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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
11/07/2013 |
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Data da última atualização: |
02/08/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OLIVEIRA, L. R.; RODRIGUES, E. P.; MARCELINO-GUIMARÃES, F. C.; OLIVEIRA, A. L. M.; HUNGRIA, M. |
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Afiliação: |
LUCIANA RUANO OLIVEIRA, UEL; ELISETE PAINS RODRIGUES, UEL; FRANCISMAR CORREA MARCELINO-GUIMARÃES, CNPSO; ANDRÉ LUIZ MARTINEZ OLIVEIRA, UEL; MARIANGELA HUNGRIA, CNPSO. |
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Título: |
Fast induction of biosynthetic polysaccharide genes lpxA, lpxE, and rkpI of Rhizobium sp. strain PRF 81 by common bean seed exudates is indicative of a key role in symbiosis. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Functional & Integrative Genomics, v. 13, n. 2, p. 275-283, Jun. 2013. |
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ISSN: |
1438-7948 |
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DOI: |
10.1007/s10142-013-0322-7 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Rhizobial surface polysaccharides (SPS) are, together with nodulation (Nod) factors, recognized as key molecules for establishment of rhizobia?legume symbiosis. In Rhizobium tropici, an important nitrogen-fixing symbiont of common bean (Phaseolus vulgaris L.), molecular structures and symbiotic roles of the SPS are poorly understood. In this study, Rhizobium sp. strain PRF 81 genes, belonging to the R. tropici group, were investigated: lpxA and lpxE, involved in biosynthesis and modification of the lipid-A anchor of lipopolysaccharide (LPS), and rkpI, involved in synthesis of a lipid carrier required for production of capsular polysaccharides (KPS). Reverse transcription quantitative PCR (RT-qPCR) analysis revealed, for the first time, that inducers released from common bean seeds strongly stimulated expression of all three SPS genes. When PRF 81 cells were grown for 48 h in the presence of seed exudates, twofold increases (p < 0.05) in the transcription levels of lpxE, lpxA, and rkpI genes were observed. However, higher increases (p < 0.05) in transcription rates, about 50-fold for lpxE and about 30-fold for lpxA and rkpI, were observed after only 5 min of incubation with common bean seed exudates. Evolutionary analyses revealed that lpxA and lpxE of PRF81 and of the type strain of R. tropici CIAT899Tclustered with orthologous Rhizobium radiobacter and were more related to R. etli and Rhizobium leguminosarum, while rkpI was closer to the Sinorhizobium sp. group. Upregulation of lpxE, lpxA, and rkpI genes suggests that seed exudates can modulate production of SPS of Rhizobium sp. PRF81, leading to cell wall changes necessary for symbiosis establishment. MenosAbstract: Rhizobial surface polysaccharides (SPS) are, together with nodulation (Nod) factors, recognized as key molecules for establishment of rhizobia?legume symbiosis. In Rhizobium tropici, an important nitrogen-fixing symbiont of common bean (Phaseolus vulgaris L.), molecular structures and symbiotic roles of the SPS are poorly understood. In this study, Rhizobium sp. strain PRF 81 genes, belonging to the R. tropici group, were investigated: lpxA and lpxE, involved in biosynthesis and modification of the lipid-A anchor of lipopolysaccharide (LPS), and rkpI, involved in synthesis of a lipid carrier required for production of capsular polysaccharides (KPS). Reverse transcription quantitative PCR (RT-qPCR) analysis revealed, for the first time, that inducers released from common bean seeds strongly stimulated expression of all three SPS genes. When PRF 81 cells were grown for 48 h in the presence of seed exudates, twofold increases (p < 0.05) in the transcription levels of lpxE, lpxA, and rkpI genes were observed. However, higher increases (p < 0.05) in transcription rates, about 50-fold for lpxE and about 30-fold for lpxA and rkpI, were observed after only 5 min of incubation with common bean seed exudates. Evolutionary analyses revealed that lpxA and lpxE of PRF81 and of the type strain of R. tropici CIAT899Tclustered with orthologous Rhizobium radiobacter and were more related to R. etli and Rhizobium leguminosarum, while rkpI was closer to the Si... Mostrar Tudo |
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Thesagro: |
Feijão. |
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Categoria do assunto: |
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Marc: |
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022 $a1438-7948
024 7 $a10.1007/s10142-013-0322-7$2DOI
100 1 $aOLIVEIRA, L. R.
