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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
11/12/2000 |
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Data da última atualização: |
10/08/2023 |
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Autoria: |
BEDIDI-MADANI, N.; KODJO, A.; VILLARD, L.; RICHARD, Y. |
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Título: |
Ribotyping of Staphylococcus caprae isolated from goat milk. |
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Ano de publicação: |
1998 |
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Fonte/Imprenta: |
Veterinary Research, v. 29, n. 2, p. 149-158, 1998. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: The usefulness of the API-STAPH system and a method based on ribosomal fingerprinting was evaluated by studying 89 coagulase-negative staphylococci isolated from goat milk. The bacteria were supposed to belong to the species Staphylococcus caprae. The results obtained from the API-STAPH system showed variations in their phenotypic features. DNA cleavage with EcoRI yielded six ribotypes and three distinct patterns were generated when the DNA of the strains was digested with HindIII. Forty-six strains were correctly characterized as S. caprae by the two methods. In addition, 37 isolates having atypical biochemical profiles with the API-STAPH system were confirmed as being S. caprae by the ribotyping. Three strains remained unclassified by both methods. Ribotypes generated by HindIII were found to be the most informative for species determination, whereas the number of bands generated by EcoRI indicated the usefulness of this enzyme in S. caprae typing. |
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Palavras-Chave: |
Mastite. |
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Thesagro: |
Caprino; Etiologia. |
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Thesaurus Nal: |
Females; Food microbiology; Goat milk; Goats; Restriction mapping; Ribosomal DNA; Staphylococcus caprae. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 01702naa a2200277 a 4500
001 1524199
005 2023-08-10
008 1998 bl uuuu u00u1 u #d
100 1 $aBEDIDI-MADANI, N.
245 $aRibotyping of Staphylococcus caprae isolated from goat milk.$h[electronic resource]
260 $c1998
520 $aAbstract: The usefulness of the API-STAPH system and a method based on ribosomal fingerprinting was evaluated by studying 89 coagulase-negative staphylococci isolated from goat milk. The bacteria were supposed to belong to the species Staphylococcus caprae. The results obtained from the API-STAPH system showed variations in their phenotypic features. DNA cleavage with EcoRI yielded six ribotypes and three distinct patterns were generated when the DNA of the strains was digested with HindIII. Forty-six strains were correctly characterized as S. caprae by the two methods. In addition, 37 isolates having atypical biochemical profiles with the API-STAPH system were confirmed as being S. caprae by the ribotyping. Three strains remained unclassified by both methods. Ribotypes generated by HindIII were found to be the most informative for species determination, whereas the number of bands generated by EcoRI indicated the usefulness of this enzyme in S. caprae typing.
650 $aFemales
650 $aFood microbiology
650 $aGoat milk
650 $aGoats
650 $aRestriction mapping
650 $aRibosomal DNA
650 $aStaphylococcus caprae
650 $aCaprino
650 $aEtiologia
653 $aMastite
700 1 $aKODJO, A.
700 1 $aVILLARD, L.
700 1 $aRICHARD, Y.
773 $tVeterinary Research$gv. 29, n. 2, p. 149-158, 1998.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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