| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Cerrados. Para informações adicionais entre em contato com biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Cerrados. |
|
Data corrente: |
11/12/2020 |
|
Data da última atualização: |
11/12/2020 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
ARANTES, L. G.; TONELLI, G. S. S. S.; MARTINS, C. F.; BÁO, S. N. |
|
Afiliação: |
CARLOS FREDERICO MARTINS, CPAC. |
|
Título: |
Cellular Characterization and Effects Of Cryoprotectant Solutions on the Viability Of Fibroblasts from Three Brazilian Wild Cats. |
|
Ano de publicação: |
2020 |
|
Fonte/Imprenta: |
Biopreservation and Biobanking, 2020. |
|
Idioma: |
Português |
|
Conteúdo: |
Preserving genetic material in cryogenic conditions presents a viable alternative for the protection of species' gene variability. However, there is an enormous need to establish and test cryopreservation protocols that are suitable for each diverse cell type to guarantee technical success in the long run. Considering this, fibroblasts from jaguar (Panthera onca), oncilla (Leopardus tigrinus), and pampas cat (Leopardus colocolo) were subjected to cell characterization and then cryopreservation in different cryoprotectant solutions (2.5%, 10% dimethyl sulfoxide [DMSO] or CryoSOfree?). Further testing was conducted to determine each solution's performance in preserving cell viability. In culture, a growth curve to assess cellular growth potential showed that exponential proliferation lasts for about the first 50 hours of in vitro culturing, declining in pace afterward. L. colocolo and L. tigrinus presented no difference in cell viability while using 2.5% DMSO protocols. P. onca cells did not present difference on viability for both concentrations of DMSO. Protocols using CryoSOfree resulted in a decreased viability of P. onca fibroblasts. Morphological differences between fibroblasts among the species were noted under bright field microscopy and scanning electron microscopy. L. colocolo and P. onca cells are fusiform, and L. tigrinus are spherical. All cells presented cytoplasmic projections. Transmission electron microscopy revealed vacuoles and secretion granules, indicating intense cell activity after thawing. Differences found in the efficiency of cryopreservation protocols according to the type of cryoprotectant indicate that species react differently to freezing and thawing processes. This research evaluates key aspects of in vitro protocols for cryopreservation of wild animals, which need to be optimized to guarantee successful cell culturing. More suitable protocols lead to increased efficiency in establishing fibroblast cryobanks and also facilitating the use of wild cats' cells in cloning techniques, contributing directly to preserving wild fauna. MenosPreserving genetic material in cryogenic conditions presents a viable alternative for the protection of species' gene variability. However, there is an enormous need to establish and test cryopreservation protocols that are suitable for each diverse cell type to guarantee technical success in the long run. Considering this, fibroblasts from jaguar (Panthera onca), oncilla (Leopardus tigrinus), and pampas cat (Leopardus colocolo) were subjected to cell characterization and then cryopreservation in different cryoprotectant solutions (2.5%, 10% dimethyl sulfoxide [DMSO] or CryoSOfree?). Further testing was conducted to determine each solution's performance in preserving cell viability. In culture, a growth curve to assess cellular growth potential showed that exponential proliferation lasts for about the first 50 hours of in vitro culturing, declining in pace afterward. L. colocolo and L. tigrinus presented no difference in cell viability while using 2.5% DMSO protocols. P. onca cells did not present difference on viability for both concentrations of DMSO. Protocols using CryoSOfree resulted in a decreased viability of P. onca fibroblasts. Morphological differences between fibroblasts among the species were noted under bright field microscopy and scanning electron microscopy. L. colocolo and P. onca cells are fusiform, and L. tigrinus are spherical. All cells presented cytoplasmic projections. Transmission electron microscopy revealed vacuoles and secretion granules, indicating... Mostrar Tudo |
|
Palavras-Chave: |
Material genético; Protocolo. |
|
Thesagro: |
Criopreservação. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 02696naa a2200193 a 4500
001 2128006
005 2020-12-11
008 2020 bl uuuu u00u1 u #d
100 1 $aARANTES, L. G.
