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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
29/08/2002 |
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Data da última atualização: |
07/06/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
LOGUERCIO, L. L.; BARRETO, M. L.; ROCHA, T. L.; SANTOS, C. G.; TEIXEIRA, F. F.; PAIVA, E. |
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Afiliação: |
FLAVIA FRANCA TEIXEIRA, CNPMS. |
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Título: |
Combined analysis of supernatant-based feeding bioassay and PCR as a first-tier screening strategy for Vip-derived activities in Bacillus thuringiensis strains effective against tropical fall armyworm. |
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Ano de publicação: |
2002 |
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Fonte/Imprenta: |
Journal of Applied Microbiology, Danvers, v. 93, n. 2, p. 269-277, 2002. |
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Idioma: |
Inglês |
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Conteúdo: |
Aims: To assess whether feeding bioassays using culture-supernatant proteins could be combined with PCR into a first-tier screening strategy for Vip3A-like genes efficient against tropical Spodoptera frugiperda. Methods and Results: Out of 12 Bacillus thuringiensis strains studied, the total protein concentrated from the culture supernatant of only the strain HD125 yielded a significantly increased armyworm mortality and an intense band of the predicted size for VIP3A protein in SDS-PAGE. However, PCR and sequencing data indicated Vip-like genes are ubiquitous in tropical B. thuringiensis isolated. Interestingly, the HD125 strain was also the only one displaying a single-band amplification pattern and the highest sequence identity to the reported Vip3A(a) gene. Conclusions: Results suggest the insecticidal effectiveness of putative VIPs in B. thuringiensis isolates can be preliminarily estimated by the use of supernatant-derived total protein in feeding experiments, though only in a limited manner. Significance and impact of the study: a simple and cost-effective first-tier screening strategy for VIP-derived activities in B. thuringiensis collections can be developed by combining PCR and feeding bioassays. Moreover, the employed primers showed to be useful as a tool forr strains differentiation at DNA level, and for characterization and isolation of Vip-like genes in tropical B. thuringiensis germplasm. |
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Thesagro: |
Genética. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02123naa a2200193 a 4500
001 1485545
005 2018-06-07
008 2002 bl uuuu u00u1 u #d
100 1 $aLOGUERCIO, L. L.
245 $aCombined analysis of supernatant-based feeding bioassay and PCR as a first-tier screening strategy for Vip-derived activities in Bacillus thuringiensis strains effective against tropical fall armyworm.$h[electronic resource]
260 $c2002
520 $aAims: To assess whether feeding bioassays using culture-supernatant proteins could be combined with PCR into a first-tier screening strategy for Vip3A-like genes efficient against tropical Spodoptera frugiperda. Methods and Results: Out of 12 Bacillus thuringiensis strains studied, the total protein concentrated from the culture supernatant of only the strain HD125 yielded a significantly increased armyworm mortality and an intense band of the predicted size for VIP3A protein in SDS-PAGE. However, PCR and sequencing data indicated Vip-like genes are ubiquitous in tropical B. thuringiensis isolated. Interestingly, the HD125 strain was also the only one displaying a single-band amplification pattern and the highest sequence identity to the reported Vip3A(a) gene. Conclusions: Results suggest the insecticidal effectiveness of putative VIPs in B. thuringiensis isolates can be preliminarily estimated by the use of supernatant-derived total protein in feeding experiments, though only in a limited manner. Significance and impact of the study: a simple and cost-effective first-tier screening strategy for VIP-derived activities in B. thuringiensis collections can be developed by combining PCR and feeding bioassays. Moreover, the employed primers showed to be useful as a tool forr strains differentiation at DNA level, and for characterization and isolation of Vip-like genes in tropical B. thuringiensis germplasm.
650 $aGenética
700 1 $aBARRETO, M. L.
700 1 $aROCHA, T. L.
700 1 $aSANTOS, C. G.
700 1 $aTEIXEIRA, F. F.
700 1 $aPAIVA, E.
773 $tJournal of Applied Microbiology, Danvers$gv. 93, n. 2, p. 269-277, 2002.
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Embrapa Milho e Sorgo (CNPMS) |
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Registro Completo
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
28/01/2011 |
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Data da última atualização: |
30/04/2013 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
GUADAGNIN, J. P.; CARGNELUTTI FILHO, A.; EMYGDIO, B. M.; AMES, C. G.; BUZZETTI, D.; POSSA, F.; SANTOS, F. M. dos; MACHADO, J.; BEGNINI, J. C.; WINKLER, L.; GARRAFA, M.; ROMAN, P.; CARBONERA, R.; TRENTIN, R.; CASTRO, R. L. de; RODRIGUES, L. R. |
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Afiliação: |
JOSÉ PAULO GUADAGNIN, FEPAGRO; ALBERTO CARGNELUTTI FILHO, UFSM; BEATRIZ MARTI EMYGDIO, CPACT; CLAUDEMIR G. AMES, EMATER; DEJAMO BUZZETTI, EMATER; FELIPE POSSA, SEMILHA; FERNANDO M. DOS SANTOS, SETREM; JANE RODRIGUES DE ASSIS MACHADO, CNPMS; JOÃO CARLOS BEGNINI, FEPAGRO; LARISSA WINKLER, FUNDACEP; MARCOS GARRAFA, IFRS; PAULO ROMAN, COSUEL; ROBERTO CARBONERA, UNIJUÍ; RENATO TRENTIN, Monsanto; RICARDO LIMA DE CASTRO, FEPAGRO; LIA ROSANE RODRIGUES, FEPAGRO. |
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Título: |
Avaliação de cultivares de milho de ciclo precoce para indicação no Estado do Rio Grande do Sul na safra 2009-2010. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
In: REUNIÃO TÉCNICA ANUAL DO MILHO, 55.; REUNIÃO TÉCNICA ANUAL DO SORGO, 38., 2010, Vacaria. Atas e resumos. Vacaria: FEPAGRO: ASAV: Emater, 2010. p. 66-72. |
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Idioma: |
Português |
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Thesagro: |
Variedade; Zea mays. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/82202/1/Avaliacao-cultivares-5-1.pdf
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Marc: |
LEADER 00999nam a2200301 a 4500
001 1874974
005 2013-04-30
008 2010 bl uuuu u00u1 u #d
100 1 $aGUADAGNIN, J. P.
245 $aAvaliação de cultivares de milho de ciclo precoce para indicação no Estado do Rio Grande do Sul na safra 2009-2010.$h[electronic resource]
260 $aIn: REUNIÃO TÉCNICA ANUAL DO MILHO, 55.; REUNIÃO TÉCNICA ANUAL DO SORGO, 38., 2010, Vacaria. Atas e resumos. Vacaria: FEPAGRO: ASAV: Emater, 2010. p. 66-72.$c2010
650 $aVariedade
650 $aZea mays
700 1 $aCARGNELUTTI FILHO, A.
700 1 $aEMYGDIO, B. M.
700 1 $aAMES, C. G.
700 1 $aBUZZETTI, D.
700 1 $aPOSSA, F.
700 1 $aSANTOS, F. M. dos
700 1 $aMACHADO, J.
700 1 $aBEGNINI, J. C.
700 1 $aWINKLER, L.
700 1 $aGARRAFA, M.
700 1 $aROMAN, P.
700 1 $aCARBONERA, R.
700 1 $aTRENTIN, R.
700 1 $aCASTRO, R. L. de
700 1 $aRODRIGUES, L. R.
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