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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
19/09/2000 |
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Data da última atualização: |
08/06/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PURCINO, A. A. C.; SILVA, M. R. e; ANDRADE, S. R. M.; BELELE, C. L.; PARENTONI, S. N.; SANTOS, M. X. dos. |
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Afiliação: |
ANTONIO ALVARO CORSETTI PURCINO, CNPMS; SIDNEY NETTO PARENTONI, CNPMS. |
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Título: |
Grain filling in maize: the effect of nitrogen nutrition on the activities of nitrogen assimilating enzymes in the pedicel-placento-chalaza region. |
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Ano de publicação: |
2000 |
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Fonte/Imprenta: |
Maydica, Bergamo, v. 45, n. 2, p. 95-103, 2000. |
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Idioma: |
Inglês |
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Conteúdo: |
In a developing maize kernel, the pedicel-placento-chalaza (PPCh) is a heterogeneous tissue between the cob vascular system and the endosperm. Its major role is to modify the cob vascular system amino acid pool composition to better suit the requirements of the developing endosperm. Nitrogen metabolism in the PPCh tissue may, therefore, influence individual kernel development and consequently play an important role in determining grain yield capacity in maize. The objectives of the two experiments reported in this work were: (1) to determine the effect of nitrogen supply on the activities of the nitrogen assimilating enzymes glutamine synthetase (GS), NADH-dependent glutamate synthase (NADH-GOGAT), glutamate dehydrogenase (GDH) and of phospho-enolpyruvate carboxylase (PEPC) in the pedicel-placentochalaza (PPCh) tissue of maize genotypes contrasting in their responsiveness to nitrogen (N) fertilization, and (2) to verify if the activities of these enzymes in the PPCh tissue are correlated to individual kernel weight. Hybrids were grown in the field under low (16 kg/ha) and high (116 kg/ha) N supply and hand pollinated at silking. Enzymes activities were measured 30 days after pollination (DAP) and individual kernel weight was determined after black layer formation. Nitrogen supply did not influence individual kernel weight, soluble protein, GS and PEPC activities. However inconsistent results were observed for NADH-GOGAT and GDH over experimental years. Enzyme activities varied among genotypes, but correlation determinations indicated that individual kernel weight was not associated with soluble protein content and GS activity, with discrepant results observed for NADH-GOGAAT and GDH over years. However, a positive and significant correlation was observed between kernel weight and PEPC activity in both experiments. This finding merits further investigations over a larger number of genotypes and different environmental conditions. It suggests that genotypes with increased PEPC activity in the PPCh tissue have increased kernel weight and that this enzyme plays an important role in determining grain yield in maize. MenosIn a developing maize kernel, the pedicel-placento-chalaza (PPCh) is a heterogeneous tissue between the cob vascular system and the endosperm. Its major role is to modify the cob vascular system amino acid pool composition to better suit the requirements of the developing endosperm. Nitrogen metabolism in the PPCh tissue may, therefore, influence individual kernel development and consequently play an important role in determining grain yield capacity in maize. The objectives of the two experiments reported in this work were: (1) to determine the effect of nitrogen supply on the activities of the nitrogen assimilating enzymes glutamine synthetase (GS), NADH-dependent glutamate synthase (NADH-GOGAT), glutamate dehydrogenase (GDH) and of phospho-enolpyruvate carboxylase (PEPC) in the pedicel-placentochalaza (PPCh) tissue of maize genotypes contrasting in their responsiveness to nitrogen (N) fertilization, and (2) to verify if the activities of these enzymes in the PPCh tissue are correlated to individual kernel weight. Hybrids were grown in the field under low (16 kg/ha) and high (116 kg/ha) N supply and hand pollinated at silking. Enzymes activities were measured 30 days after pollination (DAP) and individual kernel weight was determined after black layer formation. Nitrogen supply did not influence individual kernel weight, soluble protein, GS and PEPC activities. However inconsistent results were observed for NADH-GOGAT and GDH over experimental years. Enzyme activities vari... Mostrar Tudo |
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Palavras-Chave: |
Efeito; Effects; Enchimento; Grain filling; Graos; Maize. |
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Thesagro: |
Fisiologia; Milho; Nitrogênio; Zea Mays. |
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Thesaurus Nal: |
nitrogen; physiology. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 03057naa a2200325 a 4500
001 1484157
005 2018-06-08
008 2000 bl uuuu u00u1 u #d
100 1 $aPURCINO, A. A. C.
