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Registro Completo |
Biblioteca(s): |
Embrapa Instrumentação; Embrapa Milho e Sorgo. |
Data corrente: |
06/02/2025 |
Data da última atualização: |
06/02/2025 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
LODI, L. A.; LOPES, M. M.; GRACIANO, V. A.; BORGES, R.; OLIVEIRA-PAIVA, C. A.; OLIVEIRA, C. R. de; FARINAS, C. S. |
Afiliação: |
LUDIMILA ARAÚJO LODI, UNIVERSIDDE FEDERAL DE SAO CARLOS; MARINA MOMESSO LOPES, UNIVERSIDADE FEDERAL DE SAO CARLOS; VANESSA ARAUJO GRACIANO, UNIVERSIDADE FEDERAL DE SAO CARLOS; ROGER BORGES; CHRISTIANE ABREU DE OLIVEIRA PAIVA, CNPMS; CAUE RIBEIRO DE OLIVEIRA, CNPDIA; CRISTIANE SANCHEZ FARINAS, CNPDIA. |
Título: |
Microencapsulation of Bacillus megaterium in cationic starch/ PVA-based matrices. |
Ano de publicação: |
2025 |
Fonte/Imprenta: |
International Journal of Biological Macromolecules, v. 303, 140588, 2025. |
DOI: |
https://doi.org/10.1016/j.ijbiomac.2025.140588 |
Idioma: |
Inglês |
Conteúdo: |
The increasing demand for sustainable and effective agricultural bio-based products is driving innovation in encapsulation technologies for beneficial microorganisms, such as Bacillus strains, known for promoting plant growth and controlling pathogens. This study proposes an efficient methodology for encapsulating Bacillus megaterium within microparticles using a simple cross-linking/emulsion process based on Cationic Starch (CS) and Polyvinyl Alcohol (PVA). Citric acid (CA) and Sodium Trimetaphosphate (STMP) were employed as crosslinking agents, while bentonite (Bent) was added to modify the materials. The resulting microparticles exhibi ted a range of properties influenced by the cross-linking agents and bentonite. Encapsulation matrices with STMP and STMP-Bent demonstrated superior performance in providing controlled bacterial release and enhanced protection under adverse conditions. These matrices maintained up to 95 % of the initial cell viability after exposure to heat (55 ◦C for 24 h), and up to 98 % viability after exposure to UV light (180 min) or pesticides (30 ◦C for 24 h). In contrast, matrices containing CA or CA-Bent, as well as free bacteria, showed significantly lower bacterial survival rates in the same tests. In a high-salinity soil test (200 mM), STMP-based matrices provided the best protection for encapsulated bacteria, leading to a 10 % increase in B. megaterium growth. Furthermore, these matrices demonstrated an estimated shelf-life of over 40 months when stored at temperatures of 15 ◦C, 30 ◦C, and 45 ◦C. These findings suggest that CS/PVA matrices cross-linked with STMP offer a promising approach for developing more resilient and effective bio-based agricultural products. MenosThe increasing demand for sustainable and effective agricultural bio-based products is driving innovation in encapsulation technologies for beneficial microorganisms, such as Bacillus strains, known for promoting plant growth and controlling pathogens. This study proposes an efficient methodology for encapsulating Bacillus megaterium within microparticles using a simple cross-linking/emulsion process based on Cationic Starch (CS) and Polyvinyl Alcohol (PVA). Citric acid (CA) and Sodium Trimetaphosphate (STMP) were employed as crosslinking agents, while bentonite (Bent) was added to modify the materials. The resulting microparticles exhibi ted a range of properties influenced by the cross-linking agents and bentonite. Encapsulation matrices with STMP and STMP-Bent demonstrated superior performance in providing controlled bacterial release and enhanced protection under adverse conditions. These matrices maintained up to 95 % of the initial cell viability after exposure to heat (55 ◦C for 24 h), and up to 98 % viability after exposure to UV light (180 min) or pesticides (30 ◦C for 24 h). In contrast, matrices containing CA or CA-Bent, as well as free bacteria, showed significantly lower bacterial survival rates in the same tests. In a high-salinity soil test (200 mM), STMP-based matrices provided the best protection for encapsulated bacteria, leading to a 10 % increase in B. megaterium growth. Furthermore, these matrices demonstrated an estimated shelf-life of over 40 months wh... Mostrar Tudo |
Palavras-Chave: |
Controlled release; Emulsão Encapsulamento; Microbial inoculant; Tolerância ao estresse. |
Thesagro: |
Emulsão; Inoculante. |
Thesaurus Nal: |
Emulsions; Encapsulation; Stress tolerance. |
Categoria do assunto: |
S Ciências Biológicas |
Marc: |
LEADER 02684naa a2200313 a 4500 001 2172414 005 2025-02-06 008 2025 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/j.ijbiomac.2025.140588$2DOI 100 1 $aLODI, L. A. 245 $aMicroencapsulation of Bacillus megaterium in cationic starch/ PVA-based matrices.$h[electronic resource] 260 $c2025 520 $aThe increasing demand for sustainable and effective agricultural bio-based products is driving innovation in encapsulation technologies for beneficial microorganisms, such as Bacillus strains, known for promoting plant growth and controlling pathogens. This study proposes an efficient methodology for encapsulating Bacillus megaterium within microparticles using a simple cross-linking/emulsion process based on Cationic Starch (CS) and Polyvinyl Alcohol (PVA). Citric acid (CA) and Sodium Trimetaphosphate (STMP) were employed as crosslinking agents, while bentonite (Bent) was added to modify the materials. The resulting microparticles exhibi ted a range of properties influenced by the cross-linking agents and bentonite. Encapsulation matrices with STMP and STMP-Bent demonstrated superior performance in providing controlled bacterial release and enhanced protection under adverse conditions. These matrices maintained up to 95 % of the initial cell viability after exposure to heat (55 ◦C for 24 h), and up to 98 % viability after exposure to UV light (180 min) or pesticides (30 ◦C for 24 h). In contrast, matrices containing CA or CA-Bent, as well as free bacteria, showed significantly lower bacterial survival rates in the same tests. In a high-salinity soil test (200 mM), STMP-based matrices provided the best protection for encapsulated bacteria, leading to a 10 % increase in B. megaterium growth. Furthermore, these matrices demonstrated an estimated shelf-life of over 40 months when stored at temperatures of 15 ◦C, 30 ◦C, and 45 ◦C. These findings suggest that CS/PVA matrices cross-linked with STMP offer a promising approach for developing more resilient and effective bio-based agricultural products. 650 $aEmulsions 650 $aEncapsulation 650 $aStress tolerance 650 $aEmulsão 650 $aInoculante 653 $aControlled release 653 $aEmulsão Encapsulamento 653 $aMicrobial inoculant 653 $aTolerância ao estresse 700 1 $aLOPES, M. M. 700 1 $aGRACIANO, V. A. 700 1 $aBORGES, R. 700 1 $aOLIVEIRA-PAIVA, C. A. 700 1 $aOLIVEIRA, C. R. de 700 1 $aFARINAS, C. S. 773 $tInternational Journal of Biological Macromolecules$gv. 303, 140588, 2025.
