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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
18/11/2025 |
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Data da última atualização: |
18/11/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MASCARIN, G. M.; SHRESTHA, S.; DUNLAP, C. A.; RAMIREZ, J. L.; COLEMAN, J. J. |
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Afiliação: |
GABRIEL MOURA MASCARIN, CNPMA; SOMRAJ SHRESTHA, AUBURN UNIVERSITY; CHRISTOPHER A. DUNLAP, UNITED STATES DEPARTMENT OF AGRICULTURE; JOSE LUIS RAMIREZ, UNITED STATES DEPARTMENT OF AGRICULTURE; JEFFREY J. COLEMAN, AUBURN UNIVERSITY. |
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Título: |
Enhanced virulence through genetic engineering of Beauveria bassiana blastospores by overexpression of a cuticle-degrading endochitinase. |
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Ano de publicação: |
2025 |
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Fonte/Imprenta: |
Journal of Invertebrate Pathology, v.213, n., p.108420, 2025. |
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ISSN: |
0022-2011 |
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DOI: |
https://doi.org/10.1016/j.jip.2025.108420 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Genetic engineering of entomopathogenic fungi combined with fast-acting infective blastospores grown by liquid fermentation offers an innovative approach to develop more efficient mycoinsecticides targeting a broad spectrum of arthropod pests, thus minimizing the over-reliance on synthetic chemical insecticides. In this research, Beauveria bassiana (Ascomycota: Cordycipitaceae) was genetically engineered by integrating multiple copies of an endogenously derived Chit1 gene, which encodes a constitutively expressed chitinase putatively involved in insect cuticle degradation, into its genome, with the aim at enhancing blastospore virulence. Genomic analysis of two highly virulent mutants overexpressing Chit1 identified 4 and 2 copies of this gene in their genomes, designated as OEBbChit1-3 and OEBbChit1-5, which were significantly upregulated by approximately ∼ 3- to 5-fold higher compared to the wild-type (WT) strain, thus confirming elevated gene expression and chitinase activity consistent with overexpression of the target gene. Compared with the WT, the median time to lethality (LT50) of the OEBbChit1 mutants was shortened to 75.7 %, and the median lethal concentration (LC50) decreased approximately 4.6-log10 fold when blastospores were applied to Galleria mellonella larvae via the natural cuticle infection route. Among the phenotypic traits examined, oxidative stress was more pronounced in the highly virulent mutants, which exhibited slightly reduced blastospore production in vitro than the WT strain. Moreover, infections by the highly virulent mutants increased the density of total hemocytes relative to the WT and mock control, thus triggering a rapid insect immune response. Collectively, our findings provide compelling evidence of an innovative strategy based on the genetic improvement of B. bassiana by overexpression of the native chitinase in fast-acting blastospores, thus leading to enhanced virulence with minimal detrimental phenotypical effects in fungal development. MenosAbstract: Genetic engineering of entomopathogenic fungi combined with fast-acting infective blastospores grown by liquid fermentation offers an innovative approach to develop more efficient mycoinsecticides targeting a broad spectrum of arthropod pests, thus minimizing the over-reliance on synthetic chemical insecticides. In this research, Beauveria bassiana (Ascomycota: Cordycipitaceae) was genetically engineered by integrating multiple copies of an endogenously derived Chit1 gene, which encodes a constitutively expressed chitinase putatively involved in insect cuticle degradation, into its genome, with the aim at enhancing blastospore virulence. Genomic analysis of two highly virulent mutants overexpressing Chit1 identified 4 and 2 copies of this gene in their genomes, designated as OEBbChit1-3 and OEBbChit1-5, which were significantly upregulated by approximately ∼ 3- to 5-fold higher compared to the wild-type (WT) strain, thus confirming elevated gene expression and chitinase activity consistent with overexpression of the target gene. Compared with the WT, the median time to lethality (LT50) of the OEBbChit1 mutants was shortened to 75.7 %, and the median lethal concentration (LC50) decreased approximately 4.6-log10 fold when blastospores were applied to Galleria mellonella larvae via the natural cuticle infection route. Among the phenotypic traits examined, oxidative stress was more pronounced in the highly virulent mutants, which exhibited slightly reduced blastospore ... Mostrar Tudo |
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Palavras-Chave: |
Quitinase. |
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Thesagro: |
Beauveria Bassiana; Controle Biológico; Engenharia Genética; Fungo Para Controle Biológico; Recombinação. |
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Thesaurus Nal: |
Biological control agents; Blastospores; Chitinase; Entomopathogenic fungi; Genetic recombination; Virulence. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 03091naa a2200337 a 4500
001 2181609
005 2025-11-18
008 2025 bl uuuu u00u1 u #d
022 $a0022-2011
024 7 $ahttps://doi.org/10.1016/j.jip.2025.108420$2DOI
100 1 $aMASCARIN, G. M.
245 $aEnhanced virulence through genetic engineering of Beauveria bassiana blastospores by overexpression of a cuticle-degrading endochitinase.$h[electronic resource]
260 $c2025
520 $aAbstract: Genetic engineering of entomopathogenic fungi combined with fast-acting infective blastospores grown by liquid fermentation offers an innovative approach to develop more efficient mycoinsecticides targeting a broad spectrum of arthropod pests, thus minimizing the over-reliance on synthetic chemical insecticides. In this research, Beauveria bassiana (Ascomycota: Cordycipitaceae) was genetically engineered by integrating multiple copies of an endogenously derived Chit1 gene, which encodes a constitutively expressed chitinase putatively involved in insect cuticle degradation, into its genome, with the aim at enhancing blastospore virulence. Genomic analysis of two highly virulent mutants overexpressing Chit1 identified 4 and 2 copies of this gene in their genomes, designated as OEBbChit1-3 and OEBbChit1-5, which were significantly upregulated by approximately ∼ 3- to 5-fold higher compared to the wild-type (WT) strain, thus confirming elevated gene expression and chitinase activity consistent with overexpression of the target gene. Compared with the WT, the median time to lethality (LT50) of the OEBbChit1 mutants was shortened to 75.7 %, and the median lethal concentration (LC50) decreased approximately 4.6-log10 fold when blastospores were applied to Galleria mellonella larvae via the natural cuticle infection route. Among the phenotypic traits examined, oxidative stress was more pronounced in the highly virulent mutants, which exhibited slightly reduced blastospore production in vitro than the WT strain. Moreover, infections by the highly virulent mutants increased the density of total hemocytes relative to the WT and mock control, thus triggering a rapid insect immune response. Collectively, our findings provide compelling evidence of an innovative strategy based on the genetic improvement of B. bassiana by overexpression of the native chitinase in fast-acting blastospores, thus leading to enhanced virulence with minimal detrimental phenotypical effects in fungal development.
650 $aBiological control agents
650 $aBlastospores
650 $aChitinase
650 $aEntomopathogenic fungi
650 $aGenetic recombination
650 $aVirulence
650 $aBeauveria Bassiana
650 $aControle Biológico
650 $aEngenharia Genética
650 $aFungo Para Controle Biológico
650 $aRecombinação
653 $aQuitinase
700 1 $aSHRESTHA, S.
700 1 $aDUNLAP, C. A.
700 1 $aRAMIREZ, J. L.
700 1 $aCOLEMAN, J. J.
773 $tJournal of Invertebrate Pathology$gv.213, n., p.108420, 2025.
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