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Registro Completo |
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
25/02/2015 |
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Data da última atualização: |
25/02/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
VAZ, C. S. L.; RECH, D. V.; POZZA, J. dos S.; COLDEBELLA, A.; SILVA, V. S. |
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Afiliação: |
CLARISSA SILVEIRA LUIZ VAZ, CNPSA; DAIANE VOSS RECH, CNPSA; JENIFER DOS SANTOS POZZA, UNC; ARLEI COLDEBELLA, CNPSA; VIRGINIA SANTIAGO SILVA, CNPSA. |
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Título: |
Isolation of campylobacter from brazilian broiler flocks using different culturing procedures. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Poultry Science, v. 93, p. 1-6, 2014. |
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DOI: |
10.3382/ps.2014-03943 |
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Idioma: |
Inglês |
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Conteúdo: |
Conventional culturing methods enable the detection of Campylobacter in broiler flocks. However, laboratory culture of Campylobacter is laborious because of its fastidious behavior and the presence of competing nontarget bacteria. This study evaluated different protocols to isolate Campylobacter from broiler litter, feces, and cloacal and drag swabs. Samples taken from commercial Brazilian broiler flocks were directly streaked onto Preston agar (PA), Campy-Line agar (CLA), and modified charcoal cefoperazone deoxycholate agar (mCCDA) and also enriched in blood-free Bolton broth (bfBB) for 24 and 48 h followed by plating onto the different selective media. Higher numbers of Campylobacter-positive cloacal and drag swab samples were observed using either direct plating or enrichment for 24 h before plating onto PA, compared with enrichment for 48 h (P < 0.05). Furthermore, direct plating was a more sensitive method to detect Campylobacter in broiler litter and feces samples. Analysis of directly plated samples revealed that higher Campylobacter levels were detected in feces streaked onto PA (88.8%), cloacal swabs plated onto mCCDA (72.2%), drag swabs streaked onto CLA or mCCDA (69.4%), and litter samples inoculated onto PA (63.8%). Preston agar was the best agar to isolate Campylobacter from directly plated litter samples (P < 0.05), but there was no difference in the efficacies of PA, mCCDA, and CLA in detecting Campylobacter in other samples. The isolated Campylobacter strains were phenotypically identified as Campylobacter jejuni or Campylobacter coli. The predominant contaminant observed in the Campylobacter cultures was Proteus mirabilis, which was resistant to the majority of antimicrobial agents in selective media. Together, these data showed that direct plating onto PA and onto either CLA or mCCDA as the second selective agar enabled the reliable isolation of thermophilic Campylobacter species from broiler samples. Finally, Campylobacter was detected in all broiler flocks sampled. MenosConventional culturing methods enable the detection of Campylobacter in broiler flocks. However, laboratory culture of Campylobacter is laborious because of its fastidious behavior and the presence of competing nontarget bacteria. This study evaluated different protocols to isolate Campylobacter from broiler litter, feces, and cloacal and drag swabs. Samples taken from commercial Brazilian broiler flocks were directly streaked onto Preston agar (PA), Campy-Line agar (CLA), and modified charcoal cefoperazone deoxycholate agar (mCCDA) and also enriched in blood-free Bolton broth (bfBB) for 24 and 48 h followed by plating onto the different selective media. Higher numbers of Campylobacter-positive cloacal and drag swab samples were observed using either direct plating or enrichment for 24 h before plating onto PA, compared with enrichment for 48 h (P < 0.05). Furthermore, direct plating was a more sensitive method to detect Campylobacter in broiler litter and feces samples. Analysis of directly plated samples revealed that higher Campylobacter levels were detected in feces streaked onto PA (88.8%), cloacal swabs plated onto mCCDA (72.2%), drag swabs streaked onto CLA or mCCDA (69.4%), and litter samples inoculated onto PA (63.8%). Preston agar was the best agar to isolate Campylobacter from directly plated litter samples (P < 0.05), but there was no difference in the efficacies of PA, mCCDA, and CLA in detecting Campylobacter in other samples. The isolated Campylobacter strains... Mostrar Tudo |
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Thesagro: |
Campilobacteriose; Frango de corte; Segurança alimentar. |
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Thesaurus Nal: |
Bacteriology; Broiler chickens; Campylobacter coli; Campylobacter jejuni; Food contamination; Food safety. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02865naa a2200289 a 4500
001 2009812
005 2015-02-25
008 2014 bl uuuu u00u1 u #d
024 7 $a10.3382/ps.2014-03943$2DOI
100 1 $aVAZ, C. S. L.
245 $aIsolation of campylobacter from brazilian broiler flocks using different culturing procedures.$h[electronic resource]
260 $c2014
520 $aConventional culturing methods enable the detection of Campylobacter in broiler flocks. However, laboratory culture of Campylobacter is laborious because of its fastidious behavior and the presence of competing nontarget bacteria. This study evaluated different protocols to isolate Campylobacter from broiler litter, feces, and cloacal and drag swabs. Samples taken from commercial Brazilian broiler flocks were directly streaked onto Preston agar (PA), Campy-Line agar (CLA), and modified charcoal cefoperazone deoxycholate agar (mCCDA) and also enriched in blood-free Bolton broth (bfBB) for 24 and 48 h followed by plating onto the different selective media. Higher numbers of Campylobacter-positive cloacal and drag swab samples were observed using either direct plating or enrichment for 24 h before plating onto PA, compared with enrichment for 48 h (P < 0.05). Furthermore, direct plating was a more sensitive method to detect Campylobacter in broiler litter and feces samples. Analysis of directly plated samples revealed that higher Campylobacter levels were detected in feces streaked onto PA (88.8%), cloacal swabs plated onto mCCDA (72.2%), drag swabs streaked onto CLA or mCCDA (69.4%), and litter samples inoculated onto PA (63.8%). Preston agar was the best agar to isolate Campylobacter from directly plated litter samples (P < 0.05), but there was no difference in the efficacies of PA, mCCDA, and CLA in detecting Campylobacter in other samples. The isolated Campylobacter strains were phenotypically identified as Campylobacter jejuni or Campylobacter coli. The predominant contaminant observed in the Campylobacter cultures was Proteus mirabilis, which was resistant to the majority of antimicrobial agents in selective media. Together, these data showed that direct plating onto PA and onto either CLA or mCCDA as the second selective agar enabled the reliable isolation of thermophilic Campylobacter species from broiler samples. Finally, Campylobacter was detected in all broiler flocks sampled.
650 $aBacteriology
650 $aBroiler chickens
650 $aCampylobacter coli
650 $aCampylobacter jejuni
650 $aFood contamination
650 $aFood safety
650 $aCampilobacteriose
650 $aFrango de corte
650 $aSegurança alimentar
700 1 $aRECH, D. V.
700 1 $aPOZZA, J. dos S.
700 1 $aCOLDEBELLA, A.
700 1 $aSILVA, V. S.
773 $tPoultry Science$gv. 93, p. 1-6, 2014.
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Registro original: |
Embrapa Suínos e Aves (CNPSA) |
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