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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
26/10/2016 |
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Data da última atualização: |
05/04/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GONÇALVES, B.-H. R. F.; SILVA, G. de J.; PONTES, S. F. O.; FONTAN, R. da C. I.; EGITO, A. S. do; FERRÃO, S. P. B. |
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Afiliação: |
Ben-Hur Ramos Ferreira Gonçalves, Universidade Estadual do Sudoeste da Bahia (UESB) - Itapetinga, BA, Brasil; Grazielly de Jesus Silva, UESB - Itapetinga, BA, Brasil; Silvania Farias Oliveira Pontes, UESB - Itapetinga, BA, Brasil; Rafael da Costa Ilheu Fontan, UESB - Itapetinga, BA, Brasil; ANTONIO SILVIO DO EGITO, CNPC; Sibelli Passini Barbosa Ferrão, UESB - Itapetinga, BA, Brasil. |
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Título: |
Evaluation of the peptide profile with a view to authenticating buffalo mozzarella cheese. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
International Journal of Food Science and Technology, v. 51, n. 7, p. 1586-1593, Jul. 2016. |
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DOI: |
10.1111/ijfs.13128 |
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Idioma: |
Inglês |
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Notas: |
[ANTONIO SILVIO DO EGITO VASCONCELOS]. |
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Conteúdo: |
Abstract: Electrophoretic and chromatographic techniques were used to determine water-soluble peptide profiles aiming to identify the adulteration of buffalo milk mozzarella cheese by the addition of cow's milk. Thus, cheeses were produced with contents of cow's milk varying from 0% to 100%, and the peptides extracted after production and after 20 days of refrigeration. Polyacrylamide gel electrophoresis under denaturing conditions in the presence of sodium dodecyl sulphate (SDS-PAGE) identified a potential peptide marker of exclusively bovine origin with a size of about 21 kDa for the addition of cow's milk above 30%. Reverse-phase high-performance liquid chromatography (RP-HPLC) indicated the existence of two potential peptides present in higher concentrations in buffalo milk and one exclusive for cow's milk, the latter making it possible to estimate the addition of cow's milk to buffalo milk. Six commercial brands of buffalo mozzarella cheese were evaluated, and indications of adulteration found in four of them. |
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Palavras-Chave: |
Adulteration; Cheese; Cromatografia líquida de alta eficiência; Feed adulteration; High-performance liquid chromatography; Leite de búfala; Milk products; Milk protein; Mozzarella; Proteína do leite. |
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Thesagro: |
Adulteração; Cromatografia; Produto derivado do leite; Queijo; Tecnologia de alimento. |
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Thesaurus Nal: |
Buffalo milk; Polyacrylamide gel electrophoresis. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02305naa a2200409 a 4500
001 2055390
005 2019-04-05
008 2016 bl uuuu u00u1 u #d
024 7 $a10.1111/ijfs.13128$2DOI
100 1 $aGONÇALVES, B.-H. R. F.
245 $aEvaluation of the peptide profile with a view to authenticating buffalo mozzarella cheese.
260 $c2016
500 $a[ANTONIO SILVIO DO EGITO VASCONCELOS].
520 $aAbstract: Electrophoretic and chromatographic techniques were used to determine water-soluble peptide profiles aiming to identify the adulteration of buffalo milk mozzarella cheese by the addition of cow's milk. Thus, cheeses were produced with contents of cow's milk varying from 0% to 100%, and the peptides extracted after production and after 20 days of refrigeration. Polyacrylamide gel electrophoresis under denaturing conditions in the presence of sodium dodecyl sulphate (SDS-PAGE) identified a potential peptide marker of exclusively bovine origin with a size of about 21 kDa for the addition of cow's milk above 30%. Reverse-phase high-performance liquid chromatography (RP-HPLC) indicated the existence of two potential peptides present in higher concentrations in buffalo milk and one exclusive for cow's milk, the latter making it possible to estimate the addition of cow's milk to buffalo milk. Six commercial brands of buffalo mozzarella cheese were evaluated, and indications of adulteration found in four of them.
