| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
|
Data corrente: |
14/05/1998 |
|
Data da última atualização: |
24/07/2023 |
|
Autoria: |
VAITUKAITIS, J.; ROBBINS, J. B.; NIESCHLAG, E.; ROSS, G. T. |
|
Título: |
A method for producing specific antisera with small doses of immunogen. |
|
Ano de publicação: |
1971 |
|
Fonte/Imprenta: |
Journal Clinical Endocrinology, v. 33, n. 6, p. 988-991, Dec. 1971. |
|
DOI: |
10.1210/jcem-33-6-988 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Abstract: We have succeeded in producing specific antisera to the subunits of HCG and testosterone conjugates with one immunization of 20 to 100 ?g of immunogen. The potential of the method for producing specific antisera to glycoprotein and other hormones with limited amounts of purified material prompts this report. Materials and Methods CR 100? and CR 100?, the subunits of HCG,. were prepared by Drs. R. E. Canfield and F. Morgan (1). Testosterone-3-(0-carboxymethy1) oxime was coupled to either bovine serum albumin (Armour) or keyhole limpet hemocyanin (Calbiochem) (2). Equal volumes of complete Freund's adjuvant, a 0.15 M NaCl solution of the immunogen and dried tubercle bacilli (2.5 mg/ml of total mixture) were homogenized for 5?10 minutes at increasing speeds to maximum setting in a Sorvall Omnimixer. Fur was shaved from the back and proximal limbs of 3 month old New Zealand white rabbits and the emulsion administered intradermally at 30?50 sites so that each animal received a total of 2 ml of the emulsion. At a separate site 0.5 ml of crude Bordetella pertussis vaccine (E. Lilly Corp.) was injected intradermally. |
|
Thesagro: |
Endocrinologia. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 01686naa a2200181 a 4500
001 1523906
005 2023-07-24
008 1971 bl uuuu u00u1 u #d
024 7 $a10.1210/jcem-33-6-988$2DOI
100 1 $aVAITUKAITIS, J.
245 $aA method for producing specific antisera with small doses of immunogen.$h[electronic resource]
260 $c1971
520 $aAbstract: We have succeeded in producing specific antisera to the subunits of HCG and testosterone conjugates with one immunization of 20 to 100 ?g of immunogen. The potential of the method for producing specific antisera to glycoprotein and other hormones with limited amounts of purified material prompts this report. Materials and Methods CR 100? and CR 100?, the subunits of HCG,. were prepared by Drs. R. E. Canfield and F. Morgan (1). Testosterone-3-(0-carboxymethy1) oxime was coupled to either bovine serum albumin (Armour) or keyhole limpet hemocyanin (Calbiochem) (2). Equal volumes of complete Freund's adjuvant, a 0.15 M NaCl solution of the immunogen and dried tubercle bacilli (2.5 mg/ml of total mixture) were homogenized for 5?10 minutes at increasing speeds to maximum setting in a Sorvall Omnimixer. Fur was shaved from the back and proximal limbs of 3 month old New Zealand white rabbits and the emulsion administered intradermally at 30?50 sites so that each animal received a total of 2 ml of the emulsion. At a separate site 0.5 ml of crude Bordetella pertussis vaccine (E. Lilly Corp.) was injected intradermally.
650 $aEndocrinologia
700 1 $aROBBINS, J. B.
700 1 $aNIESCHLAG, E.
700 1 $aROSS, G. T.
773 $tJournal Clinical Endocrinology$gv. 33, n. 6, p. 988-991, Dec. 1971.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 1 | |
| 1. |  | FERREIRA NETTO, T.; AMANCIO, C. O. da G.; UZEDA, M. C.; SILVA, L. I. da; OLIVEIRA, R. R. de. Sistematização de experiência e monitoramento das técnicas agroecológicas do assentamento de São José da Boa Morte, RJ. In: SEMANA CIENTÍFICA JOHANNA DÖBEREINER, 12., 2012. Economia verde, sustentabilidade e erradicação da pobreza: caderno de resumos. Seropédica: Embrapa Agrobiologia, 2012.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
|    |
| Registros recuperados : 1 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
