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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
15/02/2016 |
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Data da última atualização: |
26/09/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FANÉ, R.; TRAORÉ, D.; KASSOGUÉ, A.; SAMALÉ, F.; DICKO, A. H.; HAMADOUN, A.; VALICENTE, F. H.; BABANA, A. H. |
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Afiliação: |
FERNANDO HERCOS VALICENTE, CNPMS. |
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Título: |
Genetic diversity of native Bacillus thuringiensis strains isolated from soil of different localities in Mali and their cry gene profile. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
British Biotechnology Journal, v. 12, n. 1, p. 1-8, 2016. |
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DOI: |
10.9734/BBJ/2016/20432 |
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Idioma: |
Inglês |
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Conteúdo: |
Aims: To determine the genetic diversity of Bacillus thuringiensis strains isolated from soils of different locality in Mali, and select strains with cry1F, cry1B and cry1C genes to control caterpillars and strains with cry2 gene against African rice gall midge. Study Design: Strains of Bacillus thuringiensis (Bt) used in this study belong to collection of Laboratory of Research in Microbiology and Microbial Biotechnology (Laborem-Biotech) and that isolated from different ecological environment of Mali. Bt strains used as positive controls were kindly provided by Dr. Fernando Hercos Valicente from Embrapa Milho e Sorgo (Brazil). Methodology: The total DNA of the native B. thuringiensis and reference strains were extracted from overnight grown culture. Gene identification was performed by amplification (PCR) of DNA of Bt strains using specific primers. The gel revelation was performed using ethidium bromide and the gel photography was performed using an E-Box VX2 system, Version 15.06. Results: Native B. thuringiensis strains studied, showed high genetic diversity. 48,3% of the studied B. thuringiensis strains of the collection carry cry1 gene, 49,06% of them harbor both cry1 and cry2 gene, 5.7% of the native Bt strains didn?t react with any cry1 and cry 2 specific primers and 94.3% of the strains produce different PCR products. The analysis of cry1 positive B. thuringiensis showed sub-group frequencies of 7.6% for cry1F and 3.8% for cry1B and cry1C. In native Bt strains of our collection, the cry2 gene was always present with one or two cry1 sub-group(s). Conclusion: In this study, high genetic diversity in the native Bt strains from the bacterial collection in Mali was observed. Most of the native Bt strains studied harbor the cry1 gene only. In the cry1 gene profile, the cry1F sub-group was found to be the most frequently detected. None of studied Bt strains harbored the cry2 gene only. MenosAims: To determine the genetic diversity of Bacillus thuringiensis strains isolated from soils of different locality in Mali, and select strains with cry1F, cry1B and cry1C genes to control caterpillars and strains with cry2 gene against African rice gall midge. Study Design: Strains of Bacillus thuringiensis (Bt) used in this study belong to collection of Laboratory of Research in Microbiology and Microbial Biotechnology (Laborem-Biotech) and that isolated from different ecological environment of Mali. Bt strains used as positive controls were kindly provided by Dr. Fernando Hercos Valicente from Embrapa Milho e Sorgo (Brazil). Methodology: The total DNA of the native B. thuringiensis and reference strains were extracted from overnight grown culture. Gene identification was performed by amplification (PCR) of DNA of Bt strains using specific primers. The gel revelation was performed using ethidium bromide and the gel photography was performed using an E-Box VX2 system, Version 15.06. Results: Native B. thuringiensis strains studied, showed high genetic diversity. 48,3% of the studied B. thuringiensis strains of the collection carry cry1 gene, 49,06% of them harbor both cry1 and cry2 gene, 5.7% of the native Bt strains didn?t react with any cry1 and cry 2 specific primers and 94.3% of the strains produce different PCR products. The analysis of cry1 positive B. thuringiensis showed sub-group frequencies of 7.6% for cry1F and 3.8% for cry1B and cry1C. In native Bt strains of o... Mostrar Tudo |
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Palavras-Chave: |
