| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Soja. |
|
Data corrente: |
05/01/2012 |
|
Data da última atualização: |
19/07/2024 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
KULCHESKI, F. R.; OLIVEIRA, L. F. V.; MOLINA, L. G.; ALMERAO, M. P.; RODRIGUES, F. A.; MARCOLINO, J.; BARBOSA, J. F.; STOLF-MOREIRA, R.; NEPOMUCENO, A. L.; MARCELINO-GUIMARÃES, F. C.; ABDELNOOR, R. V.; NASCIMENTO, L. C.; CARAZZOLLE, M. F.; PEREIRA, G. A. G.; MARGIS, R. |
|
Afiliação: |
FRANCELI R. KULCHESKI, UFRGS; LUIZ F. V. DE OLIVEIRA, UFRGS; LORRAYNE G. MOLINA, UFRGS; MAURÍCIO P. ALMERÃO, UFRGS; FABIANA A. RODRIGUES; JULIANA MARCOLINO; JOICE F. BARBOSA; RENATA STOLF MOREIRA; ALEXANDRE LIMA NEPOMUCENO, SRI; FRANCISMAR CORREA MARCELINO GUIMARÃES, CNPSO; RICARDO VILELA ABDELNOOR, CNPSO; LEANDRO C. NASCIMENTO, UNICAMP; MARCELO F. CARAZZOLLE, UNICAMP; GONÇALO A. G. PEREIRA, UNICAMP; ROGÉRIO MARGIS, UFRGS. |
|
Título: |
Identification of novel soybean microRNAs involved in abiotic and biotic stresses. |
|
Ano de publicação: |
2011 |
|
Fonte/Imprenta: |
BMC Genomics, v. 12, article 307, jun. 2011. |
|
Páginas: |
17 p. |
|
DOI: |
10.1186/1471-2164-12-307 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Background: Small RNAs (19-24 nt) are key regulators of gene expression that guide both transcriptional and posttranscriptional silencing mechanisms in eukaryotes. Current studies have demonstrated that microRNAs (miRNAs) act in several plant pathways associated with tissue proliferation, differentiation, and development and in response to abiotic and biotic stresses. In order to identify new miRNAs in soybean and to verify those that are possibly water deficit and rust-stress regulated, eight libraries of small RNAs were constructed and submitted to Solexa sequencing. Results: The libraries were developed from drought-sensitive and tolerant seedlings and rust-susceptible and resistant soybeans with or without stressors. Sequencing the library and subsequent analyses detected 256 miRNAs. From this total, we identified 24 families of novel miRNAs that had not been reported before, six families of conserved miRNAs that exist in other plants species, and 22 families previously reported in soybean. We also observed the presence of several isomiRNAs during our analyses. To validate novel miRNAs, we performed RT-qPCR across the eight different libraries. Among the 11 miRNAs analyzed, all showed different expression profiles during biotic and abiotic stresses to soybean. The majority of miRNAs were up-regulated during water deficit stress in the sensitive plants. However, for the tolerant genotype, most of the miRNAs were down regulated. The pattern of miRNAs expression was also different for the distinct genotypes submitted to the pathogen stress. Most miRNAs were down regulated during the fungus infection in the susceptible genotype; however, in the resistant genotype, most miRNAs did not vary during rust attack. A prediction of the putative targets was carried out for conserved and novel miRNAs families. Conclusions: Validation of our results with quantitative RT-qPCR revealed that Solexa sequencing is a powerful tool for miRNA discovery. The identification of differentially expressed plant miRNAs provides molecular evidence for the possible involvement of miRNAs in the process of water deficit- and rust-stress responses. MenosBackground: Small RNAs (19-24 nt) are key regulators of gene expression that guide both transcriptional and posttranscriptional silencing mechanisms in eukaryotes. Current studies have demonstrated that microRNAs (miRNAs) act in several plant pathways associated with tissue proliferation, differentiation, and development and in response to abiotic and biotic stresses. In order to identify new miRNAs in soybean and to verify those that are possibly water deficit and rust-stress regulated, eight libraries of small RNAs were constructed and submitted to Solexa sequencing. Results: The libraries were developed from drought-sensitive