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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
28/04/2011 |
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Data da última atualização: |
29/04/2011 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
NEVES, D. M.; COELHO FILHO, M. A.; SOUZA, D. T.; MENDES, A. F. S.; COSTA, M. G. C.; SOARES FILHO, W. dos S.; GESTEIRA, A. da S. |
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Afiliação: |
Diana Matos Neves, UESC; MAURICIO ANTONIO COELHO FILHO, CNPMF; D. T. Souza, UFV; Amanda Ferreira da Silva Mendes, UESC; Marcio Gilberto Cardoso Costa, UESC; WALTER DOS SANTOS SOARES FILHO, CNPMF; ABELMON DA SILVA GESTEIRA, CNPMF. |
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Título: |
Functional analysis of 9-cis-epoxycarotenoid dioxygenase (NCEDs) genes in response to water deficit in citrus. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 3, 2011, Ilhéus. Resumos. [S. l.]: Sociedade Brasileira de Genética, 2011. 1 CD-ROM. |
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Idioma: |
Inglês |
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Notas: |
pdf 34672 |
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Conteúdo: |
Abscisic acid (ABA) regulates plant adaptive responses to various environmental stresses. The oxidative cleavage of cis-epoxycarotenoids catalyzed by 9-cis-epoxycarotenoid dioxygenase (NCED) is considered to be the rate-limiting step in abscisic acid (ABA) biosynthesis. Here, we analyzed the expression of five NCEDs genes by RT-qPCR in roots and leaves of Rangpur lime (Citrus limonia Osb.) and Sunki mandarin (C. sunki) grown under water deficit. Based on the normalized transpiration rate and fraction of transpirable soil water, we determined three assessment points: 1) start of the water deficit; 2) intermediate stage of water deficit; and 3) permanent wilting point. In water stressed Rangpur lime, NCED2 was strongly induced in leaves and the NCED2, NCED3 and NCED4 were strongly induced in roots. Interestingly, NCED4 showed an expression approximately 15 times higher at permanent wilting point. In Sunki mandarin leaves, no significant differential expression was observed for the 5 genes, whatever the water deficit condition (stressed vs unstressed). However, in Sunki mandarin roots, NCED2, NCED4 and NCED5 were strongly induced at the permanent wilting point (23, 18 and 10 times more in plants stressed, respectively). These results suggest the involvement of the NCED enzymes in controlling the ABA level in roots when subjected to water deficit, and indicate that the multigene family of NCEDs could be considered as good candidate for analysis ABA-mediated drought in Citrus. |
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Palavras-Chave: |
Drought deficit. |
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Thesaurus Nal: |
abscisic acid; Citrus. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02276nam a2200229 a 4500
001 1887059
005 2011-04-29
008 2011 bl uuuu u00u1 u #d
100 1 $aNEVES, D. M.
245 $aFunctional analysis of 9-cis-epoxycarotenoid dioxygenase (NCEDs) genes in response to water deficit in citrus.
260 $aIn: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 3, 2011, Ilhéus. Resumos. [S. l.]: Sociedade Brasileira de Genética, 2011. 1 CD-ROM.$c2011
500 $apdf 34672
520 $aAbscisic acid (ABA) regulates plant adaptive responses to various environmental stresses. The oxidative cleavage of cis-epoxycarotenoids catalyzed by 9-cis-epoxycarotenoid dioxygenase (NCED) is considered to be the rate-limiting step in abscisic acid (ABA) biosynthesis. Here, we analyzed the expression of five NCEDs genes by RT-qPCR in roots and leaves of Rangpur lime (Citrus limonia Osb.) and Sunki mandarin (C. sunki) grown under water deficit. Based on the normalized transpiration rate and fraction of transpirable soil water, we determined three assessment points: 1) start of the water deficit; 2) intermediate stage of water deficit; and 3) permanent wilting point. In water stressed Rangpur lime, NCED2 was strongly induced in leaves and the NCED2, NCED3 and NCED4 were strongly induced in roots. Interestingly, NCED4 showed an expression approximately 15 times higher at permanent wilting point. In Sunki mandarin leaves, no significant differential expression was observed for the 5 genes, whatever the water deficit condition (stressed vs unstressed). However, in Sunki mandarin roots, NCED2, NCED4 and NCED5 were strongly induced at the permanent wilting point (23, 18 and 10 times more in plants stressed, respectively). These results suggest the involvement of the NCED enzymes in controlling the ABA level in roots when subjected to water deficit, and indicate that the multigene family of NCEDs could be considered as good candidate for analysis ABA-mediated drought in Citrus.
650 $aabscisic acid
650 $aCitrus
653 $aDrought deficit
700 1 $aCOELHO FILHO, M. A.
700 1 $aSOUZA, D. T.
700 1 $aMENDES, A. F. S.
700 1 $aCOSTA, M. G. C.
700 1 $aSOARES FILHO, W. dos S.
700 1 $aGESTEIRA, A. da S.
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