245 $aFast induction of biosynthetic polysaccharide genes lpxA, lpxE, and rkpI of Rhizobium sp. strain PRF 81 by common bean seed exudates is indicative of a key role in symbiosis.$h[electronic resource]
260 $c2013
520 $aAbstract: Rhizobial surface polysaccharides (SPS) are, together with nodulation (Nod) factors, recognized as key molecules for establishment of rhizobia?legume symbiosis. In Rhizobium tropici, an important nitrogen-fixing symbiont of common bean (Phaseolus vulgaris L.), molecular structures and symbiotic roles of the SPS are poorly understood. In this study, Rhizobium sp. strain PRF 81 genes, belonging to the R. tropici group, were investigated: lpxA and lpxE, involved in biosynthesis and modification of the lipid-A anchor of lipopolysaccharide (LPS), and rkpI, involved in synthesis of a lipid carrier required for production of capsular polysaccharides (KPS). Reverse transcription quantitative PCR (RT-qPCR) analysis revealed, for the first time, that inducers released from common bean seeds strongly stimulated expression of all three SPS genes. When PRF 81 cells were grown for 48 h in the presence of seed exudates, twofold increases (p < 0.05) in the transcription levels of lpxE, lpxA, and rkpI genes were observed. However, higher increases (p < 0.05) in transcription rates, about 50-fold for lpxE and about 30-fold for lpxA and rkpI, were observed after only 5 min of incubation with common bean seed exudates. Evolutionary analyses revealed that lpxA and lpxE of PRF81 and of the type strain of R. tropici CIAT899Tclustered with orthologous Rhizobium radiobacter and were more related to R. etli and Rhizobium leguminosarum, while rkpI was closer to the Sinorhizobium sp. group. Upregulation of lpxE, lpxA, and rkpI genes suggests that seed exudates can modulate production of SPS of Rhizobium sp. PRF81, leading to cell wall changes necessary for symbiosis establishment.
650 $aFeijão
700 1 $aRODRIGUES, E. P.
700 1 $aMARCELINO-GUIMARÃES, F. C.
700 1 $aOLIVEIRA, A. L. M.
700 1 $aHUNGRIA, M.
773 $tFunctional & Integrative Genomics$gv. 13, n. 2, p. 275-283, Jun. 2013.
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Registro original: |
Embrapa Soja (CNPSO) |
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| Registros recuperados : 11 | |
| 2. |  | AVILA, A. de; RUSCHEL, A.; CARVALHO, J. de; SILVA, J.; MAZZEI, L.; CASTRO, T.; BAUHUS, J. Post-logging stand dynamics of a tropical rain forest in the Brazilian Amazon. The International Forestry Review, v. 16, n. 5, p. 138, 2014. Edição dos abstracts do 24º IUFRO World Congress, 2014, Salt Lake City. Sustaining forests, sustaining people: the role of research.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 3. | | COSTA, C. L.; COSTA, A. F.; MARINHO, V. L. A.; KITAJIMA, E. W.; LIN, M. T.; AVILA, A. C. de; BRIOSO, P. S. T.; ALBUQUERQUE, F. C. de. Potyvirus isolados de feijão de porco (Canavalia spp.). Fitopatologia Brasileira, Brasilia, v. 9, n. 2, p. 400, jun. 1984. Resumo.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental; Embrapa Hortaliças. |
