci from milk
700 1 $aSOMKUTI, G. A.
700 1 $aRENYE JUNIOR, J. A.
773 $tJournal of Dairy Science$gv. 94, p. 1194-1200, 2011.
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Embrapa Gado de Leite (CNPGL) |
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| Registros recuperados : 6 | |
| 1. |  | LAVORATO, J. A. A.; PINTO, M. A. DE O.; OTENIO, M. H.; PINTO, C. L. DE O.; BRUMANO, L. P.; NEVES, K. C. DE L. Diretrizes para elaboração de manual de boas praticas de laboratório para indústrias de laticínios de pequeno e médio porte com base na representação social dos utiliazadores. In: CONGRESSO NACIONAL DE LATICÍNIOS, 29., 2012, Juiz de Fora. Anais... Belo Horizonte: EPAMIG, 2012.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 2. | | BESSA, M. E. De; RODARTE, M. P.; OTENIO, M. H.; STRINGHETA, P. C.; OLIVEIRA, J. M. De; BARBOSA, J. C.; PINTO, M. A. De O. Sensory perception of the fermented goat milk: potential application of the DSC method. Food Science and Technology, v. 36, n. 3, p. 406-412, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 3. | | MEURER, I. R.; LANGE, C. C.; HUNGARO, H. M.; BELL, M. J. V.; ANJOS, V. de C. dos; SILVA, C. A. de S.; PINTO, M. A. de O. Quantification of whole ultra high temperature UHT milk waste as a function of packages type and design. Journal of Cleaner Production, v. 153, p. 483-490, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 4. | | ASSIS, A. S. F.; CRUZ, L. T.; OTENIO, M. H.; PINTO, M. A. DE O.; BESSA, M. A.; VIEIRA, C. B.; MIAGOSTOVICH, M. P.; ROSA E SILVA, M. L. Environmental surveillance of rotavirus in drainage basin of Juiz de Fora, MG and its relation with the microbiological and physico-chemical parameters. In: SIMPOSIO LATINOAMERICANO DE VIROLOGÍA AMBIENTAL, 2., 2013, Salto, Uruguay. Resumos... Salto: Universidade de la República; Fiocruz, 2013.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 5. | | ASSIS, A. S. F.; CRUZ, L. T.; PINTO, M. A. DE O.; BESSA, M. A.; DRUMOND, B. P.; OTENIO, M. H.; VIEIRA, C. B.; MIAGOSTOVICH, M. P.; ROSA E SILVA, M. L. Relationship between microbiological and physico-chemical parameters and occurrence of rotavirus genomes in drainage basin of Juiz de Fora, MG. Journal of the Brazilian Society for Virology, v. 17, n. 2, supl. 1, p. 117-118, 2012. Edição dos abstracts do 23º Brazilian Congress of Virology e 7º Mercosur Meeting of Virology, Foz do Iguaçu, 2012.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 6. | | ASSIS, A. S.; CRUZ, L. T.; FERREIRA, A. S.; BESSA, M. E.; PINTO, M. A. de O.; VIEIRA, C. B.; OTENIO, M. H.; MIAGOSTOVICH, M. P.; SILVA, M. L. da R. e. Relationship between viral detection and turbidity in a watershed contaminated with group A rotavirus. Environmental Science and Pollution Research, v. 22, p. 6886-6897, 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| Registros recuperados : 6 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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