and tolerant seedlings and rust-susceptible and resistant soybeans with or without stressors. Sequencing the library and subsequent analyses detected 256 miRNAs. From this total, we identified 24 families of novel miRNAs that had not been reported before, six families of conserved miRNAs that exist in other plants species, and 22 families previously reported in soybean. We also observed the presence of several isomiRNAs during our analyses. To validate novel miRNAs, we performed RT-qPCR across the eight different libraries. Among the 11 miRNAs analyzed, all showed different expression profiles during biotic and abiotic stresses to soybean. The majority of miRNAs were up-regulated during water deficit stress in the sensitive plants. However, for the tolerant genotype, most of the miRNAs were down regulated. The pattern of miRNAs expression was also di... Mostrar Tudo |
|
Thesagro: |
Deficiência hídrica; Gene marcador; Soja. |
|
Thesaurus Nal: |
Gene expression; Soil water deficit; Soybeans. |
|
Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
|
Marc: |
LEADER 03244naa a2200385 a 4500
001 1911802
005 2024-07-19
008 2011 bl uuuu u00u1 u #d
024 7 $a10.1186/1471-2164-12-307$2DOI
100 1 $aKULCHESKI, F. R.
245 $aIdentification of novel soybean microRNAs involved in abiotic and biotic stresses.$h[electronic resource]
260 $c2011
300 $a17 p.
520 $aBackground: Small RNAs (19-24 nt) are key regulators of gene expression that guide both transcriptional and posttranscriptional silencing mechanisms in eukaryotes. Current studies have demonstrated that microRNAs (miRNAs) act in several plant pathways associated with tissue proliferation, differentiation, and development and in response to abiotic and biotic stresses. In order to identify new miRNAs in soybean and to verify those that are possibly water deficit and rust-stress regulated, eight libraries of small RNAs were constructed and submitted to Solexa sequencing. Results: The libraries were developed from drought-sensitive and tolerant seedlings and rust-susceptible and resistant soybeans with or without stressors. Sequencing the library and subsequent analyses detected 256 miRNAs. From this total, we identified 24 families of novel miRNAs that had not been reported before, six families of conserved miRNAs that exist in other plants species, and 22 families previously reported in soybean. We also observed the presence of several isomiRNAs during our analyses. To validate novel miRNAs, we performed RT-qPCR across the eight different libraries. Among the 11 miRNAs analyzed, all showed different expression profiles during biotic and abiotic stresses to soybean. The majority of miRNAs were up-regulated during water deficit stress in the sensitive plants. However, for the tolerant genotype, most of the miRNAs were down regulated. The pattern of miRNAs expression was also different for the distinct genotypes submitted to the pathogen stress. Most miRNAs were down regulated during the fungus infection in the susceptible genotype; however, in the resistant genotype, most miRNAs did not vary during rust attack. A prediction of the putative targets was carried out for conserved and novel miRNAs families. Conclusions: Validation of our results with quantitative RT-qPCR revealed that Solexa sequencing is a powerful tool for miRNA discovery. The identification of differentially expressed plant miRNAs provides molecular evidence for the possible involvement of miRNAs in the process of water deficit- and rust-stress responses.
650 $aGene expression
650 $aSoil water deficit
650 $aSoybeans
650 $aDeficiência hídrica
650 $aGene marcador
650 $aSoja
700 1 $aOLIVEIRA, L. F. V.
700 1 $aMOLINA, L. G.
700 1 $aALMERAO, M. P.
700 1 $aRODRIGUES, F. A.
700 1 $aMARCOLINO, J.
700 1 $aBARBOSA, J. F.
700 1 $aSTOLF-MOREIRA, R.
700 1 $aNEPOMUCENO, A. L.
700 1 $aMARCELINO-GUIMARÃES, F. C.
700 1 $aABDELNOOR, R. V.
700 1 $aNASCIMENTO, L. C.
700 1 $aCARAZZOLLE, M. F.
700 1 $aPEREIRA, G. A. G.
700 1 $aMARGIS, R.
