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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
29/01/2009 |
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Data da última atualização: |
24/05/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
WOLFF, J. L.; VALICENTE, F. H.; MARTINS, R.; OLIVEIRA, J. V. de C.; ZANOTTO, P. M. de A. |
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Afiliação: |
José Luiz Caldas Wolff, Universidade de Mogi das Cruzes; FERNANDO HERCOS VALICENTE, CNPMS; Renata Martins, Universidade de Mogi das Cruzes; Juliana Velasco de Castro Oliveira, USP; Paolo Marinho de andrade Zanotto, USP. |
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Título: |
Analysis of the genome of Spodoptera frugiperda nucleopolyhedrovirus (SfMNPV-19) and of the high genomic heterogeneity in group II nucleopolyhedroviruses. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
Journal of General Virology, Cambridge, v. 89, n. 6, p. 1202-1211, 2008. |
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DOI: |
10.1099/vir.0.83581-0 |
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Idioma: |
Inglês |
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Conteúdo: |
The genome of the most virulent among 22 Brazilian geographical isolates of Spodoptera Irugiperda nucleopolyhedrovirus, isolate 19 (SfMNPV-19), was completely sequenced and shown to comprise 132565 bp and 141 open reading frames (ORFs). A total of 11 ORFs with no homology to genes in the GenBank database were found. Of those, four had typical baculovirus promoter motifs and polyadenylation sites. Computer-simulated restriction enzyme cleavage patterns of SfMNPV-19 were compared with published physical maps of other SfMNPV isolates. Differences were observed in terms of the restriction profiles and genome size. Comparison of SfMNPV-19 with the sequence of the SfMNPV isolate 3AP2 indicated that they differed due to a 1427 bp deletion, as well as by a series of smaller deletions and point mutations. The majority of genes of SfMNPV-19 were conserved in the closely related Spodoptera exígua NPV (SeMNPV) and Agrotís segeturn NPV (AgseMNPV-A), but a few regions experienced major changes and rearrangements. Synthenic maps for the genomes of group 11 NPVs revealed that gene collinearity was observed only within certain clusters. Analysis of the dynamics of gene gain and loss along the phylogenetic tree of the NPVs showed that group 11 had only five defining genes and supported the hypothesis that these viruses form ten highly divergent ancient lineages. Crucially, more than 60 % of the gene gain events followed a power-Iaw relation to genetic distance among baculoviruses, indicative of temporal organization in the gene accretion processo. MenosThe genome of the most virulent among 22 Brazilian geographical isolates of Spodoptera Irugiperda nucleopolyhedrovirus, isolate 19 (SfMNPV-19), was completely sequenced and shown to comprise 132565 bp and 141 open reading frames (ORFs). A total of 11 ORFs with no homology to genes in the GenBank database were found. Of those, four had typical baculovirus promoter motifs and polyadenylation sites. Computer-simulated restriction enzyme cleavage patterns of SfMNPV-19 were compared with published physical maps of other SfMNPV isolates. Differences were observed in terms of the restriction profiles and genome size. Comparison of SfMNPV-19 with the sequence of the SfMNPV isolate 3AP2 indicated that they differed due to a 1427 bp deletion, as well as by a series of smaller deletions and point mutations. The majority of genes of SfMNPV-19 were conserved in the closely related Spodoptera exígua NPV (SeMNPV) and Agrotís segeturn NPV (AgseMNPV-A), but a few regions experienced major changes and rearrangements. Synthenic maps for the genomes of group 11 NPVs revealed that gene collinearity was observed only within certain clusters. Analysis of the dynamics of gene gain and loss along the phylogenetic tree of the NPVs showed that group 11 had only five defining genes and supported the hypothesis that these viruses form ten highly divergent ancient lineages. Crucially, more than 60 % of the gene gain events followed a power-Iaw relation to genetic distance among baculoviruses, indicative ... Mostrar Tudo |
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Thesagro: |
Praga de planta. |
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Categoria do assunto: |
O Insetos e Entomologia |
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Marc: |
LEADER 02240naa a2200193 a 4500
001 1491654
005 2018-05-24
008 2008 bl uuuu u00u1 u #d
024 7 $a10.1099/vir.0.83581-0$2DOI
100 1 $aWOLFF, J. L.
