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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
05/11/2014 |
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Data da última atualização: |
25/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PIO, L. A. S.; PASQUAL, M.; SILVA, S. de O. e; ROCHA, H. S.; MAGALHAES, H. M.; SEREJO, J. A. dos S. |
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Afiliação: |
LEILA APARECIDA SALLES PIO, UFLA; MOACIR PASQUAL, UFLA; SEBASTIAO DE OLIVEIRA E SILVA; HERMINIO SOUZA ROCHA, CNPMF; HELIDA MARA MAGALHAES, UNIPAR; JANAY ALMEIDA DOS SANTOS SEREJO, CNPMF. |
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Título: |
Inducing and identifying artificially-induced polyploidy in bananas. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
African Journal of Biotechnology , v.13, n.37, p. 3748-3758 , September 2014. |
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Idioma: |
Inglês |
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Conteúdo: |
In the present work, polyploidy was induced in the diploid banana varieties 'Malbut', 'Gold', 'Lidi', and 'Thong Dok Mak' through the use of colchicine and oryzalin, and that condition was identified through stomatal analysis, flow cytometry, and chromosome counts. Shoots produced in vitro were treated with colchicine at concentrations of 0, 2.5, 7.5 and 12.5 mM for 24 and 48 h, and with oryzalin at 0, 10, 30 and 50 mM for 4 and 7 days. Young leaves were scanned by electron microscopy to determine their stomatal areas (polar diameter × equatorial diameter) and numbers for polyploid identification by stomatal analysis. Polyploid identification by way of flow cytometry analysis used samples of young leaves that were crushed to release their nuclei, with subsequent staining with propidium iodide; ten thousand nuclei were analyzed for each sample. For cytogenetic analyses, root tips were pretreated with 0.002 M 8-HQ for 3 h, fixed in Carnoy solution for 24 h, subjected to conventional squashing techniques, and stained with 10% Giemsa. We identified four tetraploid plants and six mixoploids using these three identification techniques. |
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Palavras-Chave: |
Banan Thong Dok Mak; Banana Gold; Banana Lidi; Banana Malbut; Chromosomes duplication; Duplicação cormossômica. |
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Thesagro: |
Cultura de tecido; Musa Acuminata. |
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Thesaurus Nal: |
bananas; Tissue culture. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02025naa a2200301 a 4500
001 1999243
005 2023-05-25
008 2014 bl uuuu u00u1 u #d
100 1 $aPIO, L. A. S.
245 $aInducing and identifying artificially-induced polyploidy in bananas.$h[electronic resource]
260 $c2014
520 $aIn the present work, polyploidy was induced in the diploid banana varieties 'Malbut', 'Gold', 'Lidi', and 'Thong Dok Mak' through the use of colchicine and oryzalin, and that condition was identified through stomatal analysis, flow cytometry, and chromosome counts. Shoots produced in vitro were treated with colchicine at concentrations of 0, 2.5, 7.5 and 12.5 mM for 24 and 48 h, and with oryzalin at 0, 10, 30 and 50 mM for 4 and 7 days. Young leaves were scanned by electron microscopy to determine their stomatal areas (polar diameter × equatorial diameter) and numbers for polyploid identification by stomatal analysis. Polyploid identification by way of flow cytometry analysis used samples of young leaves that were crushed to release their nuclei, with subsequent staining with propidium iodide; ten thousand nuclei were analyzed for each sample. For cytogenetic analyses, root tips were pretreated with 0.002 M 8-HQ for 3 h, fixed in Carnoy solution for 24 h, subjected to conventional squashing techniques, and stained with 10% Giemsa. We identified four tetraploid plants and six mixoploids using these three identification techniques.
650 $abananas
650 $aTissue culture
650 $aCultura de tecido
650 $aMusa Acuminata
653 $aBanan Thong Dok Mak
653 $aBanana Gold
653 $aBanana Lidi
653 $aBanana Malbut
653 $aChromosomes duplication
653 $aDuplicação cormossômica
700 1 $aPASQUAL, M.
700 1 $aSILVA, S. de O. e
700 1 $aROCHA, H. S.
700 1 $aMAGALHAES, H. M.
700 1 $aSEREJO, J. A. dos S.
773 $tAfrican Journal of Biotechnology$gv.13, n.37, p. 3748-3758 , September 2014.
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