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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
25/02/2010 |
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Data da última atualização: |
29/05/2023 |
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Tipo da produção científica: |
Artigo de Divulgação na Mídia |
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Autoria: |
FERREIRA, C. F.; VAN DER LEE, T. A. J.; QUIROS, O.; TE LINTEL, H.; ZAPATER, M.; CARLIER, J.; GOODWIN, S.; GUZMAN, M.; SOUZA JÚNIOR, M.; KEMA, G. H. J. |
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Afiliação: |
CLAUDIA FORTES FERREIRA, CNPMF; Theodoor Angelo Jozef Van Der Lee, PRIN; Olman Quirós, CORBANA; Bas Te Lintel Hekkert, PRIN; Marie Francoise Zapater, CIRAD; Jean Carlier, CIRAD; Stephen B. Goodwin; Mauricio Guzman, CORBANA; Manoel Teixeira Souza Júnior, PRIN; Gert H. J. Kema, PRIN. |
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Título: |
Development of VNTR markers to assess genetic diversity of Mycosphaerella fijiensis, the causal agent of black leaf streak disease in bananas (Musa spp.). |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
UNITED STATES DEPARTMENT OF AGRICULTURAL. Agricultural Research Service. Crop production and pest control research. |
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Idioma: |
Inglês |
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Notas: |
Disponível em: <http://www.ars.usda.gov/research/publications/publications.htm?seq_no_115=231337 >. Acesso em: 25 fev. 2010 |
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Conteúdo: |
Mycosphaerella fijiensis, the causal agent of black leaf streak disease of banana, commonly known as Black Sigatoka, is one of the most devastating plant pathogens. Black leaf streak was first discovered in Fiji during the 1960s and the pathogen still actively colonizes new areas where bananas are grown. Using the genome sequence of M. fijiensis isolate CIRAD86 (http://genome.jgi-psf.org/Mycfi1/Mycfi1.home.html), we identified Variable Number of Tandem Repeat (VNTR) markers using a bioinformatics pipeline. The VNTR screening resulted in 1528 candidate VNTR loci of which many were imperfect mono-, di-, or tri-nucleotide repeats. Sixteen VNTR markers were selected based on repeat type, repeat length and the number of units of the repeat. Primers for these 16 VNTR markers were tested on a set of M. fijiensis isolates and five VNTR markers that showed multiple alleles were tested on M. fijiensis field isolates and on progeny of the cross between isolates CIRAD86 and CIRAD 139a. Numbers of alleles identified varied from 2 to 5 per locus. The markers allowed high throughput and robust scoring on agarose gels and proved useful for genetic diversity and population genetic studies using basic molecular instrumentation. We envisage that this set will enable more detailed analyses of the large-scale migration pattern of this important pathogen as well as small-scale effects of fungicide treatments on the population diversity in banana plantations. |
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Palavras-Chave: |
Melhoramento genético. |
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Thesagro: |
Doença de Planta; Fungo. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02491naa a2200277 a 4500
001 1658910
005 2023-05-29
008 2008 bl uuuu u00u1 u #d
100 1 $aFERREIRA, C. F.
245 $aDevelopment of VNTR markers to assess genetic diversity of Mycosphaerella fijiensis, the causal agent of black leaf streak disease in bananas (Musa spp.).$h[electronic resource]
260 $c2008
500 $aDisponível em: <http://www.ars.usda.gov/research/publications/publications.htm?seq_no_115=231337 >. Acesso em: 25 fev. 2010
520 $aMycosphaerella fijiensis, the causal agent of black leaf streak disease of banana, commonly known as Black Sigatoka, is one of the most devastating plant pathogens. Black leaf streak was first discovered in Fiji during the 1960s and the pathogen still actively colonizes new areas where bananas are grown. Using the genome sequence of M. fijiensis isolate CIRAD86 (http://genome.jgi-psf.org/Mycfi1/Mycfi1.home.html), we identified Variable Number of Tandem Repeat (VNTR) markers using a bioinformatics pipeline. The VNTR screening resulted in 1528 candidate VNTR loci of which many were imperfect mono-, di-, or tri-nucleotide repeats. Sixteen VNTR markers were selected based on repeat type, repeat length and the number of units of the repeat. Primers for these 16 VNTR markers were tested on a set of M. fijiensis isolates and five VNTR markers that showed multiple alleles were tested on M. fijiensis field isolates and on progeny of the cross between isolates CIRAD86 and CIRAD 139a. Numbers of alleles identified varied from 2 to 5 per locus. The markers allowed high throughput and robust scoring on agarose gels and proved useful for genetic diversity and population genetic studies using basic molecular instrumentation. We envisage that this set will enable more detailed analyses of the large-scale migration pattern of this important pathogen as well as small-scale effects of fungicide treatments on the population diversity in banana plantations.
650 $aDoença de Planta
650 $aFungo
653 $aMelhoramento genético
700 1 $aVAN DER LEE, T. A. J.
700 1 $aQUIROS, O.
700 1 $aTE LINTEL, H.
700 1 $aZAPATER, M.
700 1 $aCARLIER, J.
700 1 $aGOODWIN, S.
700 1 $aGUZMAN, M.
700 1 $aSOUZA JÚNIOR, M.
700 1 $aKEMA, G. H. J.
773 $tUNITED STATES DEPARTMENT OF AGRICULTURAL. Agricultural Research Service. Crop production and pest control research.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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| Registros recuperados : 4 | |
| 1. |  | SILVA, D. J.; SIGNOR, D.; DEON, M. D.; SILVA, J. A. da; BARROS, J. Q.; LIMA, P. C. P. Avaliação do efeito de aceleradores de compostagem no cultivo de plantas de sorgo. In: REUNIÃO NORDESTINA DE CIÊNCIA DO SOLO, 3., 2017, Aracaju. Ciência do solo: integração e uso do conhecimento para uma agricultura sustentável no Nordeste - anais. Aracaju: Embrapa Tabuleiros Costeiros: Unit, 2017.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| 2. | | CALIXTO JÚNOR, J. T.; DRUMOND, M. A.; SILVA, J. A. da; SA, I. B.; OLIVEIRA, V. R. de. Estrutura fitossociológica de um fragmento de caatinga sensu stricto no sertão de Pernambuco. In: CONGRESSO NORDESTINO DE ENGENHARIA FLORESTAL, 2.; SIMPÓSIO DA PÓS-GRADUAÇÃO EM CIÊNCIAS FLORESTAIS DA UFCG, 1.; SEMANA DA ENGENHARIA FLORESTAL, 11., 2009, Campina Grande. Evolução e perspectivas da engenharia florestal no Nordeste. Campina Grande: UFCG, 2009. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Semiárido. |
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| 3. | | SILVA, J. A. da; SOUSA, F. A. de; LIMA, A. M. S.; FIGUEIREDO, R. W. de; LIMA, M. A. C. de; RUFINI, M. do S. M. Macromolecular antioxidants usually ignored, are the major part of dietary polyphenols: a study in the grapes industrial residues. International Journal of Development Research, v. 10, n. 8, p. 39527-39533, 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Semiárido. |
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| 4. | | LIMA, A. M. S.; SOUSA, F. A. de; SILVA, J, A. da; FIGUEIREDO, R. W. de; LIMA, M. A. C. de; RUFINO, M. do S. M. Prospecting for macromolecular antioxidants or non-extractable polyphenols in bagasse from wineries in the São Francisco River Valley. International Journal Of Development Research, v. 10, p. 37578-37582, 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Semiárido. |
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| Registros recuperados : 4 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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