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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
05/07/2023 |
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Data da última atualização: |
10/04/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
HIMMEN, F. D. A.; SOUZA, F. A. de; SILVA-CARDOSO, I. M. de A.; SOUZA, A. L. X. de; PEREIRA, J. E. S. |
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Afiliação: |
FERNANDA DUARTE ARAÚJO HIMMEN, UNIVERSIDADE DE BRASILIA; FRANCISCO ADRIANO DE SOUZA, CNPMS; INAÊ MARIÊ DE ARAÚJO SILVA-CARDOSO; ANDRE LUIS XAVIER DE SOUZA, Cenargen; JONNY EVERSON SCHERWINSKI PEREIRA, Cenargen. |
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Título: |
Micropropagation, estimation of DNA methylation during multiplication cycles and mycorrhization of seed-derived Dendrocalamus asper (Schultes f.) Backer ex Heyne. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Plant Cell, Tissue and Organ Culture, v. 155, p. 41-56, 2023. |
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DOI: |
https://doi.org/10.1007/s11240-023-02547-x |
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Idioma: |
Inglês |
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Conteúdo: |
Considering the economic significance of Dendrocalamus asper, a woody bamboo species, the objective of this study was to establish an in vitro micropropagation and mycorrhization protocol, and to analyze the DNA methylation dynamics during the multiplication stage of plants of this species. For shoot proliferation, meta-topolin (mT), 6-benzylaminopurine (BAP) and kinetin (Kin) were evaluated at different concentrations (0, 3.3, 6.6 and 13.3 µM) during three monthly subcultures. For in vitro mycorrhization, Rhizoglomus clarum inoculated in liquid medium was used. Murashige and Skoog (MS) and Strullu and Romand (MSR) culture media were tested with different modifications, such as reduced phosphorus, sucrose, and salts. The evidence of root colonization (spores, hyphae, or vesicles) was assessed. For micropropagation, it was verified that mT was as efficient as BAP for the in vitro multiplication of D. asper shoots. There was also a substantial reduction in the number of shoots in the third subculture, which coincided with a significant decrease in DNA methylation evaluated via ELISA, unprecedented analysis carried out during bamboo micropropagation. As for mycorrhization, colonization was observed only in plants grown on MSR (16% of the plants) and MS/2 medium with 25% of the total amount of sucrose (25% of the plants). These results will support the development of efficient in vitro mycorrhization protocols for D. asper and, consequently, the optimization of the quality of the micropropagated plantlets. MenosConsidering the economic significance of Dendrocalamus asper, a woody bamboo species, the objective of this study was to establish an in vitro micropropagation and mycorrhization protocol, and to analyze the DNA methylation dynamics during the multiplication stage of plants of this species. For shoot proliferation, meta-topolin (mT), 6-benzylaminopurine (BAP) and kinetin (Kin) were evaluated at different concentrations (0, 3.3, 6.6 and 13.3 µM) during three monthly subcultures. For in vitro mycorrhization, Rhizoglomus clarum inoculated in liquid medium was used. Murashige and Skoog (MS) and Strullu and Romand (MSR) culture media were tested with different modifications, such as reduced phosphorus, sucrose, and salts. The evidence of root colonization (spores, hyphae, or vesicles) was assessed. For micropropagation, it was verified that mT was as efficient as BAP for the in vitro multiplication of D. asper shoots. There was also a substantial reduction in the number of shoots in the third subculture, which coincided with a significant decrease in DNA methylation evaluated via ELISA, unprecedented analysis carried out during bamboo micropropagation. As for mycorrhization, colonization was observed only in plants grown on MSR (16% of the plants) and MS/2 medium with 25% of the total amount of sucrose (25% of the plants). These results will support the development of efficient in vitro mycorrhization protocols for D. asper and, consequently, the optimization of the quality of th... Mostrar Tudo |
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Palavras-Chave: |
Arbuscular mycorrhizal fungi; Bambusoideae; Epigenética; Fungo micorrízico arbuscular; In vitro propagation; Propagação in vitro. |
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Thesagro: |
Bambu; DNA; Fungo; Micorriza; Semente. |
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Thesaurus Nal: |
Bamboos; Epigenetics. |
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Categoria do assunto: |
--
G Melhoramento Genético |
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Marc: |
LEADER 02599naa a2200337 a 4500
001 2154816
005 2024-04-10
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s11240-023-02547-x$2DOI
100 1 $aHIMMEN, F. D. A.
