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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
30/06/2021 |
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Data da última atualização: |
30/06/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SANTOS, C. M. dos; RIBEIRO, N. V. S.; SCHWAB, S.; BALDANI, J. I.; VIDAL, M. S. |
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Afiliação: |
CARLOS M. DOS SANTOS, Bolsista da Embrapa Agrobiologia; NÁTHALIA V. S. RIBEIRO, Bolsista da Embrapa Agrobiologia; STEFAN SCHWAB, CNPAB; JOSE IVO BALDANI, CNPAB; MARCIA SOARES VIDAL, CNPAB. |
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Título: |
The effect of inoculation of a diazotrophic bacterial consortium on the indigenous bacterial community structure of sugarcane apoplast fluid. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Current Microbiologym, Published online, 25 june 2021. |
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ISSN: |
1432-0991 |
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DOI: |
https://doi.org/10.1007/s00284-021-02571-0 |
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Idioma: |
Inglês |
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Conteúdo: |
The extracellular space in plants, termed the apoplast, has a pH and sugar content that enables bacterial growth and represents a possible niche for the establishment of endophytic bacteria. Previous studies have investigated the effects of diazotrophic bacteria inoculation in sugarcane varieties, but it has not yet been analyzed how the microbial community of apoplast fluid of sugarcane is affected. High-throughput next generation sequencing of the 16S rRNA gene was used throughout this study to determine the effect of inoculation with a diazotrophic bacteria consortium, previously isolated from sugarcane, on the native bacterial population of sugarcane variety RB867515 grown in the field. The analyses were carried out 450 days after inoculation. The results revealed the presence of 22 phyla, with predominance of Proteobacteria phylum. It was observed that the inoculated consortium changed the indigenous bacterial community structure of sugarcane apoplast fluid by decreasing diversity and evenness, interfering in the composition of rare species. Microbial community composition analysis revealed differences between treatments. The differential abundance test showed there were 43 amplicon sequence variants (ASVs) which were relatively more abundant in the inoculated treatment, with predominance of the Sphingomonas genus. The pre-dicted functions of the most abundant ASVs revealed the presence of genera related to plant growth promotion and protection against phytopathogens. Analysis to evaluate the occurrence of inoculated strains in the recovered data was not conclusive since the ASVs taxonomically close to the inoculated bacteria were observed in low abundance. The present study is the first report to elucidate the bacterial community in sugarcane apoplast fluid using a culture-independent approach. It demonstrated that the diazotrophic bacterial consortium interferes in the natural bacterial community in sugarcane variety RB867515 MenosThe extracellular space in plants, termed the apoplast, has a pH and sugar content that enables bacterial growth and represents a possible niche for the establishment of endophytic bacteria. Previous studies have investigated the effects of diazotrophic bacteria inoculation in sugarcane varieties, but it has not yet been analyzed how the microbial community of apoplast fluid of sugarcane is affected. High-throughput next generation sequencing of the 16S rRNA gene was used throughout this study to determine the effect of inoculation with a diazotrophic bacteria consortium, previously isolated from sugarcane, on the native bacterial population of sugarcane variety RB867515 grown in the field. The analyses were carried out 450 days after inoculation. The results revealed the presence of 22 phyla, with predominance of Proteobacteria phylum. It was observed that the inoculated consortium changed the indigenous bacterial community structure of sugarcane apoplast fluid by decreasing diversity and evenness, interfering in the composition of rare species. Microbial community composition analysis revealed differences between treatments. The differential abundance test showed there were 43 amplicon sequence variants (ASVs) which were relatively more abundant in the inoculated treatment, with predominance of the Sphingomonas genus. The pre-dicted functions of the most abundant ASVs revealed the presence of genera related to plant growth promotion and protection against phytopathogens. A... Mostrar Tudo |
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Palavras-Chave: |
Endophytic bacteria; Plant growth promotion bacteria. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02707naa a2200217 a 4500
001 2132685
005 2021-06-30
008 2021 bl uuuu u00u1 u #d
022 $a1432-0991
024 7 $ahttps://doi.org/10.1007/s00284-021-02571-0$2DOI
100 1 $aSANTOS, C. M. dos
245 $aThe effect of inoculation of a diazotrophic bacterial consortium on the indigenous bacterial community structure of sugarcane apoplast fluid.$h[electronic resource]
260 $c2021