773 $tBMC Genomics$gv. 12, article 307, jun. 2011.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Soja (CNPSO) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 10 | |
| 1. |  | SILVA, M. R. da; OLIVEIRA, M. do S. P. de; MOURA, E. F.; RODRIGUES, S. de M. Seleção de primers ISSR para uso em genomas de camucamuzeiro. In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA AMAZÔNIA ORIENTAL, 17.; SEMINÁRIO DE PÓS-GRADUAÇÃO DA EMBRAPA AMAZÔNIA ORIENTAL, 1., 2013, Belém, PA. Anais. Belém, PA: Embrapa Amazônia Oriental, 2013. 1 CD-ROM. PIBIC 2013.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
|    |
| 6. | | FERREIRA, J.; BRIENZA JUNIOR, S.; BATISTELLA, M.; ABREU, L. S. de; CELESTINO, P.; MATTOS, L. M. de; XAUD, M. R.; SILVA, M. R. da; BUSCHINELLI, C. C. de A.; PINTO, E. de P.; MOUTINHO, P.; STACHETTI, G. R. Agroambiente network: ecosystem services in agricultural landscapes in the Brazilian Amazon. In: SEMINARIO INTERNACIONAL ADAPTACIÓN AL CAMBIO CLIMÁTICO: EL ROL DE LOS SERVICIOS ECOSISTÉMICOS, 2008, Turrialba. Resúmenes... Turrialba: CATIE; Belém, PA: CIFOR, 2008. p. 94. Título do evento em Inglês: INTERNATIONAL WORKSHOP "ADAPTATION FOR CLIMATE CHANGE: THE ROLE OF ECOSYSTEM SERVICES, 2008, Turrialba".| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
| |
| 7. | | FERREIRA, J. N.; BRIENZA JUNIOR, S.; BATISTELLA, M.; ABREU, L. S. de; CELESTINO FILHO, P.; MATTOS, L. M. de; XAUD, M. R.; SILVA, M. R. da; BUSCHINELLI, C. C. de A.; PINTO, E. de P.; MOUTINHO, P.; STACHETTI, G. Agroambiente network: ecosystem services in agricultural landscapes in the Brazilian Amazon. In: ACES: A CONFERENCE ON ECOSYSTEM SERVICES, 2 USING SCIENCE FOR DECISION MAKING IN DYNAMIC SYSTEMS, Naples, FL. Abstract book... Gainesville: University of Florida, 2008. p. 46.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental; Embrapa Territorial. |
| |
| 8. | | SANCHES, M. M.; WULFF, N. A.; SILVA, M. R. L.; LEITE JUNIOR, R. P.; FERREIRA, E. A.; SANTOS, J. F. dos; LOPES SILVA, M.; ANGARTEN, M. B. de O.; ISHIDA, A. K. N.; OLIVEIRA, R. P. de; LUCCAS, B. E. G. de; CARBONARI, J. J.; MARTINS, O. M. Levantamento de huanglongbing (HLB) em citros no Brasil e diagnose dos agentes etiológicos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2018. 21 p. (Embrapa Recursos Genéticos e Biotecnologia. Boletim de pesquisa e desenvolvimento, 337).| Tipo: Boletim de Pesquisa e Desenvolvimento |
| Biblioteca(s): Embrapa Amazônia Oriental; Embrapa Clima Temperado; Embrapa Recursos Genéticos e Biotecnologia. |
| |
