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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
15/01/2019 |
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Data da última atualização: |
15/01/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
LIMA, G. da S.; ROCHA, A. M. da; SANTOS, G. F. dos; SILVA, A. F.; MARRIEL, I. E.; TAKAHASHI, J. A. |
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Afiliação: |
Gesiane da Silva Lima, Universidade Federal de Minas Gerais; Aline Mendes da Rocha, Universidade Federal de Minas Gerais; Gabriel Franco dos Santos, Universidade Federal de Minas Gerais; Alice Ferreira Silva, Universidade Federal de Minas Gerais; IVANILDO EVODIO MARRIEL, CNPMS; Jacqueline Aparecida Takahashi, Universidade Federal de Minas Gerais. |
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Título: |
Metabolic response of Aspergillus sydowii to OSMAC modulation produces acetylcholinesterase inhibitors. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Phytochemistry Letters, v. 24, p. 39-45, 2018. |
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DOI: |
10.1016/j.phytol.2018.01.007 |
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Idioma: |
Inglês |
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Conteúdo: |
Fungi are an extraordinary source of bioactive metabolites especially because their metabolic profile can be modulated according to the conditions under which they are grown, a strategy known as OSMAC. Aspergillus sydowii has been showing to be a fungal species with a series of interesting biosynthetic pathways, but production of acethylcholinesterase inhibitors was still not sufficiently explored from this fungal species. In this work, A. sydowii was grown in three different media aiming at awaking biosynthetic routes silent in the works already reported in the literature, in order to produce acethylcholinesterase inhibitors. Using sucrose (150g/L) and peptone (12g/L) as sources of carbon and nitrogen respectively, a very active extract was obtained (84.5 + - 2.2% of acethylcholinesterase inhibition). Metabolites 1-5 were isolated from this extract. These metabolites are described from A. sydowii by the first time, to the best of our knowledge. Compound 2 was the most active AChE inhibitor (93.25 + - 0.41%). The results show how small changes in culturing conditions can modulate the production of bioactive secondary metabolites that are silent in other cultivation conditions. |
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Thesagro: |
Fungo. |
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Thesaurus Nal: |
Aspergillus; Diketopiperazines. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 01885naa a2200229 a 4500
001 2104256
005 2019-01-15
008 2018 bl uuuu u00u1 u #d
024 7 $a10.1016/j.phytol.2018.01.007$2DOI
100 1 $aLIMA, G. da S.
245 $aMetabolic response of Aspergillus sydowii to OSMAC modulation produces acetylcholinesterase inhibitors.$h[electronic resource]
260 $c2018
520 $aFungi are an extraordinary source of bioactive metabolites especially because their metabolic profile can be modulated according to the conditions under which they are grown, a strategy known as OSMAC. Aspergillus sydowii has been showing to be a fungal species with a series of interesting biosynthetic pathways, but production of acethylcholinesterase inhibitors was still not sufficiently explored from this fungal species. In this work, A. sydowii was grown in three different media aiming at awaking biosynthetic routes silent in the works already reported in the literature, in order to produce acethylcholinesterase inhibitors. Using sucrose (150g/L) and peptone (12g/L) as sources of carbon and nitrogen respectively, a very active extract was obtained (84.5 + - 2.2% of acethylcholinesterase inhibition). Metabolites 1-5 were isolated from this extract. These metabolites are described from A. sydowii by the first time, to the best of our knowledge. Compound 2 was the most active AChE inhibitor (93.25 + - 0.41%). The results show how small changes in culturing conditions can modulate the production of bioactive secondary metabolites that are silent in other cultivation conditions.
650 $aAspergillus
650 $aDiketopiperazines
650 $aFungo
700 1 $aROCHA, A. M. da
700 1 $aSANTOS, G. F. dos
700 1 $aSILVA, A. F.
700 1 $aMARRIEL, I. E.
700 1 $aTAKAHASHI, J. A.
773 $tPhytochemistry Letters$gv. 24, p. 39-45, 2018.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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