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 | Acesso ao texto completo restrito à biblioteca da Embrapa Amazônia Oriental. Para informações adicionais entre em contato com cpatu.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Amazônia Oriental; Embrapa Gado de Leite. |
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Data corrente: |
14/12/2020 |
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Data da última atualização: |
14/12/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FIGUEIRO, M. R.; GARCIA, J. M.; LIMA, M. R. de; COLLADO, M. del; SARAIVA, N. Z. |
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Afiliação: |
MARIVALDO RODRIGUES FIGUEIRO, CPATU; Joaquim Mansano Garcia, UNESP; Marina Ragagnin de Lima, UNESP; Maite del Collado, USP; NAIARA ZOCCAL SARAIVA, CNPGL. |
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Título: |
Is it possible to alter the embryo lipid accumulation with reduction of fetal bovine serum and use of l-carnitine for in vitro maturation of bubaline oocytes? |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Zygote, v. 28, n. 2, p. 109-115, Apr. 2020. |
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DOI: |
https://doi.org/10.1017/S096719941900073X |
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Idioma: |
Inglês |
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Conteúdo: |
In vitro embryo production (IVEP) is a procedure that can promote genetic improvement in a short time frame. However, the success rates obtained with this biotechnology in water buffaloes are still inconsistent, and can be associated with the high concentration of lipids in the cytoplasm of oocytes and embryos. The objective of this study was to evaluate the effects of reduced concentration of fetal bovine serum (FBS) and/or use of l-carnitine during in vitro maturation (IVM) on the preimplantation development and lipid accumulation in bubaline embryos. In a first experiment, the lowest concentration of FBS in the IVM medium (0%, 2.5%, 5% or 10%) was determined, and the lowest concentration that maintained good embryo development rates was 5%. In a second experiment, the addition of 5 mM of l-carnitine into the maturation medium was evaluated. The blastocysts produced were submitted to lipid evaluation involving staining followed by observation using optical (Oil Red O) and confocal (BODIPY 493/503) microscopy. No difference was observed between the 5% and 10% FBS groups, which were superior to the 0% and 2.5% groups. Furthermore, the performance of the groups treated with 5% and 10% FBS was better than the groups supplemented with l-carnitine. There was no difference regarding embryo lipid accumulation. The results indicated that it is possible to reduce the FBS concentration to 5% in in vitro maturation medium for production of bubaline embryos, and supplementation with 5 mM l-carnitine does not increase embryo production. MenosIn vitro embryo production (IVEP) is a procedure that can promote genetic improvement in a short time frame. However, the success rates obtained with this biotechnology in water buffaloes are still inconsistent, and can be associated with the high concentration of lipids in the cytoplasm of oocytes and embryos. The objective of this study was to evaluate the effects of reduced concentration of fetal bovine serum (FBS) and/or use of l-carnitine during in vitro maturation (IVM) on the preimplantation development and lipid accumulation in bubaline embryos. In a first experiment, the lowest concentration of FBS in the IVM medium (0%, 2.5%, 5% or 10%) was determined, and the lowest concentration that maintained good embryo development rates was 5%. In a second experiment, the addition of 5 mM of l-carnitine into the maturation medium was evaluated. The blastocysts produced were submitted to lipid evaluation involving staining followed by observation using optical (Oil Red O) and confocal (BODIPY 493/503) microscopy. No difference was observed between the 5% and 10% FBS groups, which were superior to the 0% and 2.5% groups. Furthermore, the performance of the groups treated with 5% and 10% FBS was better than the groups supplemented with l-carnitine. There was no difference regarding embryo lipid accumulation. The results indicated that it is possible to reduce the FBS concentration to 5% in in vitro maturation medium for production of bubaline embryos, and supplementation with 5 ... Mostrar Tudo |
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Palavras-Chave: |
Desenvolvimento embrionário; Soro fetal bovino. |
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Thesagro: |
Búfalo; Lipídio. |
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Thesaurus Nal: |
Buffaloes; Lipid metabolism. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02374naa a2200253 a 4500
001 2128094
005 2020-12-14
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1017/S096719941900073X$2DOI
100 1 $aFIGUEIRO, M. R.