650 $aBuffalo milk
650 $aPolyacrylamide gel electrophoresis
650 $aAdulteração
650 $aCromatografia
650 $aProduto derivado do leite
650 $aQueijo
650 $aTecnologia de alimento
653 $aAdulteration
653 $aCheese
653 $aCromatografia líquida de alta eficiência
653 $aFeed adulteration
653 $aHigh-performance liquid chromatography
653 $aLeite de búfala
653 $aMilk products
653 $aMilk protein
653 $aMozzarella
653 $aProteína do leite
700 1 $aSILVA, G. de J.
700 1 $aPONTES, S. F. O.
700 1 $aFONTAN, R. da C. I.
700 1 $aEGITO, A. S. do
700 1 $aFERRÃO, S. P. B.
773 $tInternational Journal of Food Science and Technology$gv. 51, n. 7, p. 1586-1593, Jul. 2016.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Semiárido. Para informações adicionais entre em contato com cpatsa.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
22/06/2020 |
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Data da última atualização: |
01/10/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
SENA, P. T. S.; NASCIMENTO, T. R. do; LINO, J. de O. S.; OLIVEIRA, G. S.; FERREIRA NETO, R. A.; FREITAS, A. D. S. de; FERNANDES JUNIOR, P. I.; MARTINS, L. M. V. |
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Afiliação: |
Pâmella Thalita Souza Sena; Tailane Ribeiro do Nascimento; Jaynne de Oliveira Siqueira Lino; Gilmar Silva Oliveira; Reginaldo Alves Ferreira Neto; Ana Dolores Santiago de Freitas; PAULO IVAN FERNANDES JUNIOR, CPATSA; Lindete Míria Vieira Martins. |
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Título: |
Molecular, physiological, and symbiotic characterization of cowpea rhizobia from soils under different agricultural systems in the Semiarid region of Brazil. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Journal of Soil Science and Plant Nutrition, v, 20, n. 3, p. 1178-1192, 2020. |
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DOI: |
https://doi.org/10.1007/s42729-020-00203-3 |
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Idioma: |
Inglês |
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Conteúdo: |
The objectives of this study were to characterize the genetic diversity and evaluate the ability to tolerate stress as well as to assess the symbiotic efficiency of bacteria from cowpea nodules in agricultural soils with different uses in the semiarid region of Bahia state (Brazil). Soil samples were collected from six crop lands and one from the pristine Caatinga biome. After a trap-host experiment, the bacteria were isolated and culturally characterized. Isolates with typical characteristics of Bradyrhizobium were subjected to the nodC symbiotic gene amplification and those positive were evaluated by 16S-23S IGS-RFLP. Twenty-seven isolates belonging to different genetic clusters were selected for 16S-23S IGS sequencing. In additions, the selected bacteria were characterized biochemically and symbiotically. Among 420 characterized isolates, approximately 60% (251 isolates) displayed typical Bradyrhizobium cultural features. A total of 161, out of 251 isolates, showed positive amplification of the nodC gene fragment. The IGS-RFLP profiles analysis generated 33 groups and 27 were selected for further analysis. The fertility of the soils influenced the distribution of the isolates in the IGS-RFLP clusters. The bacteria were assigned to two genera, Bradyrhizobium and Microvirga, with 26 and 1 representative bacteria, respectively. Some isolates were able to tolerate NaCl as well as acidic and alkaline pH. In addition, isolates showed the abilities to produce biofilm under stress and to produce indole compounds, as well as efficient nodulation and nitrogen fixation. The isolates displayed great genetic, biochemical, and symbiotic variability with promising biotechnological potential. MenosThe objectives of this study were to characterize the genetic diversity and evaluate the ability to tolerate stress as well as to assess the symbiotic efficiency of bacteria from cowpea nodules in agricultural soils with different uses in the semiarid region of Bahia state (Brazil). Soil samples were collected from six crop lands and one from the pristine Caatinga biome. After