| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Instrumentação; Embrapa Mandioca e Fruticultura. |
|
Data corrente: |
24/03/2011 |
|
Data da última atualização: |
29/01/2019 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
PEREIRA, F. M. V.; MILORI, D. M. B. P.; PEREIRA-FILHO, E. R.; VENANCIO, A. L.; RUSSO, M. de S. T.; MARTINS, P. K.; FREITAS-ASTUA, J. |
|
Afiliação: |
DEBORA MARCONDES BASTOS P MILORI, CNPDIA. |
|
Título: |
Fluorescence images combined to statistic test for fingerprinting of citrus plants after bacterial infection. |
|
Ano de publicação: |
2011 |
|
Fonte/Imprenta: |
Analytical methods, [S. l.], v. 3, p. 552-556, 2011. |
|
DOI: |
10.1039/C0AY00538J |
|
Idioma: |
Inglês |
|
Conteúdo: |
The citrus greening (or huanglongbing) disease has caused serious problems in citrus crops around the world. An early diagnostic method to detect this malady is needed due to the rapid dissemination of Candidatus Liberibacter asiaticus (CLas) in the field. This analytical study investigated the fluorescence responses of leaves from healthy citrus plants and those inoculated with CLas by images from a stereomicroscope and also evaluated their potential for the early diagnosis of the infection caused by this bacterium. The plants were measured monthly, and the evolution of the bacteria on inoculated plants was monitored by real-time quantitative polymerase chain reaction (RT-qPCR) amplication of CLas sequences. A statistical method was used to analyse the data. The selection of variables from histograms of colours (colourgrams) of the images was optimized using a paired Student 's t-test. The intensity of counts for green colours from images of fluorescence had clearly minor variations for healthy plants than diseased ones. The darker green colours were the indicators of healthy plants and the light colours for the diseased. The method of fluorescence images is novel for fingerprinting healthy and diseased plants and provides an alternative to the current method represented by PCR and visual inspection. A new, non-subjective pattern of analysis and a non-destructive method has been introduced that can minimize the time and costs of analyses. |
|
Palavras-Chave: |
Citrus greening disease; Doença do greening citrus; Huanglongbing; Method of fluorescence images; Método de imagens de fluorescência; Plant disease; Statistical method. |
|
Thesagro: |
Bactéria; Greening; Método Estatístico. |
|
Thesaurus Nal: |
Candidatus Liberibacter. |
|
Categoria do assunto: |
--
X Pesquisa, Tecnologia e Engenharia |
|
Marc: |
LEADER 02525naa a2200337 a 4500
001 1900658
005 2019-01-29
008 2011 bl uuuu u00u1 u #d
024 7 $a10.1039/C0AY00538J$2DOI
100 1 $aPEREIRA, F. M. V.
245 $aFluorescence images combined to statistic test for fingerprinting of citrus plants after bacterial infection.$h[electronic resource]
260 $c2011
520 $aThe citrus greening (or huanglongbing) disease has caused serious problems in citrus crops around the world. An early diagnostic method to detect this malady is needed due to the rapid dissemination of Candidatus Liberibacter asiaticus (CLas) in the field. This analytical study investigated the fluorescence responses of leaves from healthy citrus plants and those inoculated with CLas by images from a stereomicroscope and also evaluated their potential for the early diagnosis of the infection caused by this bacterium. The plants were measured monthly, and the evolution of the bacteria on inoculated plants was monitored by real-time quantitative polymerase chain reaction (RT-qPCR) amplication of CLas sequences. A statistical method was used to analyse the data. The selection of variables from histograms of colours (colourgrams) of the images was optimized using a paired Student 's t-test. The intensity of counts for green colours from images of fluorescence had clearly minor variations for healthy plants than diseased ones. The darker green colours were the indicators of healthy plants and the light colours for the diseased. The method of fluorescence images is novel for fingerprinting healthy and diseased plants and provides an alternative to the current method represented by PCR and visual inspection. A new, non-subjective pattern of analysis and a non-destructive method has been introduced that can minimize the time and costs of analyses.
650 $aCandidatus Liberibacter
650 $aBactéria
650 $aGreening
650 $aMétodo Estatístico
653 $aCitrus greening disease
653 $aDoença do greening citrus
653 $aHuanglongbing
653 $aMethod of fluorescence images
653 $aMétodo de imagens de fluorescência
653 $aPlant disease
653 $aStatistical method
700 1 $aMILORI, D. M. B. P.
700 1 $aPEREIRA-FILHO, E. R.
700 1 $aVENANCIO, A. L.
700 1 $aRUSSO, M. de S. T.
700 1 $aMARTINS, P. K.
700 1 $aFREITAS-ASTUA, J.
773 $tAnalytical methods, [S. l.]$gv. 3, p. 552-556, 2011.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Instrumentação (CNPDIA) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 1 | |
| 1. |  | REIS, T. C.; FREITAS, I. S.; NUNES, T.; SANTANA, D. H. S. de; CRUZ, M. F. S.; MACHADO, C. de F.; LEDO, C. A. da S. Caracterização morfológica e agronômica de acessos de Spondias utilizando descritores quantitativos e qualitativos. In : JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA, 12., 2018. Ciência profissional : resumos. Cruz das Almas, BA: Embrapa Mandioca e Fruticultura, 2019. 1 p.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
|    |
| Registros recuperados : 1 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
