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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
29/09/2017 |
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Data da última atualização: |
10/01/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SARAIVA, H. F. R. de A.; BATISTA, R. I. T. P.; ALFRADIQUE, V. A. P.; PINTO, P. H. N.; RIBEIRO, L. S.; OLIVEIRA, C. S.; SOUZA-FABJAN, J. M. G. de; CAMARGO, L. S. de A.; FONSECA, J. F. da; BRANDÃO, F. Z. |
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Afiliação: |
HELENA FABIANA REIS DE ALMEIDA SARAIVA, UNIVERSIDADE FEDERAL FLUMINENSE; RIBRIO IVAN TAVARES PEREIRA BATISTA, UNIVERSIDADE FEDERAL FLUMINENSE; VIVIAN ANGÉLICO PEREIRA ALFRADIQUE, UNIVERSIDADE FEDERAL FLUMINENSE; PEDRO HENRIQUE NICOLAU PINTO, UNIVERSIDADE FEDERAL FLUMINENSE; LILIAN SANTOS RIBEIRO, UNIVERSIDADE FEDERAL FLUMINENSE; CLARA SLADE OLIVEIRA, CNPGL; JOANNA MARIA GONÇALVES DE SOUZA-FABJAN, UNIVERSIDADE FEDERAL FLUMINENSE; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; JEFERSON FERREIRA DA FONSECA, CNPC; FELIPE ZANDONADI BRANDÃO, UNIVERSIDADE FEDERAL FLUMINENSE. |
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Título: |
l-carnitine supplementation during vitrification or warming of in vivo-produced ovine embryos does not affect embryonic survival rates, but alters CrAT and PRDX1 expression. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Theriogenology, v. 105, p. 150-157, Jan. 2018. |
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DOI: |
https://doi.org/10.1016/j.theriogenology.2017.09.022 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: L-carnitine is an antioxidant and beta-oxidation stimulator substance commonly used to improve metabolic performance of oocytes and embryos in in vitro systems. However, few studies have evaluated its beneficial effects in embryos produced in vivo. This study aimed to evaluate the effect of L-carnitine supplementation into vitrification or warming solutions on the post-warming character of day 6?7 in vivo-produced ovine embryos. L-carnitine (3.72 mM) was added to vitrification (Experiment 1) or warming solutions (Experiment 2). In experiments 1 and 2, the embryos were vitrified using straw and cryo-tip protocols, respectively. In vitro culture (IVC) of warmed embryos was performed for 72 h in order to evaluate survival rates, reactive oxygen species (ROS) levels, total cell number (TCN), number of apoptotic cells, apoptotic index evaluation, and gene expression analysis of carnitine palmitoyltransferase I and 2 (CPT1 and CPT2), carnitine O-acetyltransferase (CrAT), and peroxiredoxin-1 (PRDX1). In experiment 1, survival rate, ROS levels after 24 h of IVC, total cell number at 24 h and 72 h, apoptotic cells and apoptotic index at 72 h of IVC were similar in embryos vitrified in medium supplemented with LC or not. Gene expression analysis showed no differences in CPT1 and CPT2mRNA relative abundance in embryos of both experiments compared to fresh embryos (FE); however, CrAT was downregulated (p < 0.05) in C1, and PRDX1 was downregulated (p < 0.05) in both the control (C1) and L-carnitine (LC1) groups, compared to FE. Moreover, CrAT and PRDX1 were upregulated (p < 0.05) in C2, and CrAT was downregulated (p < 0.05) in LC2, in relation to FE. Although the short-term LC supplementation at 3.72 mM did not improve survival, and quality parameters of in vivo-produced ovine embryos, it could affect their quality at a molecular level. In conclusion, further investigations with different concentrations of LC and tools are needed for improvement of the efficiency of these strategies. MenosAbstract: L-carnitine is an antioxidant and beta-oxidation stimulator substance commonly used to improve metabolic performance of oocytes and embryos in in vitro systems. However, few studies have evaluated its beneficial effects in embryos produced in vivo. This study aimed to evaluate the effect of L-carnitine supplementation into vitrification or warming solutions on the post-warming character of day 6?7 in vivo-produced ovine embryos. L-carnitine (3.72 mM) was added to vitrification (Experiment 1) or warming solutions (Experiment 2). In experiments 1 and 2, the embryos were vitrified using straw and cryo-tip protocols, respectively. In vitro culture (IVC) of warmed embryos was performed for 72 h in order to evaluate survival rates, reactive oxygen species (ROS) levels, total cell number (TCN), number of apoptotic cells, apoptotic index evaluation, and gene expression analysis of carnitine palmitoyltransferase I and 2 (CPT1 and CPT2), carnitine O-acetyltransferase (CrAT), and peroxiredoxin-1 (PRDX1). In experiment 1, survival rate, ROS levels after 24 h of IVC, total cell number at 24 h and 72 h, apoptotic cells and apoptotic index at 72 h of IVC were similar in embryos vitrified in medium supplemented with LC or not. Gene expression analysis showed no differences in CPT1 and CPT2mRNA relative abundance in embryos of both experiments compared to fresh embryos (FE); however, CrAT was downregulated (p < 0.05) in C1, and PRDX1 was downregulated (p < 0.05) in both the control... Mostrar Tudo |
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Palavras-Chave: |
Animal embryos; ATP cycle; Drug effects; Mitochondrial genetics; Santa ines; Survival. |
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Thesagro: |
Ovino. |
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Thesaurus Nal: |
Carnitine; Cell culture; Cryopreservation; Ewes; Gene expression; In vitro culture; oocytes; Sheep; Vitrification. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 03336naa a2200433 a 4500
001 2076472
005 2025-01-10
008 2017 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.theriogenology.2017.09.022$2DOI
100 1 $aSARAIVA, H. F. R. de A.