245 $aAnalysis of the genome of Spodoptera frugiperda nucleopolyhedrovirus (SfMNPV-19) and of the high genomic heterogeneity in group II nucleopolyhedroviruses.$h[electronic resource]
260 $c2008
520 $aThe genome of the most virulent among 22 Brazilian geographical isolates of Spodoptera Irugiperda nucleopolyhedrovirus, isolate 19 (SfMNPV-19), was completely sequenced and shown to comprise 132565 bp and 141 open reading frames (ORFs). A total of 11 ORFs with no homology to genes in the GenBank database were found. Of those, four had typical baculovirus promoter motifs and polyadenylation sites. Computer-simulated restriction enzyme cleavage patterns of SfMNPV-19 were compared with published physical maps of other SfMNPV isolates. Differences were observed in terms of the restriction profiles and genome size. Comparison of SfMNPV-19 with the sequence of the SfMNPV isolate 3AP2 indicated that they differed due to a 1427 bp deletion, as well as by a series of smaller deletions and point mutations. The majority of genes of SfMNPV-19 were conserved in the closely related Spodoptera exígua NPV (SeMNPV) and Agrotís segeturn NPV (AgseMNPV-A), but a few regions experienced major changes and rearrangements. Synthenic maps for the genomes of group 11 NPVs revealed that gene collinearity was observed only within certain clusters. Analysis of the dynamics of gene gain and loss along the phylogenetic tree of the NPVs showed that group 11 had only five defining genes and supported the hypothesis that these viruses form ten highly divergent ancient lineages. Crucially, more than 60 % of the gene gain events followed a power-Iaw relation to genetic distance among baculoviruses, indicative of temporal organization in the gene accretion processo.
650 $aPraga de planta
700 1 $aVALICENTE, F. H.
700 1 $aMARTINS, R.
700 1 $aOLIVEIRA, J. V. de C.
700 1 $aZANOTTO, P. M. de A.
773 $tJournal of General Virology, Cambridge$gv. 89, n. 6, p. 1202-1211, 2008.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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Biblioteca |
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Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
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Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
01/03/2001 |
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Data da última atualização: |
25/05/2023 |
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Autoria: |
MELO, F. R.; FRANCO, O. L.; SILVA, M. C. M. da; SA, M. F. G. |
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Afiliação: |
Embrapa Recursos Geneticos e Biotecnologia. |
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Título: |
Inibidores protéicos de plantas e suas atividades contra insetos. |
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Ano de publicação: |
1999 |
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Fonte/Imprenta: |
Brasilia: Embrapa Recursos Geneticos e Biotecnologia, 1999 |
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Páginas: |
50p. |
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Série: |
(Embrapa Recursos Geneticos e Biotecnologia. Circular Tecnica, 2) |
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Idioma: |
Português |
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Conteúdo: |
As pragas e os patogenos (fungos, bacterias e virus) sao responsaveis por grandes perdas da agricultura, causando injurias e doencas, alem de se alimentarem dos tecidos de plantas. As perdas na producao da agricultura mundial, devido ao ataque de pragas e doencas chegam a 37%, sendo que 13% desta perda e causada por insetos (Gatehouse et al., 1992). As plantas, possuem entretanto, um certo grau de resistencia a insetos e ha muitos anos, tem-se estudado a biossintese e a regulacao de compostos quimicos de plantas associadas com essas defesas. Atualmente, sabe-se que esses defensivos sao encontrados em varios tecidos vegetais e entre esses compostos, estao incluidos antibioticos, alcaloides, terpenos e proteinas. Entre as proteinas, estao incluidas enzimas tais como as quitinases, as lectinas e os inibidores de enzimas digestivas (Ryan, 1990). Atualmente, genes que conferem resistencia a insetos podem ser introduzidos em plantas de interesse para reduzir sua susceptibilidade. Esses genes podem ser obtidos de plantas, bacterias ou de outra origem (Schuler et al., 1998). Os inibidores de enzimas (a- amilases e e de proteinases) serao aqui descritos e estudados, relacionando-se suas funcoes como compostos de defesas de plantas contra insetos e seu potencial como ferramenta na obtencao de plantas resistentes a pragas. |