245 $aIs it possible to alter the embryo lipid accumulation with reduction of fetal bovine serum and use of l-carnitine for in vitro maturation of bubaline oocytes?$h[electronic resource]
260 $c2020
520 $aIn vitro embryo production (IVEP) is a procedure that can promote genetic improvement in a short time frame. However, the success rates obtained with this biotechnology in water buffaloes are still inconsistent, and can be associated with the high concentration of lipids in the cytoplasm of oocytes and embryos. The objective of this study was to evaluate the effects of reduced concentration of fetal bovine serum (FBS) and/or use of l-carnitine during in vitro maturation (IVM) on the preimplantation development and lipid accumulation in bubaline embryos. In a first experiment, the lowest concentration of FBS in the IVM medium (0%, 2.5%, 5% or 10%) was determined, and the lowest concentration that maintained good embryo development rates was 5%. In a second experiment, the addition of 5 mM of l-carnitine into the maturation medium was evaluated. The blastocysts produced were submitted to lipid evaluation involving staining followed by observation using optical (Oil Red O) and confocal (BODIPY 493/503) microscopy. No difference was observed between the 5% and 10% FBS groups, which were superior to the 0% and 2.5% groups. Furthermore, the performance of the groups treated with 5% and 10% FBS was better than the groups supplemented with l-carnitine. There was no difference regarding embryo lipid accumulation. The results indicated that it is possible to reduce the FBS concentration to 5% in in vitro maturation medium for production of bubaline embryos, and supplementation with 5 mM l-carnitine does not increase embryo production.
650 $aBuffaloes
650 $aLipid metabolism
650 $aBúfalo
650 $aLipídio
653 $aDesenvolvimento embrionário
653 $aSoro fetal bovino
700 1 $aGARCIA, J. M.
700 1 $aLIMA, M. R. de
700 1 $aCOLLADO, M. del
700 1 $aSARAIVA, N. Z.
773 $tZygote$gv. 28, n. 2, p. 109-115, Apr. 2020.
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Registro original: |
Embrapa Amazônia Oriental (CPATU) |
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| Registros recuperados : 137 | |
| 5. |  | LIMA, M. F.; MOREIRA, W. A. Doenças causadas por bactérias. In: LIMA, M. F.; MOREIRA W. A. (Ed.). Uva de mesa: fitossanidade. Brasília, DF: Embrapa Informação Tecnológica; Petrolina: Embrapa Semi-Árido, 2002. cap. 2, p. 27-34. il. (Frutas do Brasil, 14).| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Semiárido. |
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| 8. | | MOREIRA, W. A.; LIMA, M. F. Nematóides. In: LIMA, M. F.; MOREIRA W. A. (ed.). Uva de mesa: fitossanidade. Brasília, DF: Embrapa Informação Tecnológica: Petrolina: Embrapa Semi-Árido, 2002. cap. 4, p. 45-52. il. (Frutas do Brasil, 14).| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Semiárido. |
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| 9. | | MOREIRA, W. A.; SHARMA, R. D. Nematóides. In: BARBOSA, F. R. (Ed). Goiaba: fitossanidade. Brasília, DF: Embrapa Informação Tecnológica; Petrolina: Embrapa Semi-Árido, 2001. cap. 3, p. 19-28 il. (Frutas do Brasil, 18).| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Cerrados; Embrapa Semiárido. |
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| 10. | | MOREIRA, W. A.; LOPES, D. B.; MOREIRA, F. R. B. Eficiência de fungicidas no controle de fungos que depreciam a qualidade de frutos de manga. Fitopatologia Brasileira, Brasília, DF, v. 27, p.222, ago. 2002. Suplemento. Edição dos Resumos do 35 Congresso Brasileiro de Fitopatologia, Recife, 2002.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| 13. | | MOREIRA, W. A.; BARBOSA, F. R.; MOURA, A. O. Distribuição populacional de fitonematóides em goiabeira no submédio do Vale do São Francisco. Nematologia Brasileira, Marilia, v. 25, n. 1,p. 125, mar. 2001. Edição dos Resumos do 23 Congresso Brasileito de Nematologia, Marilia, mar. 2001.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| 17. | | LIMA, M. F.; MOREIRA, W. A.; COSTA, N. D. Diagnostico de doencas em hortalicas no Submedio Sao Francisco no periodo de 1998 a 1999. Horticultura Brasileira, Brasília, DF, v. 18, p. 437-438, 2000. Suplemento. Edição dos Anais do 40 Congresso Brasileiro de Olericultura; 2 Congresso Ibero-Americano sobre Utilização de Plástico na Agricultura; 1 Simpósio Latino-Americano de Produção de Plantas Medicinais, Aromáticas e Condimentares,...| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| 18. | | MOREIRA, W. A.; CARVALHO, Y. de; ASSUNCAO, M. da S. Efeito de diferentes densidades populacionais de Heterodera glycines sobre a cultura de soja. Fitopatologia Brasileira, Brasília, DF, v. 23, p. 306, ago. 1998. Suplemento. Edição dos Resumos do 31 Congresso Brasileiro de Fitopatologia, Fortaleza, 1998.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| Registros recuperados : 137 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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