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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
07/07/2014 |
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Data da última atualização: |
16/01/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
VIDAL, Á. M.; COSTA, M. A. P. de C.; SOUZA, A. da S.; ALMEIDA, W. A. B. de; SOUZA, F. V. D. |
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Afiliação: |
ÁDILA MELO VIDAL, UFRB; MARIA ANGÉLICA PEREIRA DE CARVALHO COSTA, UFRB; ANTONIO DA SILVA SOUZA, CNPMF; WELITON ANTONIO BASTOS DE ALMEIDA, FAMAM; FERNANDA VIDIGAL DUARTE SOUZA, CNPMF. |
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Título: |
In vitro regeneration and morphogenesis of somatic embryos of cassava. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Revista Ciência Agronômica, v. 45, n. 3, p. 558-565, jul-set, 2014. |
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ISSN: |
1806-6690 |
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Idioma: |
Inglês |
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Conteúdo: |
In this study, the somatic embryogenesis and regeneration of Cigana Preta plants from shoot apices and immature leaves taken from plants cultivated in vitro were examined. To embryo induction the explants were cultivated in Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) or 4-amino-3,5,6-trichloropicolinic acid (Picloram) at concentrations of 8.0 and 12 mg L-1. To development of embryos two media cultures with different concentration of BAP (D1 or D2) were evaluated. Embryos in the cotyledonary stage were incubated in germination medium containing MS salts and vitamins, 2.0 ?M copper sulfate, 2.4 g L-1 of Phytagel® and 1.77 ?M BAP. The highest frequency of calluses and the greatest number of embryos per explant were obtained using the auxin picloram at a concentration of 8.0 mg L-1. The plants regenerated in the picloram treatment exhibited normal development and were transferred to a multiplication medium after a minimum of four weeks. The histological sections of the malformed embryos from foliar explants cultured in the presence of 2,4-D demonstrated that the origin of the cotyledonary structures was independent of the formation of the shoot apical meristem, which was not formed in the embryos, and the majority of the embryos were classified as cornet-shaped. This study demonstrates that in cassava, the use of different auxins provides different conditions for the formation of somatic embryos, and the low rate of conversion into plants results from abnormalities in the embryos. MenosIn this study, the somatic embryogenesis and regeneration of Cigana Preta plants from shoot apices and immature leaves taken from plants cultivated in vitro were examined. To embryo induction the explants were cultivated in Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) or 4-amino-3,5,6-trichloropicolinic acid (Picloram) at concentrations of 8.0 and 12 mg L-1. To development of embryos two media cultures with different concentration of BAP (D1 or D2) were evaluated. Embryos in the cotyledonary stage were incubated in germination medium containing MS salts and vitamins, 2.0 ?M copper sulfate, 2.4 g L-1 of Phytagel® and 1.77 ?M BAP. The highest frequency of calluses and the greatest number of embryos per explant were obtained using the auxin picloram at a concentration of 8.0 mg L-1. The plants regenerated in the picloram treatment exhibited normal development and were transferred to a multiplication medium after a minimum of four weeks. The histological sections of the malformed embryos from foliar explants cultured in the presence of 2,4-D demonstrated that the origin of the cotyledonary structures was independent of the formation of the shoot apical meristem, which was not formed in the embryos, and the majority of the embryos were classified as cornet-shaped. This study demonstrates that in cassava, the use of different auxins provides different conditions for the formation of somatic embryos, and the low rate of conversion into pl... Mostrar Tudo |
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Palavras-Chave: |
Cigana Preta. |
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Thesagro: |
Embriogênese; Mandioca. |
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Thesaurus Nal: |
Cassava; Plant growth substances; Somatic embryogenesis. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02261naa a2200253 a 4500
001 1989891
005 2015-01-16
008 2014 bl uuuu u00u1 u #d
022 $a1806-6690
100 1 $aVIDAL, Á. M.
245 $aIn vitro regeneration and morphogenesis of somatic embryos of cassava.