245 $aCellular Characterization and Effects Of Cryoprotectant Solutions on the Viability Of Fibroblasts from Three Brazilian Wild Cats.$h[electronic resource]
260 $c2020
520 $aPreserving genetic material in cryogenic conditions presents a viable alternative for the protection of species' gene variability. However, there is an enormous need to establish and test cryopreservation protocols that are suitable for each diverse cell type to guarantee technical success in the long run. Considering this, fibroblasts from jaguar (Panthera onca), oncilla (Leopardus tigrinus), and pampas cat (Leopardus colocolo) were subjected to cell characterization and then cryopreservation in different cryoprotectant solutions (2.5%, 10% dimethyl sulfoxide [DMSO] or CryoSOfree?). Further testing was conducted to determine each solution's performance in preserving cell viability. In culture, a growth curve to assess cellular growth potential showed that exponential proliferation lasts for about the first 50 hours of in vitro culturing, declining in pace afterward. L. colocolo and L. tigrinus presented no difference in cell viability while using 2.5% DMSO protocols. P. onca cells did not present difference on viability for both concentrations of DMSO. Protocols using CryoSOfree resulted in a decreased viability of P. onca fibroblasts. Morphological differences between fibroblasts among the species were noted under bright field microscopy and scanning electron microscopy. L. colocolo and P. onca cells are fusiform, and L. tigrinus are spherical. All cells presented cytoplasmic projections. Transmission electron microscopy revealed vacuoles and secretion granules, indicating intense cell activity after thawing. Differences found in the efficiency of cryopreservation protocols according to the type of cryoprotectant indicate that species react differently to freezing and thawing processes. This research evaluates key aspects of in vitro protocols for cryopreservation of wild animals, which need to be optimized to guarantee successful cell culturing. More suitable protocols lead to increased efficiency in establishing fibroblast cryobanks and also facilitating the use of wild cats' cells in cloning techniques, contributing directly to preserving wild fauna.
650 $aCriopreservação
653 $aMaterial genético
653 $aProtocolo
700 1 $aTONELLI, G. S. S. S.
700 1 $aMARTINS, C. F.
700 1 $aBÁO, S. N.
773 $tBiopreservation and Biobanking, 2020.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Cerrados (CPAC) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 75 | |
| 2. |  | SANTOS, A. M.; MITJA, D.; CARVALHO, D. C. Extrato arbóreo nas pastagens: o caso da agricultura familiar (comunidade de Benfica, sul do Pará). In: CONGRESSO BRASILEIRO DA CIÊNCIA DAS PLANTAS DANINHAS, 25., 2006, Brasília, DF. Convivendo com as plantas daninhas: resumos. Brasília, DF: Sociedade Brasileira da Ciência das Plantas Daninhas: UnB: Embrapa Cerrados, 2006. p. 57| Biblioteca(s): Embrapa Cerrados. |
|   |
| 5. | | SANTOS, A. R. dos; BOAVENTURA, G. R.; PINELLI, M. P.; DIAS, R. P.; RAMAGEM, R. D. Caracterizacao do teor disponivel de metais na compostagem de lixo domiciliar urbano no processo tradicional e na coleta seletiva. In: CONGRESSO BRASILEIRO DE CIENCIA DO SOLO, 25., 1995, Vicosa, MG. O solo nos grandes dominios morfoclimaticos do Brasil e o desenvolvimento sustentado: resumos expandidos. Vicosa: SBCS, 1995. v.1. p.211-213.| Biblioteca(s): Embrapa Cerrados. |
| |
| 10. | | SANTOS, A. R. dos; RESENDE, M.; KER, J. C.; FONTES, M. P. F. Proposta de metodologia para semiquantificação das espécies minerais de solos intemperizados. In: CONGRESSO LATINO-AMERICANO DE CIENCIA DO SOLO, 13.; REUNIAO BRASILEIRA DE BIOLOGIA DO SOLO, 1.; SIMPOSIO BRASILEIRO SOBRE MICROBIOLOGIA DO SOLO, 4.; REUNIAO BRASILEIRA SOBRE MICORRIZAS, 6.; REUNIAO BRASILEIRA DE MANEJO E CONSERVACAO DO SOLO E DA AGUA, 11., 1996, Aguas de ...| Biblioteca(s): Embrapa Cerrados. |
|  |
| 13. | | ZANATTA, J. A.; SANTOS, A. M.; PULROLNIK, K.; MORESCO, E. R.; MOREIRA, J. M. M. A. P. Práticas sustentáveis para o eucalipto. In: TORRES, D. A. P.; BALSADI, O. V. (ed.). Embrapa e políticas públicas: contribuições para a taxonomia sustentável brasileira. Brasília, DF: Embrapa, 2025. cap. 8, p. 96-115.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Cerrados; Embrapa Florestas. |
| |
| 15. | | LOPES, A. A. C.; REIS JÚNIOR, F. B.; MENDES, I. C.; SANTOS, A. B.; XAVIER, G. R.; BAPTISTA, M. J. Atividade da enzima beta-glicosidase em solo sob solarização e biofumigação. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 24., 2007, Brasília. Anais... Brasília: Sociedade Brasileira de Microbiologia, 2007. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Cerrados. |
| |
| 17. | | SANTOS, A. R. dos; FONTES, M. P. F.; ALVAREZ VENEGAS, V. H.; RESENDE, M.; KER, J. C.; COSTA, L. M. Caracterização mineralógica, potencial de reserva e sustentabilidade agrícola de sítios florestais de Eucalyptus grandis do Vale do Rio Doce - MG. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 22., 1996, Manaus. Resumos expandidos. Manaus: SBCS/UA/EMBRAPA-CPAA/INPA, 1996. p. 76-77.| Biblioteca(s): Embrapa Cerrados. |
| |
| Registros recuperados : 75 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
