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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
07/01/1998 |
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Data da última atualização: |
13/07/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
LOPES, M. A.; LARKINS, B. A. |
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Afiliação: |
EMBRAPA-CNPMS. |
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Título: |
Gamma-zein content is related to endosperm modification in quality protein maize. |
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Ano de publicação: |
1991 |
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Fonte/Imprenta: |
Crop Science, Madison, v. 31, n. 6, p. 1655-1662, 1991. |
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Idioma: |
Inglês |
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Conteúdo: |
Opaque-2 (o2) is a mutation in one of the regulatory loci that control storage protein gene transcription in maize (Zea mays L.) kernels. It results in a significant reduction in the prolamine content of the endosperm, which becomes chalky and soft. Quality protein Maize (QPM) genotypes are modified, hard endosperm, o2 mutants that carry genetic factors responsible for increased synthesis of the y-zein protein (mol. wt. 27 kD). The genes encoding this storage protein are not under regulatory control of the 02 locus. We used a combination of genetic and biochemical analysis to investigate the relationships between endosperm modification and y-zein content in QPM. The use of unmodified and modified genotypes allowed us to demonstrate, by reciprocal crosses and F2 segregation progenies, that degree of vitreousness and increased synthesis of y-zein in modified endosperm are both dosage dependent and directly correlated. Additionally, we found that these same genetic factors are active in other soft-endosperm mutants such as floury-2 (fl2), and floury-2; opaque-2 (fl2;02), as well as in normal inbred lines. Although endosperm modication appears to be the result of a genetically complex system, its primary effect appears to be on the synthesis of the y-zein protein. Our studies suggest that there may be a relationship between the high content of thies protein and increase kernel vitreousness in QPM. |
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Palavras-Chave: |
Maize; Opaco-2; Opaque-2; Protein; QPM; Quality. |
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Thesagro: |
Biologia Molecular; Milho; Proteína; Qualidade; Zea Mays. |
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Thesaurus Nal: |
molecular biology. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02136naa a2200277 a 4500
001 1478284
005 2018-07-13
008 1991 bl uuuu u00u1 u #d
100 1 $aLOPES, M. A.
245 $aGamma-zein content is related to endosperm modification in quality protein maize.$h[electronic resource]
260 $c1991
520 $aOpaque-2 (o2) is a mutation in one of the regulatory loci that control storage protein gene transcription in maize (Zea mays L.) kernels. It results in a significant reduction in the prolamine content of the endosperm, which becomes chalky and soft. Quality protein Maize (QPM) genotypes are modified, hard endosperm, o2 mutants that carry genetic factors responsible for increased synthesis of the y-zein protein (mol. wt. 27 kD). The genes encoding this storage protein are not under regulatory control of the 02 locus. We used a combination of genetic and biochemical analysis to investigate the relationships between endosperm modification and y-zein content in QPM. The use of unmodified and modified genotypes allowed us to demonstrate, by reciprocal crosses and F2 segregation progenies, that degree of vitreousness and increased synthesis of y-zein in modified endosperm are both dosage dependent and directly correlated. Additionally, we found that these same genetic factors are active in other soft-endosperm mutants such as floury-2 (fl2), and floury-2; opaque-2 (fl2;02), as well as in normal inbred lines. Although endosperm modication appears to be the result of a genetically complex system, its primary effect appears to be on the synthesis of the y-zein protein. Our studies suggest that there may be a relationship between the high content of thies protein and increase kernel vitreousness in QPM.
650 $amolecular biology
650 $aBiologia Molecular
650 $aMilho
650 $aProteína
650 $aQualidade
650 $aZea Mays
653 $aMaize
653 $aOpaco-2
653 $aOpaque-2
653 $aProtein
653 $aQPM
653 $aQuality
700 1 $aLARKINS, B. A.
773 $tCrop Science, Madison$gv. 31, n. 6, p. 1655-1662, 1991.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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