a trap-host experiment, the bacteria were isolated and culturally characterized. Isolates with typical characteristics of Bradyrhizobium were subjected to the nodC symbiotic gene amplification and those positive were evaluated by 16S-23S IGS-RFLP. Twenty-seven isolates belonging to different genetic clusters were selected for 16S-23S IGS sequencing. In additions, the selected bacteria were characterized biochemically and symbiotically. Among 420 characterized isolates, approximately 60% (251 isolates) displayed typical Bradyrhizobium cultural features. A total of 161, out of 251 isolates, showed positive amplification of the nodC gene fragment. The IGS-RFLP profiles analysis generated 33 groups and 27 were selected for further analysis. The fertility of the soils influenced the distribution of the isolates in the IGS-RFLP clusters. The bacteria were assigned to two genera, Bradyrhizobium and Microvirga, with 26 and 1 representative bacteria, respectively. Some isolates were able to tolerate NaCl as well as acidic and alkaline pH. In addition, isolates showed the abilities to produce biofilm under stres... Mostrar Tudo |
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Palavras-Chave: |
Diversidade genética; Fixação biológica de nitrogênio; Microvirga; Semiárido. |
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Thesagro: |
Feijão; Inoculante. |
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Thesaurus NAL: |
Bradyrhizobium. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 02731naa a2200301 a 4500
001 2123416
005 2021-10-01
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s42729-020-00203-3$2DOI
100 1 $aSENA, P. T. S.
245 $aMolecular, physiological, and symbiotic characterization of cowpea rhizobia from soils under different agricultural systems in the Semiarid region of Brazil.$h[electronic resource]
260 $c2020
520 $aThe objectives of this study were to characterize the genetic diversity and evaluate the ability to tolerate stress as well as to assess the symbiotic efficiency of bacteria from cowpea nodules in agricultural soils with different uses in the semiarid region of Bahia state (Brazil). Soil samples were collected from six crop lands and one from the pristine Caatinga biome. After a trap-host experiment, the bacteria were isolated and culturally characterized. Isolates with typical characteristics of Bradyrhizobium were subjected to the nodC symbiotic gene amplification and those positive were evaluated by 16S-23S IGS-RFLP. Twenty-seven isolates belonging to different genetic clusters were selected for 16S-23S IGS sequencing. In additions, the selected bacteria were characterized biochemically and symbiotically. Among 420 characterized isolates, approximately 60% (251 isolates) displayed typical Bradyrhizobium cultural features. A total of 161, out of 251 isolates, showed positive amplification of the nodC gene fragment. The IGS-RFLP profiles analysis generated 33 groups and 27 were selected for further analysis. The fertility of the soils influenced the distribution of the isolates in the IGS-RFLP clusters. The bacteria were assigned to two genera, Bradyrhizobium and Microvirga, with 26 and 1 representative bacteria, respectively. Some isolates were able to tolerate NaCl as well as acidic and alkaline pH. In addition, isolates showed the abilities to produce biofilm under stress and to produce indole compounds, as well as efficient nodulation and nitrogen fixation. The isolates displayed great genetic, biochemical, and symbiotic variability with promising biotechnological potential.
650 $aBradyrhizobium
650 $aFeijão
650 $aInoculante
653 $aDiversidade genética
653 $aFixação biológica de nitrogênio
653 $aMicrovirga
653 $aSemiárido
700 1 $aNASCIMENTO, T. R. do
700 1 $aLINO, J. de O. S.
700 1 $aOLIVEIRA, G. S.
700 1 $aFERREIRA NETO, R. A.
700 1 $aFREITAS, A. D. S. de
700 1 $aFERNANDES JUNIOR, P. I.
700 1 $aMARTINS, L. M. V.
773 $tJournal of Soil Science and Plant Nutrition, v, 20$gn. 3, p. 1178-1192, 2020.
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Embrapa Semiárido (CPATSA) |
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