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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
25/07/2024 |
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Data da última atualização: |
25/07/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PAIVA, B. A. R.; PREVEAUX, A.; DARRASSE, A.; WENDLAND, A.; ROSSATO, M.; MARQUES, A. S. dos A.; FERREIRA, M. A S. V.; JACQUES, M.-A. |
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Afiliação: |
BRUNA ALÍCIA R. PAIVA, UNIVERSIDADE DE BRASÍLIA; ANNE PREVEAUX, UNIVERSITÉ ANGERS; ARMELLE DARRASSE, UNIVERSITÉ ANGERS; ADRIANE WENDLAND FERREIRA, CNPAF; MAURÍCIO ROSSATO, UNIVERSIDADE DE BRASÍLIA; ABI SOARES DOS ANJOS MARQUES, CENARGEN; MARISA A. S. V. FERREIRA, UNIVERSIDADE DE BRASÍLIA; MARIE‑AGNÈS JACQUES, UNIVERSITÉ ANGERS. |
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Título: |
A survey of common bacterial blight in Central Brazil reveals a third Xanthomonas species infecting common bean. |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
Tropical Plant Pathology, v. 49, n. 4, p. 566-572, Aug. 2024. |
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ISSN: |
1983-2052 |
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DOI: |
https://doi.org/10.1007/s40858-024-00655-3 |
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Idioma: |
Inglês |
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Conteúdo: |
A survey of common bacterial blight (CBB) was conducted in common bean fields in Brazil, in the state of Goiás and in the Federal District. Isolation from symptomatic leaves on a semi-selective medium yielded yellow, mucoid colonies typical of Xanthomonas, and 161 pure cultures were obtained. PCR with specific primers for X. phaseoli pv. phaseoli and X. citri pv. fuscans was performed and confirmed the presence of both fuscans and GL2 strains of X. citri pv. fuscans and the presence of X. phaseoli pv. phaseoli. However, for 81 isolates, PCR results were negative. Housekeeping genes gyrB and rpoD sequences placed these strains in the provisionally named X. cannabis species clade, clustering pv. zinniae, pv. esculenti, and the strain Nyagatare, isolated from beans in Rwanda in 2013. The identification of a subset of 20 strains was complemented by a positive PCR with Xanthomonas-specific primers that amplify a portion of the gumD gene and the induction of hypersensitive reaction in tomato leaves within 24-48 hours. Pathogenicity was confirmed by inoculation on the French common bean cv. Flavert and the Brazilian common bean cv. BRS Ártico. Symptoms such as white spots along the leaf blade, leaf curling, and wilting developed in inoculated plants. Partial gyrB and rpoD sequences analyses revealed identity values ranging from 98.3 to 100% between the Brazilian isolates and strain Nyagatare, proposed as X. cannabis pv. phaseoli. |
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Palavras-Chave: |
Distrito Federal; Goiás; Multilocus sequence analysis; Strain Nyagatare; Xanthomonads. |
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Thesagro: |
Doença de Planta; Feijão; Mancha Bacteriana; Phaseolus Vulgaris; Xanthomonas Phaseoli. |
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Thesaurus Nal: |
Bacterial diseases of plants; Beans; Diseases and disorders (animals and humans); Multilocus sequence typing. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02678naa a2200397 a 4500
001 2165975
005 2024-07-25
008 2024 bl uuuu u00u1 u #d
022 $a1983-2052
024 7 $ahttps://doi.org/10.1007/s40858-024-00655-3$2DOI
100 1 $aPAIVA, B. A. R.
245 $aA survey of common bacterial blight in Central Brazil reveals a third Xanthomonas species infecting common bean.$h[electronic resource]
260 $c2024
520 $aA survey of common bacterial blight (CBB) was conducted in common bean fields in Brazil, in the state of Goiás and in the Federal District. Isolation from symptomatic leaves on a semi-selective medium yielded yellow, mucoid colonies typical of Xanthomonas, and 161 pure cultures were obtained. PCR with specific primers for X. phaseoli pv. phaseoli and X. citri pv. fuscans was performed and confirmed the presence of both fuscans and GL2 strains of X. citri pv. fuscans and the presence of X. phaseoli pv. phaseoli. However, for 81 isolates, PCR results were negative. Housekeeping genes gyrB and rpoD sequences placed these strains in the provisionally named X. cannabis species clade, clustering pv. zinniae, pv. esculenti, and the strain Nyagatare, isolated from beans in Rwanda in 2013. The identification of a subset of 20 strains was complemented by a positive PCR with Xanthomonas-specific primers that amplify a portion of the gumD gene and the induction of hypersensitive reaction in tomato leaves within 24-48 hours. Pathogenicity was confirmed by inoculation on the French common bean cv. Flavert and the Brazilian common bean cv. BRS Ártico. Symptoms such as white spots along the leaf blade, leaf curling, and wilting developed in inoculated plants. Partial gyrB and rpoD sequences analyses revealed identity values ranging from 98.3 to 100% between the Brazilian isolates and strain Nyagatare, proposed as X. cannabis pv. phaseoli.
650 $aBacterial diseases of plants
650 $aBeans
650 $aDiseases and disorders (animals and humans)
650 $aMultilocus sequence typing
650 $aDoença de Planta
650 $aFeijão
650 $aMancha Bacteriana
650 $aPhaseolus Vulgaris
650 $aXanthomonas Phaseoli
653 $aDistrito Federal
653 $aGoiás
653 $aMultilocus sequence analysis
653 $aStrain Nyagatare
653 $aXanthomonads
700 1 $aPREVEAUX, A.
700 1 $aDARRASSE, A.
700 1 $aWENDLAND, A.
700 1 $aROSSATO, M.
700 1 $aMARQUES, A. S. dos A.
700 1 $aFERREIRA, M. A S. V.
700 1 $aJACQUES, M.-A.
773 $tTropical Plant Pathology$gv. 49, n. 4, p. 566-572, Aug. 2024.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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