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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura; Embrapa Soja. |
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Data corrente: |
26/08/2024 |
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Data da última atualização: |
27/08/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
NASCIMENTO, F. dos S.; MASCARENHAS, M. S.; BOAVENTURA, S. C.; SOUZA, C. C. H. de; RAMOS, A. P. de S.; ROCHA, A. de J.; SOARES, J. M. da S.; DINIZ, L. E. C.; MENDES, T. A. de O.; FERREIRA, C. F.; AMORIM, E. P. |
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Afiliação: |
FERNANDA DOS SANTOS NASCIMENTO, STATE UNIVERSITY OF FEIRA DE SANTANA; MARCELLY SANTANA MASCARENHAS, STATE UNIVERSITY OF FEIRA DE SANTANA; SAMANTHA COSTA BOAVENTURA, FEDERAL UNIVERSITY OF RECÔNCAVO DA BAHIA; CARLA CATHARINA HORA DE SOUZA, FEDERAL UNIVERSITY OF RECÔNCAVO DA BAHIA; ANDRESA PRISCILA DE SOUZA RAMOS, CNPMF; ANELITA DE JESUS ROCHA, STATE UNIVERSITY OF FEIRA DE SANTANA; JULIANNA MATOS DA SILVA SOARES, STATE UNIVERSITY OF FEIRA DE SANTANA; LEANDRO EUGENIO CARDAMONE DINIZ, CNPSO; TIAGO ANTÔNIO DE OLIVEIRA MENDES, FEDERAL UNIVERSITY OF VIÇOSA; CLAUDIA FORTES FERREIRA, CNPMF; EDSON PERITO AMORIM, CNPMF. |
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Título: |
Phytoene Desaturase (PDS) Gene-Derived Markers Identify “A” and “B” Genomes in Banana (Musa spp.). |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
Horticulturae, v. 10, n. 3, 294, 2024. |
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Páginas: |
11 p. |
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ISSN: |
2311-7524 |
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DOI: |
10.3390/horticulturae10030294 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract Phytoene desaturase (PDS) is a plant enzyme involved in carotenoid biosynthesis. The PDS gene has been used as a selective marker for genome editing in several plant species, including banana (Musa spp.). Its knockout promotes dwarfism and albinism, characteristics that are easily recognizable and highly favorable. In Musa spp., the A genome increases fruit production and quality, whereas the B genome is associated with tolerance to biotic and abiotic stresses. The objective of this study was to identify a molecular marker in the PDS gene to easily discriminate the A and B genomes of banana. A 2166 bp fragment for the “PDSMa” marker was identified as polymorphic for the A genome (identification accuracy of 99.33%), whereas ~332 and ~225 bp fragments were detected for the “PDSMb” marker with 100% accuracy using MedCalc software. In this study, we used genotypes with A and B genomes that are used in the genetic improvement of bananas and an accession with the BT genome. It was not possible to differentiate the accession with the BT genome from the others, suggesting that the markers do not have the capacity to separate the T genome from the A and B genomes. To the best of our knowledge, this is the first study to use the PDS gene to determine doses of the A genome and identify the B genome in Musa spp., which will aid in evaluating the genomic constitution of banana hybrids and accessions at the seedling stage and accelerating their classification in crop genetic improvement programs. MenosAbstract Phytoene desaturase (PDS) is a plant enzyme involved in carotenoid biosynthesis. The PDS gene has been used as a selective marker for genome editing in several plant species, including banana (Musa spp.). Its knockout promotes dwarfism and albinism, characteristics that are easily recognizable and highly favorable. In Musa spp., the A genome increases fruit production and quality, whereas the B genome is associated with tolerance to biotic and abiotic stresses. The objective of this study was to identify a molecular marker in the PDS gene to easily discriminate the A and B genomes of banana. A 2166 bp fragment for the “PDSMa” marker was identified as polymorphic for the A genome (identification accuracy of 99.33%), whereas ~332 and ~225 bp fragments were detected for the “PDSMb” marker with 100% accuracy using MedCalc software. In this study, we used genotypes with A and B genomes that are used in the genetic improvement of bananas and an accession with the BT genome. It was not possible to differentiate the accession with the BT genome from the others, suggesting that the markers do not have the capacity to separate the T genome from the A and B genomes. To the best of our knowledge, this is the first study to use the PDS gene to determine doses of the A genome and identify the B genome in Musa spp., which will aid in evaluating the genomic constitution of banana hybrids and accessions at the seedling stage and accelerating their classification in crop genetic impr... Mostrar Tudo |
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Thesagro: |
Banana; Genética; Genética Vegetal. |
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Thesaurus Nal: |
Genes; Musa. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
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024 7 $a10.3390/horticulturae10030294$2DOI
100 1 $aNASCIMENTO, F. dos S.
245 $aPhytoene Desaturase (PDS) Gene-Derived Markers Identify “A” and “B” Genomes in Banana (Musa spp.).$h[electronic resource]
260 $c2024
300 $a11 p.
520 $aAbstract Phytoene desaturase (PDS) is a plant enzyme involved in carotenoid biosynthesis. The PDS gene has been used as a selective marker for genome editing in several plant species, including banana (Musa spp.). Its knockout promotes dwarfism and albinism, characteristics that are easily recognizable and highly favorable. In Musa spp., the A genome increases fruit production and quality, whereas the B genome is associated with tolerance to biotic and abiotic stresses. The objective of this study was to identify a molecular marker in the PDS gene to easily discriminate the A and B genomes of banana. A 2166 bp fragment for the “PDSMa” marker was identified as polymorphic for the A genome (identification accuracy of 99.33%), whereas ~332 and ~225 bp fragments were detected for the “PDSMb” marker with 100% accuracy using MedCalc software. In this study, we used genotypes with A and B genomes that are used in the genetic improvement of bananas and an accession with the BT genome. It was not possible to differentiate the accession with the BT genome from the others, suggesting that the markers do not have the capacity to separate the T genome from the A and B genomes. To the best of our knowledge, this is the first study to use the PDS gene to determine doses of the A genome and identify the B genome in Musa spp., which will aid in evaluating the genomic constitution of banana hybrids and accessions at the seedling stage and accelerating their classification in crop genetic improvement programs.
650 $aGenes
650 $aMusa
650 $aBanana
650 $aGenética
650 $aGenética Vegetal
700 1 $aMASCARENHAS, M. S.
700 1 $aBOAVENTURA, S. C.
700 1 $aSOUZA, C. C. H. de
700 1 $aRAMOS, A. P. de S.
700 1 $aROCHA, A. de J.
700 1 $aSOARES, J. M. da S.
700 1 $aDINIZ, L. E. C.
700 1 $aMENDES, T. A. de O.
700 1 $aFERREIRA, C. F.
700 1 $aAMORIM, E. P.
773 $tHorticulturae$gv. 10, n. 3, 294, 2024.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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