245 $al-carnitine supplementation during vitrification or warming of in vivo-produced ovine embryos does not affect embryonic survival rates, but alters CrAT and PRDX1 expression.$h[electronic resource]
260 $c2017
520 $aAbstract: L-carnitine is an antioxidant and beta-oxidation stimulator substance commonly used to improve metabolic performance of oocytes and embryos in in vitro systems. However, few studies have evaluated its beneficial effects in embryos produced in vivo. This study aimed to evaluate the effect of L-carnitine supplementation into vitrification or warming solutions on the post-warming character of day 6?7 in vivo-produced ovine embryos. L-carnitine (3.72 mM) was added to vitrification (Experiment 1) or warming solutions (Experiment 2). In experiments 1 and 2, the embryos were vitrified using straw and cryo-tip protocols, respectively. In vitro culture (IVC) of warmed embryos was performed for 72 h in order to evaluate survival rates, reactive oxygen species (ROS) levels, total cell number (TCN), number of apoptotic cells, apoptotic index evaluation, and gene expression analysis of carnitine palmitoyltransferase I and 2 (CPT1 and CPT2), carnitine O-acetyltransferase (CrAT), and peroxiredoxin-1 (PRDX1). In experiment 1, survival rate, ROS levels after 24 h of IVC, total cell number at 24 h and 72 h, apoptotic cells and apoptotic index at 72 h of IVC were similar in embryos vitrified in medium supplemented with LC or not. Gene expression analysis showed no differences in CPT1 and CPT2mRNA relative abundance in embryos of both experiments compared to fresh embryos (FE); however, CrAT was downregulated (p < 0.05) in C1, and PRDX1 was downregulated (p < 0.05) in both the control (C1) and L-carnitine (LC1) groups, compared to FE. Moreover, CrAT and PRDX1 were upregulated (p < 0.05) in C2, and CrAT was downregulated (p < 0.05) in LC2, in relation to FE. Although the short-term LC supplementation at 3.72 mM did not improve survival, and quality parameters of in vivo-produced ovine embryos, it could affect their quality at a molecular level. In conclusion, further investigations with different concentrations of LC and tools are needed for improvement of the efficiency of these strategies.
650 $aCarnitine
650 $aCell culture
650 $aCryopreservation
650 $aEwes
650 $aGene expression
650 $aIn vitro culture
650 $aoocytes
650 $aSheep
650 $aVitrification
650 $aOvino
653 $aAnimal embryos
653 $aATP cycle
653 $aDrug effects
653 $aMitochondrial genetics
653 $aSanta ines
653 $aSurvival
700 1 $aBATISTA, R. I. T. P.
700 1 $aALFRADIQUE, V. A. P.
700 1 $aPINTO, P. H. N.
700 1 $aRIBEIRO, L. S.
700 1 $aOLIVEIRA, C. S.
700 1 $aSOUZA-FABJAN, J. M. G. de
700 1 $aCAMARGO, L. S. de A.
700 1 $aFONSECA, J. F. da
700 1 $aBRANDÃO, F. Z.
