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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
12/01/2009 |
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Data da última atualização: |
24/05/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
AARESTRUP, J. R.; KARAM, D.; FERNANDES, G. W. |
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Afiliação: |
Juliana Roriz Aarestrup, Bolsista da FAPEMIG; DECIO KARAM, CNPMS; Geraldo W. Fernandes, UFMG. |
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Título: |
Chromosome number and cytogenetics of Euphorbia heterophylla L. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
Genetics and Molecular Research, Ribeirão Preto, v. 7, n. 1, p. 217-222, 2008. |
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Idioma: |
Inglês |
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Conteúdo: |
Euphorbia heterophylla L. (Euphorbiaceae) is a herbaceous species of great economic importance due to its invasive potential and consequent damage to agriculture and pasture land. For the first time, we provide information on its chromosome nwnber, morphology, and behavior of mitotic chromosomes. Seeds were germinated and submitted to four treatments to obtain metaphases: 0.5% co1chicine for 2 to 5 h, at ambient temperature; 0.5% co1chicine for 16 to 24 h; 0.0029 M 8-hydroxyquinoline (8-HQ) for 2 to 5 h at ambient temperature, and 0.0029 M 8-HQ for 16 to 24 h at 4 degrees C. The material was then fixed in methanol: acetic acid (3: I) and kept at -20 degrees C for 24 h. Roots were macerated in the enzyme solution ofFlaxzyme (TM) (NOVO FERMENT T)-distilled water (I: 40) at 34 degrees C for 2 h and later fixed again. Chromosome preparations were obtained by the dissociation of the apical meristems. The best chromosome preparations were obtained with the use of 8-HQ for 21 h 30 min at 4 degrees C. E. heterophylla showed 2n = 28 chromosomes. The short arm of the largest pair of chromosomes of the complement (pair nwnber 1) displayed a secondary constriction while the nuc1eolus was observed in the interphasic cell. Structural rearrangements were also observed in the E. heterophylla L. genome. The genomic instability associated with polyploidy may be the result of selection shaped by environmental adaptations and/or human-induced manipulation through agricultural practices. |
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Palavras-Chave: |
Planta daninha. |
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Thesaurus Nal: |
evolution; polyploidy. |
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Categoria do assunto: |
V Taxonomia de Organismos |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/66645/1/Chromossome-number.pdf
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Marc: |
LEADER 02026naa a2200181 a 4500
001 1491644
005 2018-05-24
008 2008 bl uuuu u00u1 u #d
100 1 $aAARESTRUP, J. R.
245 $aChromosome number and cytogenetics of Euphorbia heterophylla L.$h[electronic resource]
260 $c2008
520 $aEuphorbia heterophylla L. (Euphorbiaceae) is a herbaceous species of great economic importance due to its invasive potential and consequent damage to agriculture and pasture land. For the first time, we provide information on its chromosome nwnber, morphology, and behavior of mitotic chromosomes. Seeds were germinated and submitted to four treatments to obtain metaphases: 0.5% co1chicine for 2 to 5 h, at ambient temperature; 0.5% co1chicine for 16 to 24 h; 0.0029 M 8-hydroxyquinoline (8-HQ) for 2 to 5 h at ambient temperature, and 0.0029 M 8-HQ for 16 to 24 h at 4 degrees C. The material was then fixed in methanol: acetic acid (3: I) and kept at -20 degrees C for 24 h. Roots were macerated in the enzyme solution ofFlaxzyme (TM) (NOVO FERMENT T)-distilled water (I: 40) at 34 degrees C for 2 h and later fixed again. Chromosome preparations were obtained by the dissociation of the apical meristems. The best chromosome preparations were obtained with the use of 8-HQ for 21 h 30 min at 4 degrees C. E. heterophylla showed 2n = 28 chromosomes. The short arm of the largest pair of chromosomes of the complement (pair nwnber 1) displayed a secondary constriction while the nuc1eolus was observed in the interphasic cell. Structural rearrangements were also observed in the E. heterophylla L. genome. The genomic instability associated with polyploidy may be the result of selection shaped by environmental adaptations and/or human-induced manipulation through agricultural practices.
650 $aevolution
650 $apolyploidy
653 $aPlanta daninha
700 1 $aKARAM, D.
700 1 $aFERNANDES, G. W.
773 $tGenetics and Molecular Research, Ribeirão Preto$gv. 7, n. 1, p. 217-222, 2008.
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