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| 4. | | AVILA, A. L. de; RUSCHEL, A. R.; SILVA, J. N. M.; FREITAS, L. J. M. de; CARVALHO, J. O. P. de; BAUHUS, J. Disturbance intensity determines recovery of multiple functions after a first logging cycle in the Brazilian Amazon. Pesquisa Florestal Brasileira, v. 39, e201902043, p. 153, 2019. Special issue. Abstracts of the XXV IUFRO World Congress, 2019.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 5. | | AVILA, A. L. de; RUSCHEL, A. R.; CARVALHO, J. O. P. de; SILVA, J. N.; MAZZEI, L.; DORMANN, C. F.; BAUHUS, J. Forest recovery over 30 years following management interventions of different intensities in the brazilian Amazon. In: ANNUAL CONFERENCE OF THE SOCIETY FOR TROPICAL ECOLOGY, 2015, Zürich. Resilience of tropical ecosystems - future challenges and opportunities. Zurich: GTO, 2015. p. 43.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 6. | | SANDE, M. T. van der; PEÑA-CLAROS, M.; ARETS, E. J. M. M.; MAZZEI, L.; ASCARRUNZ, N.; TOLEDO, M.; LICONA, J. C.; ALARCÓN, A.; POORTER, L.; AVILA, A. L. de; ROOPSIND, A.; RUSCHEL, A.; FINEGAN, B.; CÁCERES-SIANI, Y. Are Neotropical forests changing in species and trait composition? In: ANNUAL MEETING OF THE ASSOCIATION FOR TROPICAL BIOLOGY AND CONSERVATION, 52., 2015, Honolulu. Resilience of Island Systems in the Context of Climate Change: Challenges for Biological and Cultural diversity and Conservation: abstracts. [S.l.]: ATBC, 2015. p. 34.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 7. | | AVILA, A. L. de; SANDE, M. T. van der; DORMANN, C. F.; FREITAS, L. J. M. de; RUSCHEL, A. R.; CARVALHO, J. O. P. de; SILVA, J. N. M.; POORTER, L.; PEÑA-CLAROS, M.; BAUHUS, J. Effects of disturbance intensity and tree diversity on the biomass recovery of a managed tropical forest. In: ANNUAL MEETING OF THE ASSOCIATION FOR TROPICAL BIOLOGY AND CONSERVATION, 53., 2016, Montpellier. Tropical ecology and society: reconciling conservation and sustainable use of biodiversity: program & abstracts. [S.l.]: ATBC, 2016. p. 312.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 8. | | AVILA, A. L. de; SCHWARTZ, G.; RUSCHEL, A. R.; LOPES, J. do C.; SILVA, J. N. M.; CARVALHO, J. O. P. de; DORMANN, C. F.; MAZZEI, L.; SOARES, M. H. M.; BAUHUS, J. Recruitment, growth and recovery of commercial tree species over 30 years following logging and thinning in a tropical rain forest. Forest Ecology and Management, v. 385, p. 225-235, Feb. 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 9. | | AVILA, A. L. de; SANDE, M. T. van der; DORMANN, C. F.; PEÑA-CLAROS, M.; POORTER, L.; FREITAS, L. J. M. de; RUSCHEL, A. R.; SILVA, J. N. M.; CARVALHO, J. O. P. de; BAUHUS, J. Disturbance intensity is a stronger driver of biomass recovery than remaining tree-community attributes in a managed Amazonian forest. Journal of Applied Ecology, v. 55, n. 4, p. 1647-1657, July 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 10. | | AVILA, A. L. de; RUSCHEL, A. R.; CARVALHO, J. O. P. de; MAZZEI, L.; SILVA, J. N. M.; LOPES, J. do C.; ARAUJO, M. M.; DORMANN, C. F.; BAUHUS, J. Medium-term dynamics of tree species composition in response to silvicultural intervention intensities in a tropical rain forest. Biological Conservation, v. 191, p. 577-586, Nov. 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 11. | | SANDE, M. T. van der; ARETS, E. J. M. M.; PEÑA-CLAROS, M.; AVILA, A. L. de; ROOPSIND, A.; MAZZEI, L.; ASCARRUNZ, N.; FINEGAN, B.; ALARCÓN, A.; CÁCERES-SIANI, A.; LICONA, J. C.; RUSCHEL, A.; TOLEDO, M.; POORTER, L. Old-growth Neotropical forests are shifting in species and trait composition. Ecological Monographs, v. 86, n. 2, p. 228-243, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| Registros recuperados : 11 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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