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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
27/06/2013 |
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Data da última atualização: |
19/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SILVA, A. dos S.; OLIVEIRA, E. J. de; HADDAD, F.; JESUS, O. N. de; OLIVEIRA, S. A. S. de; COSTA, M. A. P. de C. |
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Afiliação: |
ALINE DOS SANTOS SILVA, UFRB; EDER JORGE DE OLIVEIRA, CNPMF; FERNANDO HADDAD, CNPMF; ONILDO NUNES DE JESUS, CNPMF; SAULO ALVES SANTOS DE OLIVEIRA, CNPMF; MARIA ANGÉLICA PEREIRA DE CARVALHO COSTA, UFRB. |
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Título: |
Molecular fingerprinting of Fusarium oxysporum f. sp. passiflorae isolates using AFLP markers. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Scientia Agricola, v.70, n.2, p.108-115, March/April 2013. |
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ISSN: |
0103-9016 |
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Idioma: |
Português |
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Conteúdo: |
Fusarium oxysporum f. sp. passiflorae W.L. Gordon (FOP) is one of the most important fungal pathogens of passion fruits. Understanding molecular variation of isolates from different areas is of utmost importance. Molecular fingerprinting on 14 isolates of FOP were conducted using AFLP molecular markers (Amplified Fragment Length Polymorphism), and their genetic variability were estimated. Twenty-five AFLP primer combinations were selected for amplification of FOP isolates and one for Fusarium oxysporum f. sp. cubense W.C. Snyder & H.N. Hansen (FOC), resulting in 99% polymorphic fragments, with an average of 40 fragments per primer combination. Specific fingerprints could be generated for most of the isolates evaluated; we observed a high power of discrimination of the AFLP primer combinations, with the presence/absence of up to 26 specific fragments per isolate. Thus, specific fingerprinting was obtained for 10 of the 15 isolates analyzed. |
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Palavras-Chave: |
Passion fruit. |
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Thesagro: |
Fusarium; Maracujá; Marcador Molecular. |
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Thesaurus Nal: |
Genetic markers. |
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Categoria do assunto: |
-- |
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Marc: |
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022 $a0103-9016
100 1 $aSILVA, A. dos S.
245 $aMolecular fingerprinting of Fusarium oxysporum f. sp. passiflorae isolates using AFLP markers.$h[electronic resource]
260 $c2013
520 $aFusarium oxysporum f. sp. passiflorae W.L. Gordon (FOP) is one of the most important fungal pathogens of passion fruits. Understanding molecular variation of isolates from different areas is of utmost importance. Molecular fingerprinting on 14 isolates of FOP were conducted using AFLP molecular markers (Amplified Fragment Length Polymorphism), and their genetic variability were estimated. Twenty-five AFLP primer combinations were selected for amplification of FOP isolates and one for Fusarium oxysporum f. sp. cubense W.C. Snyder & H.N. Hansen (FOC), resulting in 99% polymorphic fragments, with an average of 40 fragments per primer combination. Specific fingerprints could be generated for most of the isolates evaluated; we observed a high power of discrimination of the AFLP primer combinations, with the presence/absence of up to 26 specific fragments per isolate. Thus, specific fingerprinting was obtained for 10 of the 15 isolates analyzed.
650 $aGenetic markers
650 $aFusarium
650 $aMaracujá
650 $aMarcador Molecular
653 $aPassion fruit
700 1 $aOLIVEIRA, E. J. de
700 1 $aHADDAD, F.
700 1 $aJESUS, O. N. de
700 1 $aOLIVEIRA, S. A. S. de
700 1 $aCOSTA, M. A. P. de C.
773 $tScientia Agricola$gv.70, n.2, p.108-115, March/April 2013.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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| 1. |  | FUKUDA, W.; SANTOS, V.; OLIVEIRA, L.; PEREIRA, M.; CEBALLOS, H.; NUTTI, M.; CARVALHO, J.; DITA, MIGUEL. Breeding cassava for enhancement of carotenoid, iron and zinc contents. In: SCIENTIFIC MEETING OF THE GLOBAL CASSAVA PARTNERSHIP, 1., 2008, Ghent. Cassava: meeting the challenges of the new millennium.Ghent:: IPBO, 2008. p. 106. S7-9.| Tipo: Resumo em Anais de Congresso |
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