Helicoverpa armegira. |
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Thesagro: |
Bacillus thuringiensis. |
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Categoria do assunto: |
-- |
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Marc: |
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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| Registros recuperados : 9 | |
| 1. |  | GIONBELLI, T. R. S.; VELOSO, C. M.; ROTTA, P. P.; VALADARES FILHO, S. C.; CARVALHO, B. C. de; M. I. MARCONDES; CUNHA, C. S.; NOVAES, M. A. S.; PREZOTTO, L. D.; DUARTE, M. S.; GIONBELLI, M. P. Foetal development of skeletal muscle in bovines as a function of maternal nutrition, foetal sex and gestational age. Journal of Animal Physiology and Animal Nutrition, v. 102, p. 545-556, 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 2. | | ROTTA, P. P.; VALADARES FILHO, S. C.; GIONBELLI, T. R. S.; COSTA E SILVA, L. F.; ENGLE, T. E.; MARCONDES, M. I.; MACHADO, F. S.; VILLADIEGO, F. A. C.; SILVA, J. C. J. Effects of day of gestation and feeding regimen in Holstein × Gyr cows: I. Apparent total-tract digestibility, nitrogen balance, and fat deposition. Journal of Dairy Science, v. 98, n. 5, p. 3197-3210, 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 3. | | ROTTA, P. P.; VALADARES FILHO, S. C.; GIONBELLI, T. R. S.; COSTA E SILVA, L. F.; ENGLE, T. E.; MARCONDES, M. I.; CAMPOS, M. M.; MENEZES, A. C. B.; LOBO, A. A. G. Effects of day of gestation and feeding regimen in Holstein × Gyr cows: II. maternal and fetal visceral organ mass. Journal of Dairy Science, v. 98, p. 3211-3223, 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 4. | | ROTTA, P. P.; VALADARES FILHO, S. C.; ENGLE, T. E.; SILVA, L. F. C. e; MARCONDES, M. I.; MACHADO, F. S.; GIONBELLI, T. R. S.; SILVA, B. C.; PACHECO, M. V. C. Effects of day of gestation and feeding regimen in Holstein x Gyr cows on apparent total-tract digestibility, nitrogen balance, and fat deposition. In: ADSA ASAS JOINT ANNUAL MEETING, 2015, Orlando. Proceedings... Orlando: ADSA: ASAS, 2015.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 5. | | ROTTA, P. P.; VALADARES FILHO, S. C.; ENGLE, T. E.; SILVA, L. F. C.; MARCONDES, M. I.; CAMPOS, M. M.; GIONBELLI, T. R. S.; SILVA, L. H. R.; MARTINS, E. C.; SILVA, F. A. S.; VILLADIEGO, F. A. C. Effects of day gestation and feeding regimen in Holstein x Gyr cows on maternal and fetal visceral organ mass. In: ADSA ASAS JOINT ANNUAL MEETING, 2015, Orlando. Proceedings... Orlando: ADSA: ASAS, 2015.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 6. | | ROCHA, R. F. B.; BAENA, M. M.; ESTOPA, A. de C.; GERVASIO, I. C.; IBELLI, A. M. G.; GIONBELLI, T. R. S.; GIONBELLI, M. P.; FREITAS, R. T. F. de; MEIRELLES, S. L. C. Differential expression of HSF1 and HSPA6 genes and physiological responses in Angus and Simmental cattle breeds. Journal of Thermal Biology, v. 84, p. 92-98, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 7. | | GIONBELLI, T. R. S.; ROTTA, P. P.; VELOSO, C. M.; MARCONDES, M. I.; VALADARES FILHO, S. C.; CARVALHO, B. C. de; LOVATTI, J. V. R.; CUNHA, C. S.; NOVAIS, M. A. S.; DUARTE, M. S.; GIONBELLI, M. P. Investigation of effects of maternal nutrition intensification and fetal sex on development of skeletal muscle of bovine fetuses. In: ADSA ASAS JOINT ANNUAL MEETING, 2015, Orlando. Proceedings... Orlando: ADSA: ASAS, 2015.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 8. | | ROTTA, P. P.; VALADARES FILHO, S. C.; GIONBELLI, T. R. S.; COSTA E SILVA, L. F.; ENGLE, T. E.; MARCONDES, M. I.; GUIMARÃES, S. E. F.; NASCIMENTO, C. S.; CARVALHO, B. C. de; SILVA, F. A. S.; OLIVEIRA, J. R. S. Effects of day of gestation and feeding regimen in Holstein × Gyr cows: III. Placental adaptations and placentome gene expression. Journal of Dairy Science, v. 98, p. 3224-3235, 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 9. | | GIONBELLI, T. R. S.; ROTTA, P. P.; VELOSO, C. M.; VALADARES FILHO, S. C.; CARVALHO, B. C. de; MARCONDES, M. I.; FERREIRA, M. F. L.; SOUZA, J. V. F.; SANTOS, J. S. A. A.; LACERDA, L. C.; DUARTE, M. S.; GIONBELLI, M. P. Intestinal development of bovine foetuses during gestation is affected by foetal sex and maternal nutrition. Journal of Animal Physiology and Animal Nutrition, v. 101, n. 3, p. 493-501, 2017. Publicado online em 16 ago. 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| Registros recuperados : 9 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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