245 $aGrain filling in maize$bthe effect of nitrogen nutrition on the activities of nitrogen assimilating enzymes in the pedicel-placento-chalaza region.$h[electronic resource]
260 $c2000
520 $aIn a developing maize kernel, the pedicel-placento-chalaza (PPCh) is a heterogeneous tissue between the cob vascular system and the endosperm. Its major role is to modify the cob vascular system amino acid pool composition to better suit the requirements of the developing endosperm. Nitrogen metabolism in the PPCh tissue may, therefore, influence individual kernel development and consequently play an important role in determining grain yield capacity in maize. The objectives of the two experiments reported in this work were: (1) to determine the effect of nitrogen supply on the activities of the nitrogen assimilating enzymes glutamine synthetase (GS), NADH-dependent glutamate synthase (NADH-GOGAT), glutamate dehydrogenase (GDH) and of phospho-enolpyruvate carboxylase (PEPC) in the pedicel-placentochalaza (PPCh) tissue of maize genotypes contrasting in their responsiveness to nitrogen (N) fertilization, and (2) to verify if the activities of these enzymes in the PPCh tissue are correlated to individual kernel weight. Hybrids were grown in the field under low (16 kg/ha) and high (116 kg/ha) N supply and hand pollinated at silking. Enzymes activities were measured 30 days after pollination (DAP) and individual kernel weight was determined after black layer formation. Nitrogen supply did not influence individual kernel weight, soluble protein, GS and PEPC activities. However inconsistent results were observed for NADH-GOGAT and GDH over experimental years. Enzyme activities varied among genotypes, but correlation determinations indicated that individual kernel weight was not associated with soluble protein content and GS activity, with discrepant results observed for NADH-GOGAAT and GDH over years. However, a positive and significant correlation was observed between kernel weight and PEPC activity in both experiments. This finding merits further investigations over a larger number of genotypes and different environmental conditions. It suggests that genotypes with increased PEPC activity in the PPCh tissue have increased kernel weight and that this enzyme plays an important role in determining grain yield in maize.
650 $anitrogen
650 $aphysiology
650 $aFisiologia
650 $aMilho
650 $aNitrogênio
650 $aZea Mays
653 $aEfeito
653 $aEffects
653 $aEnchimento
653 $aGrain filling
653 $aGraos
653 $aMaize
700 1 $aSILVA, M. R. e
700 1 $aANDRADE, S. R. M.
700 1 $aBELELE, C. L.
700 1 $aPARENTONI, S. N.
700 1 $aSANTOS, M. X. dos
773 $tMaydica, Bergamo$gv. 45, n. 2, p. 95-103, 2000.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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Biblioteca |
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Tipo/Formato |
Classificação |
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Registro |
Volume |
Status |
URL |
Voltar
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| Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
12/03/2008 |
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Data da última atualização: |
15/05/2024 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
CUNHA, N. B.; LIMA, G. R.; CIPRIANO, T. M.; LEITE, A.; ARAGÃO, F. J. L.; VIANNA, G. R.; RECH, E. L. |
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Afiliação: |
NICOLAU BRITO DA CUNHA FILHO; G. R. LIMA, UNIVERSIDADE DE BRASÍLIA; THAÍS DE MOURA CIPRIANO; A. LEITE, UNIVERSIDADE DE CAMPINAS; FRANCISCO JOSE LIMA ARAGÃO, EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA; GIOVANNI RODRIGUES VIANNA, EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA; ELIBIO LEOPOLDO RECH FILHO, EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA. |
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Título: |
Expressão do gene do hormônio do crescimento humano (hGH) em plantas transgênicas de soja [Glycine max L. (MERRIL)]. |
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Ano de publicação: |
2007 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE GENÉTICA, 53., 2007, Águas de Lindóia. Resumos... Águas de Lindóia: Sociedade Brasileira de Genética, 2007. |
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Páginas: |
p. 228 |
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Idioma: |
Português |
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Palavras-Chave: |
Biorreatores; Expressão de biomoléculas; Heterologous protein; human growth hormone; Plant farming; Seed specific expression; Seed storage protein; Transgenic plantas; Transgenic soybean. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00970nam a2200289 a 4500
001 1180946
005 2024-05-15
008 2007 bl uuuu u01u1 u #d
100 1 $aCUNHA, N. B.
245 $aExpressão do gene do hormônio do crescimento humano (hGH) em plantas transgênicas de soja [Glycine max L. (MERRIL)].
260 $aIn: CONGRESSO BRASILEIRO DE GENÉTICA, 53., 2007, Águas de Lindóia. Resumos... Águas de Lindóia: Sociedade Brasileira de Genética$c2007
300 $ap. 228
653 $aBiorreatores
653 $aExpressão de biomoléculas
653 $aHeterologous protein
653 $ahuman growth hormone
653 $aPlant farming
653 $aSeed specific expression
653 $aSeed storage protein
653 $aTransgenic plantas
653 $aTransgenic soybean
700 1 $aLIMA, G. R.
700 1 $aCIPRIANO, T. M.
700 1 $aLEITE, A.
700 1 $aARAGÃO, F. J. L.
700 1 $aVIANNA, G. R.
700 1 $aRECH, E. L.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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