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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
20/02/2013 |
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Data da última atualização: |
19/06/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ABREU, E. F. M.; TINOCO, M. L. P.; ANDRADE, E. C. de; ARAGAO, F. J. L. |
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Afiliação: |
EMANUEL FELIPE MEDEIROS ABREU, CNPMF; MARIA LAINE PENHA TINOCO, CENARGEN; EDUARDO CHUMBINHO DE ANDRADE, SRI; FRANCISCO JOSE LIMA ARAGAO, CENARGEN. |
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Título: |
Diversity among isolates of cowpea severe mosaic virus infecting cowpeas in northeastern Brazil. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 11, n. 3, p. 3146-3153, 2012. |
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ISSN: |
1676-5680 |
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Idioma: |
Inglês |
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Conteúdo: |
Eleven isolates of cowpea severe mosaic virus (CPSMV), a member of the genus Comovirus, were selected from 50 samples collected of nine cowpea fields in Northeastern Brazil (Piauí, Ceará, Rio Grande do Norte, Paraíba, Pernambuco, Alagoas, Sergipe, Bahia, and Distrito Federal) and partially sequenced. The RNA1 partial sequence, corresponding to the helicase, viral genome-linked protein, picornain 3C-like protease, and the RNA-directed RNA polymerase genes from CPSMV, had high identity among isolates, varying from 98 to 100%. No evidence was found for intermolecular or intramolecular recombination. Phylogenetic analysis confirmed that the Brazilian CPSMV isolates are substantially different from the CPSMV strain USA. Despite the low variability found among Brazilian CPSMV isolates, there were notable differences in the symptomatology of infected cowpea plants, ranging from mild to moderate. Previous reports have demonstrated an association between CPSMV symptom determinants and helicase. However, we found no correlation between the helicase Diversity among isolates of cowpea severe mosaic virus 3147 mutations and symptoms caused by CPSMV. Nevertheless, all isolates with mutation R to K in the protease provoked severe symptoms. This type of information can provide a foundation for the development of strategies to produce durable resistant cowpea lines. It is crucial for strategies based on DNA sequence-dependent technologies, such as inhibition with RNAi. |
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Palavras-Chave: |
Isolate of cowpea. |
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Thesaurus Nal: |
Cowpea severe mosaic virus. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02092naa a2200193 a 4500
001 1950162
005 2023-06-19
008 2012 bl uuuu u00u1 u #d
022 $a1676-5680
100 1 $aABREU, E. F. M.
245 $aDiversity among isolates of cowpea severe mosaic virus infecting cowpeas in northeastern Brazil.$h[electronic resource]
260 $c2012
520 $aEleven isolates of cowpea severe mosaic virus (CPSMV), a member of the genus Comovirus, were selected from 50 samples collected of nine cowpea fields in Northeastern Brazil (Piauí, Ceará, Rio Grande do Norte, Paraíba, Pernambuco, Alagoas, Sergipe, Bahia, and Distrito Federal) and partially sequenced. The RNA1 partial sequence, corresponding to the helicase, viral genome-linked protein, picornain 3C-like protease, and the RNA-directed RNA polymerase genes from CPSMV, had high identity among isolates, varying from 98 to 100%. No evidence was found for intermolecular or intramolecular recombination. Phylogenetic analysis confirmed that the Brazilian CPSMV isolates are substantially different from the CPSMV strain USA. Despite the low variability found among Brazilian CPSMV isolates, there were notable differences in the symptomatology of infected cowpea plants, ranging from mild to moderate. Previous reports have demonstrated an association between CPSMV symptom determinants and helicase. However, we found no correlation between the helicase Diversity among isolates of cowpea severe mosaic virus 3147 mutations and symptoms caused by CPSMV. Nevertheless, all isolates with mutation R to K in the protease provoked severe symptoms. This type of information can provide a foundation for the development of strategies to produce durable resistant cowpea lines. It is crucial for strategies based on DNA sequence-dependent technologies, such as inhibition with RNAi.
650 $aCowpea severe mosaic virus
653 $aIsolate of cowpea
700 1 $aTINOCO, M. L. P.
700 1 $aANDRADE, E. C. de
700 1 $aARAGAO, F. J. L.
773 $tGenetics and Molecular Research$gv. 11, n. 3, p. 3146-3153, 2012.
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