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Palavras-Chave: |
Controle de praga; Effectors; Ensima; Enzyme inbitors; Genetic; Inbidor de enzima; Inhibitors; Inibidor genético; Insect; Perdas da producao; pest; Pests of plants; Plant; Plant protein; Plants; Protein inhibitor; Resistance; Resistência a peste. |
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Thesagro: |
Amilase; Controle Biológico; Enzima; Genética; Inibidor de Proteína; Inseto; Patógeno; Planta; Praga; Praga de Planta; Proteína; Resistência; Variedade Resistente. |
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Thesaurus NAL: |
amylases; crop losses; disease resistance; enzymes; Insecta; pathogens; pest control; pest resistance; proteins. |
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Categoria do assunto: |
--
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://www.infoteca.cnptia.embrapa.br/infoteca/bitstream/doc/179931/1/52520001.pdf
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Marc: |
LEADER 02987nam a2200649 a 4500
001 1179931
005 2023-05-25
008 1999 bl uuuu 00u1 u #d
100 1 $aMELO, F. R.
245 $aInibidores protéicos de plantas e suas atividades contra insetos.
260 $aBrasilia: Embrapa Recursos Geneticos e Biotecnologia$c1999
300 $a50p.
490 $a(Embrapa Recursos Geneticos e Biotecnologia. Circular Tecnica, 2)
520 $aAs pragas e os patogenos (fungos, bacterias e virus) sao responsaveis por grandes perdas da agricultura, causando injurias e doencas, alem de se alimentarem dos tecidos de plantas. As perdas na producao da agricultura mundial, devido ao ataque de pragas e doencas chegam a 37%, sendo que 13% desta perda e causada por insetos (Gatehouse et al., 1992). As plantas, possuem entretanto, um certo grau de resistencia a insetos e ha muitos anos, tem-se estudado a biossintese e a regulacao de compostos quimicos de plantas associadas com essas defesas. Atualmente, sabe-se que esses defensivos sao encontrados em varios tecidos vegetais e entre esses compostos, estao incluidos antibioticos, alcaloides, terpenos e proteinas. Entre as proteinas, estao incluidas enzimas tais como as quitinases, as lectinas e os inibidores de enzimas digestivas (Ryan, 1990). Atualmente, genes que conferem resistencia a insetos podem ser introduzidos em plantas de interesse para reduzir sua susceptibilidade. Esses genes podem ser obtidos de plantas, bacterias ou de outra origem (Schuler et al., 1998). Os inibidores de enzimas (a- amilases e e de proteinases) serao aqui descritos e estudados, relacionando-se suas funcoes como compostos de defesas de plantas contra insetos e seu potencial como ferramenta na obtencao de plantas resistentes a pragas.
650 $aamylases
650 $acrop losses
650 $adisease resistance
650 $aenzymes
650 $aInsecta
650 $apathogens
650 $apest control
650 $apest resistance
650 $aproteins
650 $aAmilase
650 $aControle Biológico
650 $aEnzima
650 $aGenética
650 $aInibidor de Proteína
650 $aInseto
650 $aPatógeno
650 $aPlanta
650 $aPraga
650 $aPraga de Planta
650 $aProteína
650 $aResistência
650 $aVariedade Resistente
653 $aControle de praga
653 $aEffectors
653 $aEnsima
653 $aEnzyme inbitors
653 $aGenetic
653 $aInbidor de enzima
653 $aInhibitors
653 $aInibidor genético
653 $aInsect
653 $aPerdas da producao
653 $apest
653 $aPests of plants
653 $aPlant
653 $aPlant protein
653 $aPlants
653 $aProtein inhibitor
653 $aResistance
653 $aResistência a peste
700 1 $aFRANCO, O. L.
700 1 $aSILVA, M. C. M. da
700 1 $aSA, M. F. G.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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