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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
27/05/2025 |
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Data da última atualização: |
27/05/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SANTOS, J. D. R.; SILVA, I. M. da; RAMOS, L. F. C.; NOGUEIRA, F. C. S.; CONTE JUNIOR, C. A.; BATISTA, R. I. T. P.; SOUZA-FABJAN, J. M. G. de; BARBOSA, N. O.; FONSECA, J. F. da; BRANDÃO, F. Z. |
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Afiliação: |
JULIANA DANTAS RODRIGUES SANTOS, UNIVERSIDADE FEDERAL FLUMINENSE; YARA MARTINS DA SILVA, UNIVERSIDADE FEDERAL DO RIO DE JANEIRO; LUÍS FELIPE COSTA RAMOS, UNIVERSIDADE FEDERAL DO RIO DE JANEIRO; FÁBIO CÉSAR SOUSA NOGUEIRA, UNIVERSIDADE FEDERAL DO RIO DE JANEIRO; CARLOS ADAM CONTE JUNIOR, UNIVERSIDADE FEDERAL DO RIO DE JANEIRO; RIBRIO IVAN TAVARES PEREIRA BATISTA, UNIVERSIDADE FEDERAL FLUMINENSE; JOANNA MARIA GONÇALVES DE SOUZA-FABJAN, UNIVERSIDADE FEDERAL FLUMINENSE; NATHÁLIA OLIVEIRA BARBOSA, UNIVERSIDADE FEDERAL FLUMINENSE; JEFERSON FERREIRA DA FONSECA, CNPC; FELIPE ZANDONADI BRANDÃO, UNIVERSIDADE FEDERAL FLUMINENSE. |
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Título: |
Hormonal protocol for cervical dilation modifies the protein profile of uterine lavage recovered from superovulated ewes. |
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Ano de publicação: |
2025 |
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Fonte/Imprenta: |
Research in Veterinary Science, v. 192, 1057062025, Aug. 2025. |
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DOI: |
https://doi.org/10.1016/j.rvsc.2025.105706 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Hormonal protocols for cervical dilation (HPCD) are essential for non-surgical embryo recovery in ewes, due to the cervical complexity of this species. However, the hormones used for this purpose can alter the uterine protein profile and impact the embryonic quality. To test this hypothesis, Santa Inês ewes were superovulated and subjected or not to the HPCD before uterine lavage and embryos recovery by laparotomy. The protein profile of the uterine lavage was determined by mass spectrometry. Overall, 1447 proteins were identified, of which 436 and 126 were found exclusively in the treated and control groups, respectively. A total of 17 proteins were highly expressed in the treated group (P < 0.05), such as glutathione transferase (related to prostaglandin biosynthesis) and osteopontin (involved in the regulation of estradiol). In the control group, five proteins were up-regulated (P < 0.05), including cofilin (linked to up-regulation of embryonic development). Based on the gene ontology, in both groups, the proteins most abundant in the uterine fluid are related to cellular and metabolic processes, in addition to locomotion and localization in the control group. In conclusion, the hormonal protocol for cervical dilation modifies the protein profile of uterine lavage recovered from superovulated ewes after embryo collection. Highlights: The protein profile of the uterine fluid of ewes was determined. Proteins most abundantly are related to prostaglandin biosynthesis. Proteins most abundantly are related to cellular and metabolic processes. The hormonal protocol for cervical dilation modifies the uterine protein profile. MenosAbstract: Hormonal protocols for cervical dilation (HPCD) are essential for non-surgical embryo recovery in ewes, due to the cervical complexity of this species. However, the hormones used for this purpose can alter the uterine protein profile and impact the embryonic quality. To test this hypothesis, Santa Inês ewes were superovulated and subjected or not to the HPCD before uterine lavage and embryos recovery by laparotomy. The protein profile of the uterine lavage was determined by mass spectrometry. Overall, 1447 proteins were identified, of which 436 and 126 were found exclusively in the treated and control groups, respectively. A total of 17 proteins were highly expressed in the treated group (P < 0.05), such as glutathione transferase (related to prostaglandin biosynthesis) and osteopontin (involved in the regulation of estradiol). In the control group, five proteins were up-regulated (P < 0.05), including cofilin (linked to up-regulation of embryonic development). Based on the gene ontology, in both groups, the proteins most abundant in the uterine fluid are related to cellular and metabolic processes, in addition to locomotion and localization in the control group. In conclusion, the hormonal protocol for cervical dilation modifies the protein profile of uterine lavage recovered from superovulated ewes after embryo collection. Highlights: The protein profile of the uterine fluid of ewes was determined. Proteins most abundantly are related to prostaglandin biosynthesi... Mostrar Tudo |
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Palavras-Chave: |
Embryo collection; MOET; Protocolo hormonal; Tratamentos hormonais. |
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Thesagro: |
Biossíntese; Ovelha; Ovino; Prostaglandina; Reprodução; Superovulação. |
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Thesaurus Nal: |
Biosynthesis; Prostaglandins; Proteomics; Reproduction; Sheep. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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| Registros recuperados : 4 | |
| 1. |  | CANGAHUALA-INOCENTE, G. C.; STEINER, N.; MALDONADO, S. B.; GUERRA, M. P. Patterns of protein and carbohydrate accumulation during somatic embryogenesis of Acca sellowiana. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 44, n. 3, p. 217-224, mar. 2009. Título em português: Padrões de acúmulo de proteínas e carboidratos durante a embriogênese somática de Acca sellowiana.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 3. | | CANGAHUALA-INOCENTE, G. C.; DAL VESCO, L. L.; STEINMACHER, D.; TORRES, A. C.; GUERRA, M. P. Improvements in somatic embryogenesis protocol in Feijoa (Acca sellowiana (Berg) Burret): induction, conversion and synthetic seeds. Scientia Horticulturae, Amsterdam, v. 111, p. 228-234, 2007.| Biblioteca(s): Embrapa Hortaliças. |
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| 4. | | CANGAHUALA-INOCENTE, G. C.; SILVA, M. F. da; JOHNSON, J.-M.; MANGA, A.; VAN TUINEN, D.; HENRY, C.; LOVATO, P. E.; DUMAS-GAUDOT, E. Arbuscular mycorrhizal symbiosis elicits proteome responses opposite of P-starvation in SO4 grapevine rootstock upon root colonisation with two Glomus species. Mycorrhiza, Berlin, v. 21, n. 6, p. 471-493, Jan. 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Amapá. |
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| Registros recuperados : 4 | |
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