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pesca e Aquicultura. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Pesca e Aquicultura. |
Data corrente: |
23/06/2020 |
Data da última atualização: |
01/10/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 1 |
Autoria: |
LIMA, A. F.; ALVES, R. R.; TORATI, L. S. |
Afiliação: |
ADRIANA FERREIRA LIMA, CNPASA; ROSIANA RODRIGUES ALVES, CNPASA; LUCAS SIMON TORATI, CNPASA. |
Título: |
Efficiency of color pattern as a method for sex identification in Arapaima gigas (Schinz, 1822). |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Pan-American Journal of Aquatic Sciences, v. 15, n. 2, p. 87-92, 2020. |
ISSN: |
1809-9009 |
Idioma: |
Inglês |
Conteúdo: |
his study evaluated the utility of color pattern as a tool for sex identification inbroodstocks of Arapaima gigas. To do so, 110 individuals from three study sites had their sexdetermined with vitellogenin (VTG). Then, presence of red pigmentation was systematicallyevaluated scoring from the head (0) counting scales from the base of pectoral fin towards fishtail (1, 2, 3,...). Sex and study site were the variables that had significant effects on the zero-inflated negative binomial model (p<0.01) to assess color pattern. Discriminant functionanalysis (DFA) yielded 82 % of correct classification for females and 76 % for males. With suchmethod, both sexes could be identified with similar hit rates. Differences were seen in sexualcolor pattern comparing the different study sites. Therefore, application of the method should berestricted to site-specific broodstocks rather than be a general rule for all populations. Inconclusion, sex identification in Arapaima broodstocks through color pattern may be considereda cheap alternative for broodstock management but should be used in combination with otheravailable methods to yield optimal rates for sex identification (i.e. endoscopy, cannulationand/or vitellogenin). |
Palavras-Chave: |
Dimorfismo sexual; Identificação sexual. |
Thesagro: |
Identificação; Manejo; Peixe; Pigmentação; Pirarucu; Reprodutor. |
Thesaurus NAL: |
Arapaima gigas; Fish; Sexual dimorphism. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02039naa a2200289 a 4500 001 2123419 005 2020-10-01 008 2020 bl uuuu u00u1 u #d 022 $a1809-9009 100 1 $aLIMA, A. F. 245 $aEfficiency of color pattern as a method for sex identification in Arapaima gigas (Schinz, 1822).$h[electronic resource] 260 $c2020 520 $ahis study evaluated the utility of color pattern as a tool for sex identification inbroodstocks of Arapaima gigas. To do so, 110 individuals from three study sites had their sexdetermined with vitellogenin (VTG). Then, presence of red pigmentation was systematicallyevaluated scoring from the head (0) counting scales from the base of pectoral fin towards fishtail (1, 2, 3,...). Sex and study site were the variables that had significant effects on the zero-inflated negative binomial model (p<0.01) to assess color pattern. Discriminant functionanalysis (DFA) yielded 82 % of correct classification for females and 76 % for males. With suchmethod, both sexes could be identified with similar hit rates. Differences were seen in sexualcolor pattern comparing the different study sites. Therefore, application of the method should berestricted to site-specific broodstocks rather than be a general rule for all populations. Inconclusion, sex identification in Arapaima broodstocks through color pattern may be considereda cheap alternative for broodstock management but should be used in combination with otheravailable methods to yield optimal rates for sex identification (i.e. endoscopy, cannulationand/or vitellogenin). 650 $aArapaima gigas 650 $aFish 650 $aSexual dimorphism 650 $aIdentificação 650 $aManejo 650 $aPeixe 650 $aPigmentação 650 $aPirarucu 650 $aReprodutor 653 $aDimorfismo sexual 653 $aIdentificação sexual 700 1 $aALVES, R. R. 700 1 $aTORATI, L. S. 773 $tPan-American Journal of Aquatic Sciences$gv. 15, n. 2, p. 87-92, 2020.
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