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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
28/04/2014 |
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Data da última atualização: |
25/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ALFENAS, R. F.; PEREIRA, O. L.; FREITAS, R. G.; FREITAS, C. S.; RODRIGUEZ, M. A. D.; ALFENAS, A. C. |
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Afiliação: |
RAFAEL F. ALFENAS, UFV; OLINTO L. PEREIRA, UFV; RODRIGO G. FREITAS, UFV; CAMILA S. FREITAS, UFV; MIGUEL ANGEL DITA RODRIGUEZ, CNPMF; ACELINO C. ALFENAS, UFV. |
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Título: |
Mass spore production and inoculation of Calonectria pteridis on Eucalyptus spp. under different environmental conditions. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Tropical Plant Pathology, v. 38, n.5, p. 406-413, 2013. |
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ISSN: |
Cylindrocladium |
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Idioma: |
Inglês |
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Conteúdo: |
Calonectria pteridis is one of the most important causal agents of Calonectria leaf blight (CLB) of Eucalyptus spp. in Brazil, which is a limiting factor for growth of Eucalyptus plantations. In this study we developed standard procedures for in vitro mass spore production and inoculation of C. pteridis for screening plant species and clones for resistance to CLB. The isolates used in this study were identified using similarity in DNA sequence to reference strains. Among six media tested, glucose asparagine agar induced the highest sporulation production and a significantly enhanced sporulation was found by scraping the aerial mycelium and temporarily submersing the cultures in tap water, followed by drying and additional incubation of the culture. We also demonstrated that the severity of CLB on excised leaves of two Eucalyptus spp. clones increased significantly when the inoculated leaves were incubated in the dark. The optimal temperature for infection was 26ºC when plants were incubated for 48 hours post-inoculation in a mist chamber and then maintained in a greenhouse (25ºC±5ºC) for 50 days. This study identifies environmental conditions to improve spore production and inoculation procedures of C. pteridis for selection of resistant Eucalyptus spp. |
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Palavras-Chave: |
Forest pathology. |
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Thesagro: |
Eucalipto. |
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Thesaurus Nal: |
Cylindrocladium; Hypocreales; Leaf blight; Sporulation. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02069naa a2200265 a 4500
001 1985175
005 2023-05-25
008 2013 bl uuuu u00u1 u #d
022 $aCylindrocladium
100 1 $aALFENAS, R. F.
245 $aMass spore production and inoculation of Calonectria pteridis on Eucalyptus spp. under different environmental conditions.$h[electronic resource]
260 $c2013
520 $aCalonectria pteridis is one of the most important causal agents of Calonectria leaf blight (CLB) of Eucalyptus spp. in Brazil, which is a limiting factor for growth of Eucalyptus plantations. In this study we developed standard procedures for in vitro mass spore production and inoculation of C. pteridis for screening plant species and clones for resistance to CLB. The isolates used in this study were identified using similarity in DNA sequence to reference strains. Among six media tested, glucose asparagine agar induced the highest sporulation production and a significantly enhanced sporulation was found by scraping the aerial mycelium and temporarily submersing the cultures in tap water, followed by drying and additional incubation of the culture. We also demonstrated that the severity of CLB on excised leaves of two Eucalyptus spp. clones increased significantly when the inoculated leaves were incubated in the dark. The optimal temperature for infection was 26ºC when plants were incubated for 48 hours post-inoculation in a mist chamber and then maintained in a greenhouse (25ºC±5ºC) for 50 days. This study identifies environmental conditions to improve spore production and inoculation procedures of C. pteridis for selection of resistant Eucalyptus spp.
650 $aCylindrocladium
650 $aHypocreales
650 $aLeaf blight
650 $aSporulation
650 $aEucalipto
653 $aForest pathology
700 1 $aPEREIRA, O. L.
700 1 $aFREITAS, R. G.
700 1 $aFREITAS, C. S.
700 1 $aRODRIGUEZ, M. A. D.
700 1 $aALFENAS, A. C.
773 $tTropical Plant Pathology$gv. 38, n.5, p. 406-413, 2013.
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| 1. |  | VIANA, E. S.; CAMPOS, M. E. M.; PONCE, A. R.; MANTOVANI, H. C.; VANETTI, M. C. D. Detecção de moléculas sinalizadoras de quorum sensing em biofilmes formados por Hafnia alvei isoladas de leite. In: SIMPÓSIO MINEIRO DE MICROBIOLOGIA DE ALIMENTOS, 3.; FÓRUM DE DEBATES SOBRE VIGILÂNCIA SANITÁRIA, 3., 2008, Viçosa, MG. Qualidade, segurança alimentar e sustentabilidade: quando? onde? como? por que?: anais. Viçosa, MG: sbCTA, 2008.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
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