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Registro Completo |
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
18/12/2015 |
Data da última atualização: |
03/11/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
MERTZ-HENNING, L. M.; NAGASHIMA, A. I.; KRZYZANOWSKI, F. C.; BINNECK, E.; HENNING, F. A. |
Afiliação: |
LILIANE MARCIA MERTZ HENNING, CNPSO; FRANCISCO CARLOS KRZYZANOWSKI, CNPSO; ELISEU BINNECK, CNPSO; FERNANDO AUGUSTO HENNING, CNPSO. |
Título: |
Relative quantification of gene expression levels associated with lignin biosynthesis in soybean seed coat. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
Seed Science and Technology, Zurich, v. 43, n. 3, p. 445-455, Dec. 2015. |
ISSN: |
0251-0952 |
DOI: |
10.15258/sst.2015.43.3.13 |
Idioma: |
Inglês |
Conteúdo: |
There is genetic variability among soybean genotypes for seed quality, which may be related to seed coat characteristics. An example of this is the variable lignin content in the seed coat, a trait associated with tolerance to mechanical damage and to deterioration caused by high relative humidity. The aim of this study was to evaluate soybean seed quality and determine the relative expression of genes associated with lignin biosynthesis in soybean seed coats. Two soybean cultivars differing with respect to lignin content in the seed coat, Doko (high) and Savana (low) were studied. Evaluations were carried out on seeds of four different sizes, representing the development stages of the seeds. Seed quality was evaluated by germination, accelerated ageing and electrical conductivity tests. Through real-time PCR, relative quantification (Doko/Savana) of the genes that encode PAL, C4H, 4CL, HCT, CCoAOMT, CCR, COMT and CAD enzymes was carried out, using the ?-Actin gene as an endogenous control. According to the results of the electrical conductivity test, Doko cultivar with high lignin content has the best seed coat structure. PAL, CCoA, 4Cl and CCR genes were upregulated in Doko cultivar relative to the Savana cultivar. |
Thesagro: |
Genótipo; Qualidade; Semente; Soja. |
Thesaurus Nal: |
Genotype; Seed quality; Soybeans. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
Marc: |
LEADER 02046naa a2200277 a 4500 001 2031997 005 2017-11-03 008 2015 bl uuuu u00u1 u #d 022 $a0251-0952 024 7 $a10.15258/sst.2015.43.3.13$2DOI 100 1 $aMERTZ-HENNING, L. M. 245 $aRelative quantification of gene expression levels associated with lignin biosynthesis in soybean seed coat.$h[electronic resource] 260 $c2015 520 $aThere is genetic variability among soybean genotypes for seed quality, which may be related to seed coat characteristics. An example of this is the variable lignin content in the seed coat, a trait associated with tolerance to mechanical damage and to deterioration caused by high relative humidity. The aim of this study was to evaluate soybean seed quality and determine the relative expression of genes associated with lignin biosynthesis in soybean seed coats. Two soybean cultivars differing with respect to lignin content in the seed coat, Doko (high) and Savana (low) were studied. Evaluations were carried out on seeds of four different sizes, representing the development stages of the seeds. Seed quality was evaluated by germination, accelerated ageing and electrical conductivity tests. Through real-time PCR, relative quantification (Doko/Savana) of the genes that encode PAL, C4H, 4CL, HCT, CCoAOMT, CCR, COMT and CAD enzymes was carried out, using the ?-Actin gene as an endogenous control. According to the results of the electrical conductivity test, Doko cultivar with high lignin content has the best seed coat structure. PAL, CCoA, 4Cl and CCR genes were upregulated in Doko cultivar relative to the Savana cultivar. 650 $aGenotype 650 $aSeed quality 650 $aSoybeans 650 $aGenótipo 650 $aQualidade 650 $aSemente 650 $aSoja 700 1 $aNAGASHIMA, A. I. 700 1 $aKRZYZANOWSKI, F. C. 700 1 $aBINNECK, E. 700 1 $aHENNING, F. A. 773 $tSeed Science and Technology, Zurich$gv. 43, n. 3, p. 445-455, Dec. 2015.
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Embrapa Soja (CNPSO) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Agroindústria de Alimentos. Para informações adicionais entre em contato com ctaa.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Agroindústria de Alimentos. |
Data corrente: |
25/11/2002 |
Data da última atualização: |
25/11/2002 |
Autoria: |
CARVALHO, L. M. J.; CABRAL, L. M. C.; SILVA, C. A. B.; ABADIO, F. D. B. |
Título: |
Clarificacao por ultrafiltracao de suco de abacaxi (Ananas comosus L.) |
Ano de publicação: |
1999 |
Fonte/Imprenta: |
In: AGROTEC 99, Sao Paulo, SP. Advance program.... Sao Paulo, 1999. p.21. |
Idioma: |
Português |
Palavras-Chave: |
Frutas; Ultrafiltracao. |
Thesagro: |
Abacaxi; Alimento Preparado; Clarificação; Suco. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00618naa a2200217 a 4500 001 1413774 005 2002-11-25 008 1999 bl --- 0-- u #d 100 1 $aCARVALHO, L. M. J. 245 $aClarificacao por ultrafiltracao de suco de abacaxi (Ananas comosus L.) 260 $c1999 650 $aAbacaxi 650 $aAlimento Preparado 650 $aClarificação 650 $aSuco 653 $aFrutas 653 $aUltrafiltracao 700 1 $aCABRAL, L. M. C. 700 1 $aSILVA, C. A. B. 700 1 $aABADIO, F. D. B. 773 $tIn: AGROTEC 99, Sao Paulo, SP. Advance program.... Sao Paulo, 1999. p.21.
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