520 $aThe extracellular space in plants, termed the apoplast, has a pH and sugar content that enables bacterial growth and represents a possible niche for the establishment of endophytic bacteria. Previous studies have investigated the effects of diazotrophic bacteria inoculation in sugarcane varieties, but it has not yet been analyzed how the microbial community of apoplast fluid of sugarcane is affected. High-throughput next generation sequencing of the 16S rRNA gene was used throughout this study to determine the effect of inoculation with a diazotrophic bacteria consortium, previously isolated from sugarcane, on the native bacterial population of sugarcane variety RB867515 grown in the field. The analyses were carried out 450 days after inoculation. The results revealed the presence of 22 phyla, with predominance of Proteobacteria phylum. It was observed that the inoculated consortium changed the indigenous bacterial community structure of sugarcane apoplast fluid by decreasing diversity and evenness, interfering in the composition of rare species. Microbial community composition analysis revealed differences between treatments. The differential abundance test showed there were 43 amplicon sequence variants (ASVs) which were relatively more abundant in the inoculated treatment, with predominance of the Sphingomonas genus. The pre-dicted functions of the most abundant ASVs revealed the presence of genera related to plant growth promotion and protection against phytopathogens. Analysis to evaluate the occurrence of inoculated strains in the recovered data was not conclusive since the ASVs taxonomically close to the inoculated bacteria were observed in low abundance. The present study is the first report to elucidate the bacterial community in sugarcane apoplast fluid using a culture-independent approach. It demonstrated that the diazotrophic bacterial consortium interferes in the natural bacterial community in sugarcane variety RB867515
653 $aEndophytic bacteria
653 $aPlant growth promotion bacteria
700 1 $aRIBEIRO, N. V. S.
700 1 $aSCHWAB, S.
700 1 $aBALDANI, J. I.
700 1 $aVIDAL, M. S.
773 $tCurrent Microbiologym, Published online, 25 june 2021.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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Biblioteca |
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Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Suínos e Aves; Embrapa Unidades Centrais. |
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Data corrente: |
19/12/2017 |
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Data da última atualização: |
21/02/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
PALADINO, E. S.; GABARDO, M. de P.; LUNARDI, P. N.; MORES, N.; GUEDES, R. M. C. |
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Afiliação: |
Eliana S. Paladino, Departamento de Clinica e Cirurgia Veterinária/Escola de Veterinária/Universidade Federal de Minas Gerais - UFMG; Michelle de P. Gabardo, Departamento de Clinica e Cirurgia Veterinária/Escola de Veterinária/Universidade Federal de Minas Gerais - UFMG; Priscilla N. Lunardi, Departamento de Clinica e Cirurgia Veterinária/Escola de Veterinária/Universidade Federal de Minas Gerais - UFMG; NELSON MORES, CNPSA; Roberto M. C. Guedes, Departamento de Clinica e Cirurgia Veterinária/Escola de Veterinária/Universidade Federal de Minas Gerais - UFMG. |
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Título: |
Anatomopathological pneumonic aspects associated with highly pathogenic Pasteurella multocida in finishing pigs. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 37, n, 10, p. 1091-1100, outubro 2017. |
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Idioma: |
Inglês |
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Notas: |
Título em português: Aspectos pneumônicos anatomo-patológicos associados a Pasteurella multocida de alta patogenicidade em suínos de terminação. |
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Conteúdo: |
The bacterium Pasteurella multocida is a frequent cause of porcine respiratory disease complex in finishing pigs. Historically, the bacterium is recognized as an opportunistic agent, causing secondary bacterial pneumonia in pigs. Several Brazilian reports have suggested the ability of P. multocida to cause primary pulmonary infection that leads to the death of finishing pigs prior to slaughter. The aim of this study was to evaluate anatomopathological pulmonary findings associated with P. multocida infection that were obtained from animals with clinical respiratory disease and from animals at slaughter. Twenty-five lung samples from 14 herds of finishing pigs with acute clinical respiratory disease and 19 lungs collected at slaughter from a different set of 14 herds were studied. In all lung samples, bacterial isolation was performed, and only samples with pure P. multocida growth were included in the study. Gross and histopathological lesions were evaluated, as well as Influenza A, porcine circovirus type 2 (PCV2) and Mycoplasma hyopneumoniae co-infections. Pleuritis and pericarditis were more often observed in clinical samples (P<0.05). Moreover, there was a numerical trend indicating that pericarditis, lymphadenomegaly and cavity exudates were more often present in clinical samples. Thirteen lung samples were negative to M. hyopneumoniae, Influenza A and PCV2 by immunohistochemistry (IHC), with