|
|
Registro Completo |
Biblioteca(s): |
Embrapa Milho e Sorgo. |
Data corrente: |
21/09/2017 |
Data da última atualização: |
14/04/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
DORNELAS, J. C. M.; FIGUEIREDO, J. E. F.; ABREU, C. S. de; LANA, U. G. P.; OLIVEIRA-PAIVA, C. A.; MARRIEL, I. E. |
Afiliação: |
Universidade Federal de São João Del Rei; JOSE EDSON FONTES FIGUEIREDO, CNPMS; Universidade Federal de Minas Gerais; UBIRACI GOMES DE PAULA LANA, CNPMS; CHRISTIANE ABREU DE OLIVEIRA PAIVA, CNPMS; IVANILDO EVODIO MARRIEL, CNPMS. |
Título: |
Characterization and phylogenetic affiliation of Actinobacteria from tropical soils with potential uses for agro-industrial processes. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
Genetics and Molecular Research, Ribeirão Preto, v. 16, n. 3, p. 1-16, 2017. |
DOI: |
10.4238/gmr16039703 |
Idioma: |
Inglês |
Conteúdo: |
Secondary metabolites produced by Actinobacteria of tropical soils represent a largely understudied source of novel molecules with relevant application in medicine, pharmaceutical and food industries, agriculture, and environmental bioremediation. The present study aimed to characterize sixty-nine Actinobacteria isolated from compost and tropical soils using morphological, biochemical, and molecular methods. All the isolates showed high variation for morphological traits considering the color of pigments of the aerial and vegetative mycelium and spore chain morphology. The enzymatic activity of amylase, cellulase, and lipase was highly variable. The amylase activity was detected in 53 (76.81%) isolates. Eighteen (Streptomyces curacoi) and ACSL 6 (S. hygroscopicus) showed the highest EI values (6.44 and 6.42, respectively). The cellulase activity varied significantly (P ≤ 0.05) among the isolates. Twenty-nine isolates (42.02%) showed high cellulase activity, and the isolates ACJ 48 (S. chiangmaiensis) and ACJ 53 (S. cyslabdanicus) showed the highest EI values (6.56 for both isolates). The lipase activity varied statistically (P ≤ 0.05) with fourteen isolates (20.29%) considered good lipase producers (EI > 2.0). The isolate ACSL 6 (S. hygroscopicus) showed the highest EI value of 2.60. Molecular analysis of partial 16S rRNA gene sequencing revealed the existence of 49 species, being 38 species with only one representative member and 11 species represented by one or more strains. All species belonged to three genera, namely Streptomyces (82.61%), Amycolatopsis (7.25%), and Kitasatospora (10.14%). The present results showed the high biotechnological potential of different Actinobacteria from tropical soils. MenosSecondary metabolites produced by Actinobacteria of tropical soils represent a largely understudied source of novel molecules with relevant application in medicine, pharmaceutical and food industries, agriculture, and environmental bioremediation. The present study aimed to characterize sixty-nine Actinobacteria isolated from compost and tropical soils using morphological, biochemical, and molecular methods. All the isolates showed high variation for morphological traits considering the color of pigments of the aerial and vegetative mycelium and spore chain morphology. The enzymatic activity of amylase, cellulase, and lipase was highly variable. The amylase activity was detected in 53 (76.81%) isolates. Eighteen (Streptomyces curacoi) and ACSL 6 (S. hygroscopicus) showed the highest EI values (6.44 and 6.42, respectively). The cellulase activity varied significantly (P ≤ 0.05) among the isolates. Twenty-nine isolates (42.02%) showed high cellulase activity, and the isolates ACJ 48 (S. chiangmaiensis) and ACJ 53 (S. cyslabdanicus) showed the highest EI values (6.56 for both isolates). The lipase activity varied statistically (P ≤ 0.05) with fourteen isolates (20.29%) considered good lipase producers (EI > 2.0). The isolate ACSL 6 (S. hygroscopicus) showed the highest EI value of 2.60. Molecular analysis of partial 16S rRNA gene sequencing revealed the existence of 49 species, being 38 species with only one representative member and 11 species represented by one or... Mostrar Tudo |
Palavras-Chave: |