260 $c2014
520 $aIn this study, the somatic embryogenesis and regeneration of Cigana Preta plants from shoot apices and immature leaves taken from plants cultivated in vitro were examined. To embryo induction the explants were cultivated in Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) or 4-amino-3,5,6-trichloropicolinic acid (Picloram) at concentrations of 8.0 and 12 mg L-1. To development of embryos two media cultures with different concentration of BAP (D1 or D2) were evaluated. Embryos in the cotyledonary stage were incubated in germination medium containing MS salts and vitamins, 2.0 ?M copper sulfate, 2.4 g L-1 of Phytagel® and 1.77 ?M BAP. The highest frequency of calluses and the greatest number of embryos per explant were obtained using the auxin picloram at a concentration of 8.0 mg L-1. The plants regenerated in the picloram treatment exhibited normal development and were transferred to a multiplication medium after a minimum of four weeks. The histological sections of the malformed embryos from foliar explants cultured in the presence of 2,4-D demonstrated that the origin of the cotyledonary structures was independent of the formation of the shoot apical meristem, which was not formed in the embryos, and the majority of the embryos were classified as cornet-shaped. This study demonstrates that in cassava, the use of different auxins provides different conditions for the formation of somatic embryos, and the low rate of conversion into plants results from abnormalities in the embryos.
650 $aCassava
650 $aPlant growth substances
650 $aSomatic embryogenesis
650 $aEmbriogênese
650 $aMandioca
653 $aCigana Preta
700 1 $aCOSTA, M. A. P. de C.
700 1 $aSOUZA, A. da S.
700 1 $aALMEIDA, W. A. B. de
700 1 $aSOUZA, F. V. D.
773 $tRevista Ciência Agronômica$gv. 45, n. 3, p. 558-565, jul-set, 2014.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| 3. |  | PORFIRIO-DA-SILVA, V.; BAGGIO, A. J.; RIBASKI, J.; VARELLA, A. C.; PACIULLO, D. S. C.; NICODEMO, M. L. F. Los sistemas silvopastoriles en las regiones subtropicales del Brasil: las actividades de la Embrapa. In: CONGRESO NACIONAL DE SISTEMAS SILVOPASTORILES, 1., 2009, Posadas. Actas... Buenos Aires: INTA, 2009. p. 59-65.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Pecuária Sul. |
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| 4. | | VARELLA, A. C.; PORFIRIO-DA-SILVA, V.; RIBASKI, J.; SOARES, A. B.; MORAES, A.; MORAIS, H.; SAIBRO, J. C. de; BARRO, R. S. Estabelecimento de plantas forrageiras em sistemas de integração floresta-pecuária no Sul do Brasil. In: FONTANELI, R. S.; SANTOS, H. P. dos; FONTANELI, R. S. (Ed.). Forrageiras para integração lavoura-pecuária-floresta na região sul-brasileira. Passo Fundo: Embrapa Trigo, 2009. p. 283-303.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Pecuária Sul. |
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| 5. | | VARELLA, A. C.; PORFIRIO-DA-SILVA, V.; RIBASKI, J.; SOARES, A. B.; MORAES, A. de; MORAIS, H.; SAIBRO, J. C. de; BARRO, R. S. Estabelecimento de plantas forrageiras em sistemas de integração floresta-pecuária no Sul do Brasil. 2. ed. In: FONTANELI, R. S.; SANTOS, H. P. dos; FONTANELI, R. S. (Ed.). Forrageiras para integração lavoura-pecuária-floresta na região sul-brasileira. Brasília, DF: Embrapa, 2012. p. 435-460.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Florestas; Embrapa Pecuária Sul. |
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| 6. | | MAIA, M. B.; VARELLA, A. C.; LAMPERT, V. do N.; RIBASKI, J.; TADEO, R. W. M.; FRANTZ, A. C. da L.; VIEIRA, I. M. B. P. Economic evaluation of integrated livestock-forest systemat the establishment phase in the Southern region of Rio Grande do Sul - Brazil. In: WORLD CONGRESS ON INTEGRATED CROP-LIVESTOCK-FOREST SYSTEMS; INTERNATIONAL SYMPOSIUM ON INTEGRATED CROP-LIVESTOCK SYSTEMS, 3., 2015, Brasília, DF. Towards sustainable intensification: proceedings. Brasília, DF: Embrapa, 2015.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Florestas; Embrapa Pecuária Sul. |
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| 7. | | VARELLA, A. C.; RIBASKI, J.; SILVA, V. P.; SOARES, A. B.; MORAES, A.; MORAIS, H.; SAIBRO, J. C.; BARRO, R. S.; POLI, C. H. E. C.; PAULINO, B. M. Recomendações para a escolha e manejo de plantas forrageiras em sistemas silvipastoris no Sul do Brasil. Bagé: Embrapa Pecuária Sul, 2008. 25 p. il. color. (Embrapa Pecuária Sul. Documentos, 76). Disponível no formato online.| Biblioteca(s): Embrapa Florestas; Embrapa Pecuária Sul. |
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| Registros recuperados : 7 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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