773 $tTheriogenology$gv. 105, p. 150-157, Jan. 2018.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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| Registros recuperados : 31 | |
| 6. |  | FREIRE, G. S.; SANTOS, M. T. P. dos; SÁ, T. D. de A.; DAVIDSON, E. A. Características qualitativas da chuva sob dossel em vegetações secundárias sucedendo diferentes usos da terra no nordeste do Pará. In: CONGRESSO DE ESTUDANTES E BOLSISTAS DO EXPERIMENTO LBA, 1., 2002, Belém, PA. Resumos. Belém, PA: LBA, 2002. p. 30.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 9. | | SANTOS, M. T.; FERRAZ, L. F. C.; REIS, F. C.; FALCÃO, P. R. K.; NESHICH, G.; OTTOBONI, L. M. M. Análise estrutural e funcional da proteína CMP quinase de Acidithiobacillus ferrooxidans. In: CONGRESSO BRASILEIRO DE GENÉTICA, 51., 2005, Águas de Lindóia. Resumos... Águas de Lindóias: SBG, 2005. p. 1075. Na publicação: Falcão, PK.| Biblioteca(s): Embrapa Agricultura Digital. |
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| 10. | | PANTOJA, K. de F. R.; CARVALHO, C. J. R. de; VIEIRA, I. C. G.; SANTOS, M. T. P. dos. Avaliação dos estoques de N e P na camada de litter produzida em cronosequência de florestas na região Bragantina, leste do Estado do Pará. In: CONGRESSO DE ESTUDANTES E BOLSISTAS DO EXPERIMENTO LBA, 1., 2002, Belém, PA. Resumos. Belém, PA: LBA, 2002. p. 28.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 11. | | FREIRE, G. S.; CARVALHO, C. J. R. de; SANTOS, M. T. P. dos; PANTOJA, K. de F. R. Avaliação dos teores nitrogênio e polifenois no litter de floresta primária e cronoseqüência de florestas secundárias da Amazônia Oriental. In: CONGRESSO DE ESTUDANTES E BOLSISTAS DO EXPERIMENTO LBA, 1., 2002, Belém, PA. Resumos. Belém, PA: LBA, 2002. p. 40.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 12. | | YAGUINUMA, C. A.; MAGALHAES JUNIOR, W. C. P. de; SANTOS, M. T. P.; CAMARGO, H. A.; REFORMAT, M. Combining fuzzy ontology reasoning and mamdani fuzzy inference system with HyFOM reasoner. In: HAMMOUDI, S., CORDEIRO, J., MACIASZEK, L., FILIPE, J. (ed.). Enterprise information systems. Cham: Springer, 2014. 190 (Lecture Notes in Business Information Processing, v. 190.)| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 13. | | YAGUINUMA, C. A.; MAGALHAES JUNIOR, W. C. P. de; SANTOS, M. T. P.; CAMARGO, H. A.; REFORMAT, M. HyFOM reasoner: hybrid integration of fuzzy ontology and Mamdani reasoning. In: INTERNATIONAL CONFERENCE ON ENTERPRISE INFORMATION SYSTEMS, 15.; INTERNATIONAL CONFERENCE ON EVALUATION OF NOVEL APPROACHES TO SOFTWARE ENGINEERING, 8., 2013, Angers Loire Valley. Proceedings... [S.l.]: Scitepress, 2013. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 15. | | CRUZ, J. L.; COELHO, E. F.; PELACANI, C. R.; COELHO FILHO, M. A.; DIAS, A. T.; SANTOS, M. T. dos. Crescimento e partição de matéria seca e de carbono no mamoeiro em resposta à nutrição nitrogenada. Bragantia, Campinas, v. 63, n. 3, p. 351-361, set./dez. 2004.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 16. | | ARAGÃO, D. V.; CARVALHO, C. J. R. de; KATO, O. R.; ARAÚJO, C. M. de; SANTOS, M. T. P. dos; MOURÃO JUNIOR, M. Avaliação de indicadores de qualidade do solo sob alternativas de recuperação do solo no Nordeste paraense. Acta amazonica, Manaus, v. 42, n. 1, p. 11-18, mar. 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 17. | | DUTRA, F. C.; SERRÃO, B. de O.; SANTOS, M. T. P. dos; SOUZA, C. M. A.; TRINDADE, I. A.; CARVALHO, C. J. R. de. Atividade de fosfatase ácida, uréase e micorrizas em uma área de pastagem abandonada (Paragominas - PA), após adubação com nitrogênio e fósforo. In: CONGRESSO DE ESTUDANTES E BOLSISTAS DO EXPERIMENTO LBA, 2., 2005, Manaus. Resumos... [S.l.]: LBA, [2005?]. p. 41.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 18. | | OLIVEIRA, V. C. de; ISHIDA, F. V.; DAVIDSON, E.; BELK, E.; SANTOS, M. T. P. dos; SÁ, T. D. de A.; MÖLLER, M. R. Emissões de gases traços em capoeiras enriquecidas no Nordeste paraense. In: LBA SCIENTIFIC CONFERENCE, 1., 2000, Belém, PA. Abstracts... Belém, PA: LBA, 2000. p. 44.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 19. | | SERRÃO, B. de O.; DUTRA, F. C.; SANTOS, M. T. P. dos; SOUZA, C. M. de A.; TRINDADE, I. A.; CARVALHO, C. J. R. de; DAVIDSON, E. A. Carbono e nitrogênio na biomassa microbiana do solo de uma área de pastagem abandonada, três anos após fertilização com N e P. In: CONGRESSO DE ESTUDANTES E BOLSISTAS DO EXPERIMENTO LBA, 2., 2005, Manaus. Resumos... [S.l.]: LBA, [2005?]. p. 45.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 20. | | CANO, M. D.; SANTOS, M. T. P.; AVILA, A. M. H. de; ROMANI, L. A. S.; TRAINA, A. J. M.; RIBEIRO, M. X. SART: a new association rule method for mining sequential patterns in time series of climate data. In: INTERNATIONAL CONFERENCE, 12., 2012, Salvador de Bahia. Computational science and its applications: proceedings. Berlin: Springer, 2012. p. 743-757. (Lecture notes in computer science, 7335). Part III. ICCSA 2012. Editores: Beniamino Murgante, Osvaldo Gervasi, Sanjay Misra, Nadia, Nejah, Ana Maria A. C. Rocha, David Taniar, Bernardy I, Apduhan.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agricultura Digital. |
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| Registros recuperados : 31 | |
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