| 9. | | GATO, R. F.; MATOS, L. M. S. de; AZEVEDO, C. M. B. C. de; CERQUEIRA, J. G.; CERQUEIRA SEGUNDO, E. F.; VASCONCELOS, M. A. M.; SOUZA, V. B.; PINHEIRO, L. V. I.; SILVA, M. R. da; SANTOS, F. C. dos; POPA, R. R.; FERREIRA, J. H. O. Usando a ciência para transformar paisagens: divulgação de práticas sustentáveis para a recuperação de áreas degradadas. In: CONFERÊNCIA DO SUBPROGRAMA DE CIÊNCIA E TECNOLOGIA - SPC&T FASE II/PPG7, 2008, Belém, PA. Anais... Brasília, DF: CNPq, 2009. p. 560-566. Disponível também em CD-ROM (CD 00173).| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Amazônia Oriental. |
| |
| 10. | | CASTILHOS, D.; POLESSO, A. M.; SILVA, A. C. F. da; SANTOS, A. B. dos; LOPES, A. T. de C.; OLIVEIRA FILHO, A. A. de; CUNHA, A. F. S. da; PUKER, A.; MATIOLI, A. L.; CAMPOS, A. A. S.; MORAIS, A. L. de; MODRO, A. F. H.; SILVEIRA NETO, A. A.; PIRES, A. de S. M.; BIASI, C.; PEIXOTO, C. M.; MERCÊS, C. da C.; CARVALHO, C. A. L. de; PAIVA, C. da S.; PEREIRA, D. S.; FREIRE, D. S.; SOUZA, D. C.; JONG, D. de; SOARES, D. A. M.; ARAÚJO NETO, E. R. de; SOUZA, E. A. de; SANTOS, E. B.; MAIA, E.; ARAÚJO, E. A. B. de; LIMA, E. G.; TEIXEIRA, E. W.; NASCIMENTO, E. G.; SILVA, F. A. S. e; CAPPELARI, F. A.; CHAGAS SOBRINHO, F.; FONTES, F. M.; OLINTO, F. A.; SILVA, F. E. J. da; BERGAMO, G. C.; RODRIGUES, G. A.; MORETTO, G.; CASADIO, G. M. L.; BOGUS, G. M.; CARVALHO, G. G. P. de; OLIVEIRA NETO, G. L. de; CASTAGNINO, G.; QUEIROZ, G. S. de; PIRES, G. P.; SILVA, H. M. da; SILVA NETO, H. B.; NUNES, H. T. de S.; OLIVEIRA, I. P. P.; SAMBRANA, I. R.; ROCHA, J. S.; BANDERÓ, J. L.; ALBUQUERQUE, J. G. S. S. de; HOLANDA NETO, J. P. de; KASTELIC, J.; GUERRA JUNIOR, J. C. V.; COSTA, J. R. da; JESUS, J. S. de; BENDINI, J.; GRAMACHO, K. P.; SOUZA, L.; LEONI, L. N.; GONÇALVES, L. S.; CESTARO, L. G.; MORAIS, L. da S.; DANTAS, L. F. C. F.; SERRA, L. S.; ABREU, L. de; BORSUK, L. C.; PINTO, L. F. B.; SILVA, L. A. da; LIMA, L. A. dos A.; FOGAÇA, M. J.; ISSA, M. R. C.; CORREIA-OLIVEIRA, M. E.; ALVES, M. L. T. M. F.; BARRETO, M. R.; REY, M. C. del; BARBOSA, M. J. de O. C.; SILVA, M. R. da; MORAIS, M. M.; VINHAL-SILVA, N.; GOMES, P. T. de O.; GUIMARÃES-BEELEN, P. M.; SOUSA, P. O. de; COSTA, P. S. C.; SOUZA, P. A. de; PAULA, R. de; MENDES, R. B.; GOMES, R. V. R. de S.; SANTOS, R. G.; NASCIMENTO, R. J. T.; SILVEIRA, R. D.; CABRAL, R. S.; TORRES, R.; BEELEN, R.; PEREIRA, R. A.; RIZZARDO, R. A. G.; CUNHA, S. A.; CELMER, S. T.; ESTEVAN, S.; ASSIS JUNIOR, S. L. de; MACIEL, S. V. S. A.; SILVA, S. J. R. da; MOREIRA, S.; SCHAFASCHEK, T. P.; BARROS, T. A. de; RUELA, T. H.; VITENA, T. M. de S.; BRIDI, T. R.; LEITE, V. M.; REIS, V. D. A. dos; NEVES, V. S. L. das; MEDEIROS, V. F. de. Varroa destructor infestation levels in Africanized honey bee colonies in Brazil from 1977 when first detected to 2020. Apidologie, v. 54, Article 5, 2023.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Amazônia Oriental. |
|  |
| Registros recuperados : 10 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