245 $aMicropropagation, estimation of DNA methylation during multiplication cycles and mycorrhization of seed-derived Dendrocalamus asper (Schultes f.) Backer ex Heyne.$h[electronic resource]
260 $c2023
520 $aConsidering the economic significance of Dendrocalamus asper, a woody bamboo species, the objective of this study was to establish an in vitro micropropagation and mycorrhization protocol, and to analyze the DNA methylation dynamics during the multiplication stage of plants of this species. For shoot proliferation, meta-topolin (mT), 6-benzylaminopurine (BAP) and kinetin (Kin) were evaluated at different concentrations (0, 3.3, 6.6 and 13.3 µM) during three monthly subcultures. For in vitro mycorrhization, Rhizoglomus clarum inoculated in liquid medium was used. Murashige and Skoog (MS) and Strullu and Romand (MSR) culture media were tested with different modifications, such as reduced phosphorus, sucrose, and salts. The evidence of root colonization (spores, hyphae, or vesicles) was assessed. For micropropagation, it was verified that mT was as efficient as BAP for the in vitro multiplication of D. asper shoots. There was also a substantial reduction in the number of shoots in the third subculture, which coincided with a significant decrease in DNA methylation evaluated via ELISA, unprecedented analysis carried out during bamboo micropropagation. As for mycorrhization, colonization was observed only in plants grown on MSR (16% of the plants) and MS/2 medium with 25% of the total amount of sucrose (25% of the plants). These results will support the development of efficient in vitro mycorrhization protocols for D. asper and, consequently, the optimization of the quality of the micropropagated plantlets.
650 $aBamboos
650 $aEpigenetics
650 $aBambu
650 $aDNA
650 $aFungo
650 $aMicorriza
650 $aSemente
653 $aArbuscular mycorrhizal fungi
653 $aBambusoideae
653 $aEpigenética
653 $aFungo micorrízico arbuscular
653 $aIn vitro propagation
653 $aPropagação in vitro
700 1 $aSOUZA, F. A. de
700 1 $aSILVA-CARDOSO, I. M. de A.
700 1 $aSOUZA, A. L. X. de
700 1 $aPEREIRA, J. E. S.
773 $tPlant Cell, Tissue and Organ Culture$gv. 155, p. 41-56, 2023.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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Biblioteca |
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Registro Completo
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
22/06/2020 |
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Data da última atualização: |
22/06/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
BARROS, B. G. de F.; FREITAS, A. D. S. de; TABOSA, J. N.; LYRA, M. do C. C. P. de; MERGULHÃO, A. C. do E. S.; SILVA, A. F. da; OLIVEIRA, W. da S.; FERNANDES JUNIOR, P. I.; SAMPAIO, E. V. de S. B. |
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Afiliação: |
Benaia Gonçalves de Franca Barros; Ana Dolores Santiago de Freitas; Jose Nildo Tabosa; Maria do Carmo Catanho Pereira de Lyra; Adalia Cavalcanti do Espirito Santo Mergulhão; Aleksandro Ferreira da Silva; Wagner da Silva Oliveira; PAULO IVAN FERNANDES JUNIOR, CPATSA; Everardo Valadares de Sá Barretto Sampaio. |
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Título: |
Biological nitrogen fixation in field-grown sorghum under different edaphoclimatic conditions is confirmed by N isotopic signatures. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Nutrient Cycling in Agroecosystems, v. 117, p. 93-101, 2020. |
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DOI: |
https://doi.org/10.1007/s10705-020-10051-3 |
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Idioma: |
Inglês |
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Conteúdo: |
The association between sorghum and N2-fixing bacteria has been assessed only under limited conditions. We investigated biological nitrogen fixation (BNF) in situ in fifteen sorghum genotypes with dry or succulent culm types under five edaphoclimatic conditions. One randomized block experiment was established in each of five locations, from the humid to the semiarid regions of Pernambuco state, Brazil. BNF was estimated using the 15N natural abundance method by comparing the average d15N value of each sorghum genotype with those of the reference species. High levels of productivity, up to 22 Mg shoot biomass ha-1 in the 3-month cycle, were obtained where rainfall was high, and up to 5 Mg ha-1 was obtained under low rainfall. The nitrogen contents showed a similar pattern as biomass production, and the genotypes with the highest productivity accumulated from 200 to 300 kg N ha-1. BNF ranged from 55 to 78% of plant N in one location and from 36 to 56% in another location, but BNF did not occur in the other three locations. Although the factors that blocked effective symbiosis were not determined, symbiosis was not influenced by P or K availability. The proportion of N2 fixation was