only P. multocida identified. In these cases, gross lesions such as pleuritis, pericarditis and lymphadenomegaly were always present, and no histologic lesions indicative of other agents such as Actinobacillus pleuropneumoniae, Actinobacillus suis or Haemophilus parasuis were observed. These findings suggest the ability of some P. multocida isolates to cause primary respiratory and systemic infection. However, in this study, it was not possible to determine specific virulence markers to explain these findings. MenosThe bacterium Pasteurella multocida is a frequent cause of porcine respiratory disease complex in finishing pigs. Historically, the bacterium is recognized as an opportunistic agent, causing secondary bacterial pneumonia in pigs. Several Brazilian reports have suggested the ability of P. multocida to cause primary pulmonary infection that leads to the death of finishing pigs prior to slaughter. The aim of this study was to evaluate anatomopathological pulmonary findings associated with P. multocida infection that were obtained from animals with clinical respiratory disease and from animals at slaughter. Twenty-five lung samples from 14 herds of finishing pigs with acute clinical respiratory disease and 19 lungs collected at slaughter from a different set of 14 herds were studied. In all lung samples, bacterial isolation was performed, and only samples with pure P. multocida growth were included in the study. Gross and histopathological lesions were evaluated, as well as Influenza A, porcine circovirus type 2 (PCV2) and Mycoplasma hyopneumoniae co-infections. Pleuritis and pericarditis were more often observed in clinical samples (P<0.05). Moreover, there was a numerical trend indicating that pericarditis, lymphadenomegaly and cavity exudates were more often present in clinical samples. Thirteen lung samples were negative to M. hyopneumoniae, Influenza A and PCV2 by immunohistochemistry (IHC), with only P. multocida identified. In these cases, gross lesions such as pleuriti... Mostrar Tudo |
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Palavras-Chave: |
Fibrina. |
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Thesagro: |
Pasteurella Multocida; Pasteurelose; Pleuropneumonia; Pneumonia. |
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Thesaurus NAL: |
Fibrin; Pasteurellosis; Swine. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/169235/1/Anatomopathological-pneumonic-aspects.pdf
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Marc: |
LEADER 02910naa a2200277 a 4500
001 2088003
005 2018-02-21
008 2017 bl uuuu u00u1 u #d
100 1 $aPALADINO, E. S.
245 $aAnatomopathological pneumonic aspects associated with highly pathogenic Pasteurella multocida in finishing pigs.$h[electronic resource]
260 $c2017
500 $aTítulo em português: Aspectos pneumônicos anatomo-patológicos associados a Pasteurella multocida de alta patogenicidade em suínos de terminação.
520 $aThe bacterium Pasteurella multocida is a frequent cause of porcine respiratory disease complex in finishing pigs. Historically, the bacterium is recognized as an opportunistic agent, causing secondary bacterial pneumonia in pigs. Several Brazilian reports have suggested the ability of P. multocida to cause primary pulmonary infection that leads to the death of finishing pigs prior to slaughter. The aim of this study was to evaluate anatomopathological pulmonary findings associated with P. multocida infection that were obtained from animals with clinical respiratory disease and from animals at slaughter. Twenty-five lung samples from 14 herds of finishing pigs with acute clinical respiratory disease and 19 lungs collected at slaughter from a different set of 14 herds were studied. In all lung samples, bacterial isolation was performed, and only samples with pure P. multocida growth were included in the study. Gross and histopathological lesions were evaluated, as well as Influenza A, porcine circovirus type 2 (PCV2) and Mycoplasma hyopneumoniae co-infections. Pleuritis and pericarditis were more often observed in clinical samples (P<0.05). Moreover, there was a numerical trend indicating that pericarditis, lymphadenomegaly and cavity exudates were more often present in clinical samples. Thirteen lung samples were negative to M. hyopneumoniae, Influenza A and PCV2 by immunohistochemistry (IHC), with only P. multocida identified. In these cases, gross lesions such as pleuritis, pericarditis and lymphadenomegaly were always present, and no histologic lesions indicative of other agents such as Actinobacillus pleuropneumoniae, Actinobacillus suis or Haemophilus parasuis were observed. These findings suggest the ability of some P. multocida isolates to cause primary respiratory and systemic infection. However, in this study, it was not possible to determine specific virulence markers to explain these findings.
650 $aFibrin
650 $aPasteurellosis
650 $aSwine
650 $aPasteurella Multocida
650 $aPasteurelose
650 $aPleuropneumonia
650 $aPneumonia
653 $aFibrina
700 1 $aGABARDO, M. de P.
700 1 $aLUNARDI, P. N.
700 1 $aMORES, N.
700 1 $aGUEDES, R. M. C.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 37, n, 10, p. 1091-1100, outubro 2017.
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Embrapa Suínos e Aves (CNPSA) |
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