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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
03/10/2024 |
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Data da última atualização: |
03/10/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
LEME, L. de O.; CARVALHO, J. O.; MENDES, C. M.; ASSUMPÇÃO, M. E. O. D.; CAETANO, A. R.; FRANCO, M. M.; DODE, M. A. N. |
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Afiliação: |
LIGIANE DE OLIVEIRA LEME, UFES, UNIVERSIDADE FEDERAL DO ESPÍRITO SANTO; JOSÉ OLIVEIRA CARVALHO, UFES, UNIVERSIDADE FEDERAL DO ESPÍRITO SANTO; CAMILLA MOTTA MENDES, USP, UNIVERSIDADE DE SÃO PAULO; MAYRA ELENA ORTIZ D'AVILA ASSUMPÇÃO, USP, UNIVERSIDADE DE SÃO PAULO; ALEXANDRE RODRIGUES CAETANO, CENARGEN; MAURICIO MACHAIM FRANCO, CENARGEN; MARGOT ALVES NUNES DODE, CENARGEN. |
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Título: |
Impact of sperm sex sorting on sperm quality and in vitro embryo production in bovine. |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
Animal Reproduction Science, v. 270, 107604, 2024. |
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DOI: |
https://doi.org/10.1016/j.anireprosci.2024.107604 |
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Idioma: |
Inglês |
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Conteúdo: |
Increasing evidence suggests that environmental exposures can modify epigenetic marks in the germline, leading to the transmission of abnormal post-fertilization sperm epigenetic indicators and affecting embryonic development. Given the pivotal role of sperm cells in determining embryo quality, there is growing interest in understanding the potential effects of sperm sex sorting on embryo quality. This study aimed to investigate the impact of bovine sperm sexing on in vitro embryo production (IVP) and to associate molecular aspects of embryos analysis. Frozen semen samples from five Nellore bulls were used, with each bull contributing unsexed sperm (conventional semen – CV treatment) and female and male sexed sperm pooled after thawing (SX treatment). First, semen quality was assessed, including motility, morphology, acrosome integrity, and chromatin integrity to denaturation. Then, IVP was carried out, focusing on embryonic production and developmental kinetics. In the third experiment, embryo quality was evaluated by examining the gene expression of key markers (OCT4, NANOG, DNMT3A, TET1, and Fematrin-1) and the methylation pattern of the Satellite-1 and α-Satellite genes in blastocysts. Differences between CV and SX semen were only observed in motility, which was lower in SX compared with CV (P < 0.05). Although cleavage was similar, the SX groups showed lower blastocyst production than CV (P < 0.05). Of the genes evaluated, only NANOG showed high expression in the CV blastocysts compared with the SX blastocysts, but the methylation pattern revealed no differences. In conclusion, sex sorting markedly affects sperm motility and in vitro embryo production but showed no significant impact on embryo quality. MenosIncreasing evidence suggests that environmental exposures can modify epigenetic marks in the germline, leading to the transmission of abnormal post-fertilization sperm epigenetic indicators and affecting embryonic development. Given the pivotal role of sperm cells in determining embryo quality, there is growing interest in understanding the potential effects of sperm sex sorting on embryo quality. This study aimed to investigate the impact of bovine sperm sexing on in vitro embryo production (IVP) and to associate molecular aspects of embryos analysis. Frozen semen samples from five Nellore bulls were used, with each bull contributing unsexed sperm (conventional semen – CV treatment) and female and male sexed sperm pooled after thawing (SX treatment). First, semen quality was assessed, including motility, morphology, acrosome integrity, and chromatin integrity to denaturation. Then, IVP was carried out, focusing on embryonic production and developmental kinetics. In the third experiment, embryo quality was evaluated by examining the gene expression of key markers (OCT4, NANOG, DNMT3A, TET1, and Fematrin-1) and the methylation pattern of the Satellite-1 and α-Satellite genes in blastocysts. Differences between CV and SX semen were only observed in motility, which was lower in SX compared with CV (P < 0.05). Although cleavage was similar, the SX groups showed lower blastocyst production than CV (P < 0.05). Of the genes evaluated, only NANOG showed high expression in the CV bla... Mostrar Tudo |
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Palavras-Chave: |
Embryo quality; Sex-sorting. |
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Thesaurus Nal: |
Cattle; Methylation. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02503naa a2200253 a 4500
001 2167760
005 2024-10-03
008 2024 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.anireprosci.2024.107604$2DOI
100 1 $aLEME, L. de O.
245 $aImpact of sperm sex sorting on sperm quality and in vitro embryo production in bovine.$h[electronic resource]
260 $c2024
520 $aIncreasing evidence suggests that environmental exposures can modify epigenetic marks in the germline, leading to the transmission of abnormal post-fertilization sperm epigenetic indicators and affecting embryonic development. Given the pivotal role of sperm cells in determining embryo quality, there is growing interest in understanding the potential effects of sperm sex sorting on embryo quality. This study aimed to investigate the impact of bovine sperm sexing on in vitro embryo production (IVP) and to associate molecular aspects of embryos analysis. Frozen semen samples from five Nellore bulls were used, with each bull contributing unsexed sperm (conventional semen – CV treatment) and female and male sexed sperm pooled after thawing (SX treatment). First, semen quality was assessed, including motility, morphology, acrosome integrity, and chromatin integrity to denaturation. Then, IVP was carried out, focusing on embryonic production and developmental kinetics. In the third experiment, embryo quality was evaluated by examining the gene expression of key markers (OCT4, NANOG, DNMT3A, TET1, and Fematrin-1) and the methylation pattern of the Satellite-1 and α-Satellite genes in blastocysts. Differences between CV and SX semen were only observed in motility, which was lower in SX compared with CV (P < 0.05). Although cleavage was similar, the SX groups showed lower blastocyst production than CV (P < 0.05). Of the genes evaluated, only NANOG showed high expression in the CV blastocysts compared with the SX blastocysts, but the methylation pattern revealed no differences. In conclusion, sex sorting markedly affects sperm motility and in vitro embryo production but showed no significant impact on embryo quality.
650 $aCattle
650 $aMethylation
653 $aEmbryo quality
653 $aSex-sorting
700 1 $aCARVALHO, J. O.
700 1 $aMENDES, C. M.
700 1 $aASSUMPÇÃO, M. E. O. D.
700 1 $aCAETANO, A. R.
700 1 $aFRANCO, M. M.
700 1 $aDODE, M. A. N.
773 $tAnimal Reproduction Science$gv. 270, 107604, 2024.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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