similar in the grainproducing, dry culm genotypes and in the sugar-rich, succulent culm genotypes. The sorghum genotypes fixed N2, reaching up to 218 kg ha-1 N, without inoculation with diazotrophs. Therefore, sorghum has a high potential to fix atmospheric N2, but the factors that block N2 fixation must be identified for crop management planning. MenosThe association between sorghum and N2-fixing bacteria has been assessed only under limited conditions. We investigated biological nitrogen fixation (BNF) in situ in fifteen sorghum genotypes with dry or succulent culm types under five edaphoclimatic conditions. One randomized block experiment was established in each of five locations, from the humid to the semiarid regions of Pernambuco state, Brazil. BNF was estimated using the 15N natural abundance method by comparing the average d15N value of each sorghum genotype with those of the reference species. High levels of productivity, up to 22 Mg shoot biomass ha-1 in the 3-month cycle, were obtained where rainfall was high, and up to 5 Mg ha-1 was obtained under low rainfall. The nitrogen contents showed a similar pattern as biomass production, and the genotypes with the highest productivity accumulated from 200 to 300 kg N ha-1. BNF ranged from 55 to 78% of plant N in one location and from 36 to 56% in another location, but BNF did not occur in the other three locations. Although the factors that blocked effective symbiosis were not determined, symbiosis was not influenced by P or K availability. The proportion of N2 fixation was similar in the grainproducing, dry culm genotypes and in the sugar-rich, succulent culm genotypes. The sorghum genotypes fixed N2, reaching up to 218 kg ha-1 N, without inoculation with diazotrophs. Therefore, sorghum has a high potential to fix atmospheric N2, but the factors that block N2 fixation... Mostrar Tudo |
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Palavras-Chave: |
Fixação biológica de nitrogênio; Técnica de abundância natural. |
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Thesagro: |
Bactéria; Fixação de Nitrogênio; Nitrogênio; Sorgo. |
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Thesaurus NAL: |
Biological control; Grain sorghum; Sorghum bicolor subsp. bicolor. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/214133/1/Biological-nitrogen-fixation-2020.pdf
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Marc: |
LEADER 02683naa a2200337 a 4500
001 2123405
005 2020-06-22
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s10705-020-10051-3$2DOI
100 1 $aBARROS, B. G. de F.
245 $aBiological nitrogen fixation in field-grown sorghum under different edaphoclimatic conditions is confirmed by N isotopic signatures.$h[electronic resource]
260 $c2020
520 $aThe association between sorghum and N2-fixing bacteria has been assessed only under limited conditions. We investigated biological nitrogen fixation (BNF) in situ in fifteen sorghum genotypes with dry or succulent culm types under five edaphoclimatic conditions. One randomized block experiment was established in each of five locations, from the humid to the semiarid regions of Pernambuco state, Brazil. BNF was estimated using the 15N natural abundance method by comparing the average d15N value of each sorghum genotype with those of the reference species. High levels of productivity, up to 22 Mg shoot biomass ha-1 in the 3-month cycle, were obtained where rainfall was high, and up to 5 Mg ha-1 was obtained under low rainfall. The nitrogen contents showed a similar pattern as biomass production, and the genotypes with the highest productivity accumulated from 200 to 300 kg N ha-1. BNF ranged from 55 to 78% of plant N in one location and from 36 to 56% in another location, but BNF did not occur in the other three locations. Although the factors that blocked effective symbiosis were not determined, symbiosis was not influenced by P or K availability. The proportion of N2 fixation was similar in the grainproducing, dry culm genotypes and in the sugar-rich, succulent culm genotypes. The sorghum genotypes fixed N2, reaching up to 218 kg ha-1 N, without inoculation with diazotrophs. Therefore, sorghum has a high potential to fix atmospheric N2, but the factors that block N2 fixation must be identified for crop management planning.
650 $aBiological control
650 $aGrain sorghum
650 $aSorghum bicolor subsp. bicolor
650 $aBactéria
650 $aFixação de Nitrogênio
650 $aNitrogênio
650 $aSorgo
653 $aFixação biológica de nitrogênio
653 $aTécnica de abundância natural
700 1 $aFREITAS, A. D. S. de
700 1 $aTABOSA, J. N.
700 1 $aLYRA, M. do C. C. P. de
700 1 $aMERGULHÃO, A. C. do E. S.
700 1 $aSILVA, A. F. da
700 1 $aOLIVEIRA, W. da S.
700 1 $aFERNANDES JUNIOR, P. I.
700 1 $aSAMPAIO, E. V. de S. B.
773 $tNutrient Cycling in Agroecosystems$gv. 117, p. 93-101, 2020.
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