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 | Acesso ao texto completo restrito à biblioteca da Embrapa Florestas. Para informações adicionais entre em contato com cnpf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
18/03/2009 |
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Data da última atualização: |
25/02/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SZANKOWSKI, I.; WAISMANN, S.; DEGENHARDT, J.; PATOCCHI, A.; PARIS, R.; SILFVERBERG-DILWORTH, E.; BROGGIANI, G.; GESSLER, C. |
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Afiliação: |
Iris Szankowski, Universidade de Hannover; Sascha Waidmann, Universidade de Hannover; Juliana Degenhardt, Embrapa Florestas; Andrea Patocchi, Agroscope Changins-Wadennswill; Roberta Paris, Universidade de Bologna; Eve Silfverberg-Dilworth, Institute of Integrative Biology/ETH Zurich; Giovanni Broggini, Institute of Integrative Biology/ETH Zurich; Cesare Gessler, Institute of Integrative Biology/ETH Zurich. |
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Título: |
Highly scab-resistant transgenic apple lines achieved by introgression of HcrVf2 controlled by different native promoter lengths. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Tree Genetics & Genomes, v. 5, p. 349-358, 2009. |
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DOI: |
10.1007/s11295-008-0191-8 |
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Idioma: |
Inglês |
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Conteúdo: |
Apple scab, caused by the ascomycete Venturia inaequalis, is the most damaging fungal disease of commercial apple orchards. Functional scab resistance genes are present in some wild Malus species. The HcrVf2 gene, derived from the Vf-region of the wild apple Malus floribunda 821 and encoding a receptor-like protein, has proved to confer scab resistance in a transgenic susceptible cultivar. In order to minimize nonplant DNA in genetically modified apple and to go a step toward the development of cisgenic apples, we have studied the capability of the HcrVf2 gene to confer apple scab resistance when it is controlled by its own promoter. Three promoter deletion constructs containing 115, 288, and 779 bp of the 5? untranslated region and the HcrVf2 gene were used to transform the scab susceptible apple cvs. ‘Gala’ and ‘Elstar.’ The influence of the promoter length on both the HcrVf2 expression level and the response to V. inaequalis was analyzed in different transgenic lines. Promoter length was found to influence both the constitutive transcription levels of HcrVf2 in transgenic lines and the resistance level. Highly scab resistant ‘Elstar’ and ‘Gala’ plants were obtained, proving that the HcrVf2 gene controlled by its native promoter is effective in conferring resistance to V. inaequalis similarly as Vf introgressed in apple cvs. through classical breeding. |
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Palavras-Chave: |
Malus venturia. |
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Thesagro: |
Maçã; Resistência. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02161naa a2200253 a 4500
001 1305512
005 2015-02-25
008 2009 bl uuuu u00u1 u #d
024 7 $a10.1007/s11295-008-0191-8$2DOI
100 1 $aSZANKOWSKI, I.
245 $aHighly scab-resistant transgenic apple lines achieved by introgression of HcrVf2 controlled by different native promoter lengths.$h[electronic resource]
260 $c2009
520 $aApple scab, caused by the ascomycete Venturia inaequalis, is the most damaging fungal disease of commercial apple orchards. Functional scab resistance genes are present in some wild Malus species. The HcrVf2 gene, derived from the Vf-region of the wild apple Malus floribunda 821 and encoding a receptor-like protein, has proved to confer scab resistance in a transgenic susceptible cultivar. In order to minimize nonplant DNA in genetically modified apple and to go a step toward the development of cisgenic apples, we have studied the capability of the HcrVf2 gene to confer apple scab resistance when it is controlled by its own promoter. Three promoter deletion constructs containing 115, 288, and 779 bp of the 5? untranslated region and the HcrVf2 gene were used to transform the scab susceptible apple cvs. ‘Gala’ and ‘Elstar.’ The influence of the promoter length on both the HcrVf2 expression level and the response to V. inaequalis was analyzed in different transgenic lines. Promoter length was found to influence both the constitutive transcription levels of HcrVf2 in transgenic lines and the resistance level. Highly scab resistant ‘Elstar’ and ‘Gala’ plants were obtained, proving that the HcrVf2 gene controlled by its native promoter is effective in conferring resistance to V. inaequalis similarly as Vf introgressed in apple cvs. through classical breeding.
650 $aMaçã
650 $aResistência
653 $aMalus venturia
700 1 $aWAISMANN, S.
700 1 $aDEGENHARDT, J.
700 1 $aPATOCCHI, A.
700 1 $aPARIS, R.
700 1 $aSILFVERBERG-DILWORTH, E.
700 1 $aBROGGIANI, G.
700 1 $aGESSLER, C.
773 $tTree Genetics & Genomes$gv. 5, p. 349-358, 2009.
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Registro original: |
Embrapa Florestas (CNPF) |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
20/01/2016 |
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Data da última atualização: |
18/11/2022 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
ROSA, P. M. da S.; CAMARGO, A. J. dos R.; SERAPIÃO, R. V.; CAMARGO, L. S. de A.; OLIVEIRA, C. S. |
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Afiliação: |
Paola Maria da Silva Rosa, USS; Agostinho Jorge dos Reis Camargo, PESAGRO RJ; Raquel Varella Serapião, PESAGRO RJ; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; CLARA SLADE OLIVEIRA, CNPGL. |
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Título: |
Eficiência reprodutiva de novilhas pré-púberes da raça Gir na produção in vitro de embriões. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
In: WORKSHOP DE INICIAÇÃO CIENTÍFICA DA EMBRAPA GADO DE LEITE, 16., 2015, Juiz de Fora, MG Anais... Juiz de Fora: Embrapa Gado de Leite, 2015. 1 CD-ROM. (Embrapa Gado de Leite. Documentos, 185.). |
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Páginas: |
2 p. |
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Idioma: |
Português |
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Palavras-Chave: |
Gir; Novilhas; Pré-púberes; Produção in vitro de embriões. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://www.alice.cnptia.embrapa.br/alice/bitstream/doc/1034560/1/Cnpgl2015WorkshopPibic12.pdf
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Marc: |
LEADER 00793nam a2200205 a 4500
001 2034560
005 2022-11-18
008 2015 bl uuuu u00u1 u #d
100 1 $aROSA, P. M. da S.
245 $aEficiência reprodutiva de novilhas pré-púberes da raça Gir na produção in vitro de embriões.$h[electronic resource]
260 $aIn: WORKSHOP DE INICIAÇÃO CIENTÍFICA DA EMBRAPA GADO DE LEITE, 16., 2015, Juiz de Fora, MG Anais... Juiz de Fora: Embrapa Gado de Leite, 2015. 1 CD-ROM. (Embrapa Gado de Leite. Documentos$c2015
300 $a2 p.
653 $aGir
653 $aNovilhas
653 $aPré-púberes
653 $aProdução in vitro de embriões
700 1 $aCAMARGO, A. J. dos R.
700 1 $aSERAPIÃO, R. V.
700 1 $aCAMARGO, L. S. de A.
700 1 $aOLIVEIRA, C. S.
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