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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
05/07/2013 |
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Data da última atualização: |
19/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BRITTO, D. S.; PIROVANI, C. P.; ANDRADE, B. S.; SANTOS, T. P. dos; PUNGARTNIK, C.; CASCARDO, J. C. M.; MICHELI, F.; GESTEIRA, A. da S. |
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Afiliação: |
DAHYANA SANTOS BRITTO, UESC; CARLOS PRIMINHO PIROVANI, UESC; BRUNO SILVA ANDRADE, UESB; TASSIARA PEREIRA DOS SANTOS, UESC; CRISTINA PUNGARTNIK, UESC; JÚLIO CEZAR M. CASCARDO, UESC; FABIENNE MICHELI, CIRAD; ABELMON DA SILVA GESTEIRA, CNPMF. |
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Título: |
Recombinant b-1,3-1,4-glucanase from Theobroma cacao impairs Moniliophthora perniciosa mycelial growth. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Molecular Biology Reports, 2013. |
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ISSN: |
0301-4851 |
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DOI: |
10.1007/s11033-013-2640-1 |
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Idioma: |
Inglês |
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Conteúdo: |
In this work, we identified a gene from Theobroma cacao L. genome and cDNA libraries, named TcGlu2, that encodes a b-1,3-1,4-glucanase. The TcGlu2 ORF was 720 bp in length and encoded a polypeptide of 239 amino acids with a molecular mass of 25.58 kDa. TcGlu2 contains a conserved domain characteristic of b- 1,3-1,4-glucanases and presented high protein identity with b-1,3-1,4-glucanases from other plant species. Molecular modeling of TcGlu2 showed an active site of 13 amino acids typical of glucanase with b-1,3 and 1,4 action mode. The recombinant cDNA TcGlu2 obtained by heterologous expression in Escherichia coli and whose sequence was confirmed by mass spectrometry, has a molecular mass of about 22 kDa (with His-Tag) and showed antifungal activity against the fungus Moniliophthora perniciosa, causal agent of the witches? broom disease in cacao. The integrity of the hyphae membranes of M. perniciosa, incubated with protein TcGlu2, was analyzed with propidium iodide. After 1 h of incubation, a strong fluorescence emitted by the hyphae indicating the hydrolysis of the membrane by TcGlu2, was observed. To our knowledge, this is the first study of a cacao b-1,3-1,4-glucanase expression in heterologous system and the first analysis showing the antifungal activity of a b-1,3-1,4-glucanase, in particular against M. perniciosa. |
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Thesagro: |
Cacau. |
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Thesaurus Nal: |
Antifungal proteins; Moniliophthora perniciosa. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02123naa a2200265 a 4500
001 1961523
005 2023-05-19
008 2013 bl uuuu u00u1 u #d
022 $a0301-4851
024 7 $a10.1007/s11033-013-2640-1$2DOI
100 1 $aBRITTO, D. S.
245 $aRecombinant b-1,3-1,4-glucanase from Theobroma cacao impairs Moniliophthora perniciosa mycelial growth.$h[electronic resource]
260 $c2013
520 $aIn this work, we identified a gene from Theobroma cacao L. genome and cDNA libraries, named TcGlu2, that encodes a b-1,3-1,4-glucanase. The TcGlu2 ORF was 720 bp in length and encoded a polypeptide of 239 amino acids with a molecular mass of 25.58 kDa. TcGlu2 contains a conserved domain characteristic of b- 1,3-1,4-glucanases and presented high protein identity with b-1,3-1,4-glucanases from other plant species. Molecular modeling of TcGlu2 showed an active site of 13 amino acids typical of glucanase with b-1,3 and 1,4 action mode. The recombinant cDNA TcGlu2 obtained by heterologous expression in Escherichia coli and whose sequence was confirmed by mass spectrometry, has a molecular mass of about 22 kDa (with His-Tag) and showed antifungal activity against the fungus Moniliophthora perniciosa, causal agent of the witches? broom disease in cacao. The integrity of the hyphae membranes of M. perniciosa, incubated with protein TcGlu2, was analyzed with propidium iodide. After 1 h of incubation, a strong fluorescence emitted by the hyphae indicating the hydrolysis of the membrane by TcGlu2, was observed. To our knowledge, this is the first study of a cacao b-1,3-1,4-glucanase expression in heterologous system and the first analysis showing the antifungal activity of a b-1,3-1,4-glucanase, in particular against M. perniciosa.
650 $aAntifungal proteins
650 $aMoniliophthora perniciosa
650 $aCacau
700 1 $aPIROVANI, C. P.
700 1 $aANDRADE, B. S.
700 1 $aSANTOS, T. P. dos
700 1 $aPUNGARTNIK, C.
700 1 $aCASCARDO, J. C. M.
700 1 $aMICHELI, F.
700 1 $aGESTEIRA, A. da S.
773 $tMolecular Biology Reports, 2013.
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| 1. |  | VILAS BOAS, A. L. G.; ANDRADE, A. C. M.; SANTOS, E. A.; BASTOS, L. P.; BARBOSA, C. de J.; BASTOS, M. J. S. M. Estabelecimento in vitro sisal (Agave Sisalana) procedente do território do sisal na Bahia. In: REUNIÃO ANUAL DE CIÊNCIA, TECNOLOGIA, INOVAÇÃO E CULTURA NO RECÔNCAVO DA BAHIA - RECITEC RECÔNCAVO. 1., 2011, Cruz das Almas. Anais... Cruz das Almas: Universidade Federal do Recôncavo da Bahia, 2011. 1 CD-ROM. 2 p. Em paralelo aconteceram também os seguintes eventos: V Seminário de Pesquisa do Recôncavo da Bahia; V Seminário Estudantil de Pesquisa da UFRB; V Seminário da Pós-Graduação da UFRB; II Seminário Regional de Pesquisa da EBDA; 5ª Jornada...| Tipo: Resumo em Anais de Congresso |
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