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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
18/10/2010 |
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Data da última atualização: |
22/08/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
DITA, M. A.; WAALWIJK, C.; BUDDENHAGEN, I. M.; SOUZA JUNIOR, M. T.; KEMA, G. H. J. |
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Afiliação: |
MIGUEL ANGEL DITA RODRIGUEZ, CNPMF; C. Waalwijk, Plant Research International; I. W. Buddenhagen, Plum Lane; MANOEL TEIXEIRA SOUZA JUNIOR, CNPAE; Gert H. J. Kema, Plant Research International. |
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Título: |
A molecular diagnostic for tropical race 4 of the banana fusarium wilt pathogen. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Plant Pathology, Dordrecht, v. 59, p. 348?357, 2010. |
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DOI: |
10.1111/j.1365-3059.2009.02221.x |
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Idioma: |
Inglês |
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Notas: |
Disponível em: Acesso em: 26 out. 2010. |
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Conteúdo: |
This study analysed genomic variation of the translation elongation factor 1a (TEF-1a) and the intergenic spacer region (IGS) of the nuclear ribosomal operon of Fusarium oxysporum f. sp. cubense (Foc) isolates, from different banana production areas, representing strains within the known races, comprising 20 vegetative compatibility groups (VCG). Based on two single nucleotide polymorphisms present in the IGS region, a PCR-based diagnostic tool was developed to specifically detect isolates from VCG 01213, also called tropical race 4 (TR4), which is currently a major concern in global banana production. Validation involved TR4 isolates, as well as Foc isolates from 19 other VCGs, other fungal plant pathogens and DNA samples from infected tissues of the Cavendish banana cultivar Grand Naine (AAA). Subsequently, a multiplex PCR was developed for fungal or plant samples that also discriminated Musa acuminata and M. balbisiana genotypes. It was concluded that this diagnostic procedure is currently the best option for the rapid and reliable detection and monitoring of TR4 to support eradication and quarantine strategies. |
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Palavras-Chave: |
Musa spp. |
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Thesagro: |
Banana; Doença de Planta; Fungo; Fusarium Oxysporum; Mal do Panamá. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 01999naa a2200265 a 4500
001 1864489
005 2022-08-22
008 2010 bl uuuu u00u1 u #d
024 7 $a10.1111/j.1365-3059.2009.02221.x$2DOI
100 1 $aDITA, M. A.
245 $aA molecular diagnostic for tropical race 4 of the banana fusarium wilt pathogen.$h[electronic resource]
260 $c2010
500 $aDisponível em: <http://onlinelibrary.wiley.com/doi/10.1111/j.1365-3059.2009.02221.x/pdf> Acesso em: 26 out. 2010.
520 $aThis study analysed genomic variation of the translation elongation factor 1a (TEF-1a) and the intergenic spacer region (IGS) of the nuclear ribosomal operon of Fusarium oxysporum f. sp. cubense (Foc) isolates, from different banana production areas, representing strains within the known races, comprising 20 vegetative compatibility groups (VCG). Based on two single nucleotide polymorphisms present in the IGS region, a PCR-based diagnostic tool was developed to specifically detect isolates from VCG 01213, also called tropical race 4 (TR4), which is currently a major concern in global banana production. Validation involved TR4 isolates, as well as Foc isolates from 19 other VCGs, other fungal plant pathogens and DNA samples from infected tissues of the Cavendish banana cultivar Grand Naine (AAA). Subsequently, a multiplex PCR was developed for fungal or plant samples that also discriminated Musa acuminata and M. balbisiana genotypes. It was concluded that this diagnostic procedure is currently the best option for the rapid and reliable detection and monitoring of TR4 to support eradication and quarantine strategies.
650 $aBanana
650 $aDoença de Planta
650 $aFungo
650 $aFusarium Oxysporum
650 $aMal do Panamá
653 $aMusa spp
700 1 $aWAALWIJK, C.
700 1 $aBUDDENHAGEN, I. M.
700 1 $aSOUZA JUNIOR, M. T.
700 1 $aKEMA, G. H. J.
773 $tPlant Pathology, Dordrecht$gv. 59, p. 348?357, 2010.
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