Identificação molecular; Molecular identification; Morphological traits. |
Thesagro: |
Actinomiceto; Atividade enzimática. |
Thesaurus Nal: |
Actinobacteria; Enzyme activity. |
Categoria do assunto: |
S Ciências Biológicas |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/164140/1/Characterization-phylogenetic.pdf
|
Marc: |
LEADER 02669naa a2200277 a 4500 001 2076062 005 2021-04-14 008 2017 bl uuuu u00u1 u #d 024 7 $a10.4238/gmr16039703$2DOI 100 1 $aDORNELAS, J. C. M. 245 $aCharacterization and phylogenetic affiliation of Actinobacteria from tropical soils with potential uses for agro-industrial processes.$h[electronic resource] 260 $c2017 520 $aSecondary metabolites produced by Actinobacteria of tropical soils represent a largely understudied source of novel molecules with relevant application in medicine, pharmaceutical and food industries, agriculture, and environmental bioremediation. The present study aimed to characterize sixty-nine Actinobacteria isolated from compost and tropical soils using morphological, biochemical, and molecular methods. All the isolates showed high variation for morphological traits considering the color of pigments of the aerial and vegetative mycelium and spore chain morphology. The enzymatic activity of amylase, cellulase, and lipase was highly variable. The amylase activity was detected in 53 (76.81%) isolates. Eighteen (Streptomyces curacoi) and ACSL 6 (S. hygroscopicus) showed the highest EI values (6.44 and 6.42, respectively). The cellulase activity varied significantly (P ≤ 0.05) among the isolates. Twenty-nine isolates (42.02%) showed high cellulase activity, and the isolates ACJ 48 (S. chiangmaiensis) and ACJ 53 (S. cyslabdanicus) showed the highest EI values (6.56 for both isolates). The lipase activity varied statistically (P ≤ 0.05) with fourteen isolates (20.29%) considered good lipase producers (EI > 2.0). The isolate ACSL 6 (S. hygroscopicus) showed the highest EI value of 2.60. Molecular analysis of partial 16S rRNA gene sequencing revealed the existence of 49 species, being 38 species with only one representative member and 11 species represented by one or more strains. All species belonged to three genera, namely Streptomyces (82.61%), Amycolatopsis (7.25%), and Kitasatospora (10.14%). The present results showed the high biotechnological potential of different Actinobacteria from tropical soils. 650 $aActinobacteria 650 $aEnzyme activity 650 $aActinomiceto 650 $aAtividade enzimática 653 $aIdentificação molecular 653 $aMolecular identification 653 $aMorphological traits 700 1 $aFIGUEIREDO, J. E. F. 700 1 $aABREU, C. S. de 700 1 $aLANA, U. G. P. 700 1 $aOLIVEIRA-PAIVA, C. A. 700 1 $aMARRIEL, I. E. 773 $tGenetics and Molecular Research, Ribeirão Preto$gv. 16, n. 3, p. 1-16, 2017.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
Registro Completo
Biblioteca(s): |
Embrapa Amapá. |
Data corrente: |
27/09/2005 |
Data da última atualização: |
06/10/2022 |
Tipo da produção científica: |
Documentos |
Autoria: |
YOKOMIZO, G. K.-I. |
Afiliação: |
GILBERTO KEN ITI YOKOMIZO, CPAF-AP. |
Título: |
Relatório de gestão 2003. |
Ano de publicação: |
2004 |
Fonte/Imprenta: |
Macapá: Embrapa Amapá, 2004. |
Páginas: |
61 p. |
Série: |
(Embrapa Amapá. Documentos, 53). |
Idioma: |
Português |
Conteúdo: |
Perfil da unidade; Histórico da qualidade; Estratégias e planos; Melhoria de processos organizacionais. |
Palavras-Chave: |
Embrapa Amapá; Relatório. |
Thesagro: |
Gestão. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/CPAF-AP/8683/1/Documentos200453.PDF
|
Marc: |
LEADER 00523nam a2200169 a 4500 001 1348922 005 2022-10-06 008 2004 bl uuuu 00u1 u #d 100 1 $aYOKOMIZO, G. K.-I. 245 $aRelatório de gestão 2003. 260 $aMacapá: Embrapa Amapá$c2004 300 $a61 p. 490 $a(Embrapa Amapá. Documentos, 53). 520 $aPerfil da unidade; Histórico da qualidade; Estratégias e planos; Melhoria de processos organizacionais. 650 $aGestão 653 $aEmbrapa Amapá 653 $aRelatório
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Amapá (CPAF-AP) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Nenhum registro encontrado para a expressão de busca informada. |
|
|