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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
22/08/2025 |
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Data da última atualização: |
22/08/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
DOCEMA, M. L.; MORAES, T. de S.; ATTÍLIO, L. B.; PIEDADE, S. M. de S.; MARQUES, J. P. R.; STIPP, L. C. L.; HARAKAVA, R.; ASTUA, J. de F.; MOURÃO FILHO, F. de A. A. |
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Afiliação: |
MATHEUS LUÍS DOCEMA, UNIVERSIDADE DE SÃO PAULO; TATIANA DE SOUZA MORAES, UNIVERSIDADE DE SÃO PAULO; LÍSIA BORGES ATTÍLIO, UNIVERSIDADE DE SÃO PAULO; SÔNIA MARIA DE STEFANO PIEDADE, UNIVERSIDADE DE SÃO PAULO; JOÃO PAULO RODRIGUES MARQUES, UNIVERSIDADE DE SÃO PAULO; LILIANE CRISTINA LIBORIO STIPP, UNIVERSIDADE DE SÃO PAULO; RICARDO HARAKAVA, INSTITUTO BIOLÓGICO; JULIANA DE FREITAS ASTUA, CNPMF; FRANCISCO DE ASSIS ALVES MOURÃO FILHO, UNIVERSIDADE DE SÃO PAULO. |
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Título: |
Reaction of transgenic ‘Hamlin’ sweet orange expressing. |
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Ano de publicação: |
2025 |
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Fonte/Imprenta: |
The Journal of Horticultural Science and Biotechnology, p.1-13, 2025 . |
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ISSN: |
0022-1589 |
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DOI: |
https://doi.org/10.1080/14620316.2025.2515958 |
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Idioma: |
Inglês |
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Conteúdo: |
ABSTRACT We evaluated the response of ‘Hamlin’ sweet orange plants (Citrus x sinensis L. Osbeck) transformed with the gene encoding the synthetic antimicrobial peptide d4e1, driven by the 35S promoter, or with the gene encoding copper-zinc superoxide dismutase (csd1), isolated from Poncirus trifoliata and driven by the Ubiquitin 10 promoter, against Candidatus Liberibacter asiaticus (CLas). The experimental plants were propagated by grafting and then inoculated with CLas-infected budwood. The presence and concentration of CLas were determined in leaf tissues by qPCR six, 12, and 18 months after inoculation (m.a.i.). Callose detection was evaluated in transgenic sweet orange events after pathogen inoculation through epifluorescence analysis. Five transgenic events expressing the csd1 gene and one expressing the d4e1 gene exhibited reduced CLas populations compared to non-transgenic plants and showed no visible symptoms of huanglongbing (HLB), six m.a.i. Moreover, an increase in callose deposition in phloem tissues of some transgenic events may be a defence response to biotic stresses, leading to lower CLas populations. |
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Thesagro: |
Laranja. |
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Categoria do assunto: |
-- |
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Marc: |
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022 $a0022-1589
024 7 $ahttps://doi.org/10.1080/14620316.2025.2515958$2DOI
100 1 $aDOCEMA, M. L.
245 $aReaction of transgenic ‘Hamlin’ sweet orange expressing.$h[electronic resource]
260 $c2025
520 $aABSTRACT We evaluated the response of ‘Hamlin’ sweet orange plants (Citrus x sinensis L. Osbeck) transformed with the gene encoding the synthetic antimicrobial peptide d4e1, driven by the 35S promoter, or with the gene encoding copper-zinc superoxide dismutase (csd1), isolated from Poncirus trifoliata and driven by the Ubiquitin 10 promoter, against Candidatus Liberibacter asiaticus (CLas). The experimental plants were propagated by grafting and then inoculated with CLas-infected budwood. The presence and concentration of CLas were determined in leaf tissues by qPCR six, 12, and 18 months after inoculation (m.a.i.). Callose detection was evaluated in transgenic sweet orange events after pathogen inoculation through epifluorescence analysis. Five transgenic events expressing the csd1 gene and one expressing the d4e1 gene exhibited reduced CLas populations compared to non-transgenic plants and showed no visible symptoms of huanglongbing (HLB), six m.a.i. Moreover, an increase in callose deposition in phloem tissues of some transgenic events may be a defence response to biotic stresses, leading to lower CLas populations.
650 $aLaranja
700 1 $aMORAES, T. de S.
700 1 $aATTÍLIO, L. B.
700 1 $aPIEDADE, S. M. de S.
700 1 $aMARQUES, J. P. R.
700 1 $aSTIPP, L. C. L.
700 1 $aHARAKAVA, R.
700 1 $aASTUA, J. de F.
700 1 $aMOURÃO FILHO, F. de A. A.
773 $tThe Journal of Horticultural Science and Biotechnology, p.1-13, 2025 .
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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